Project name: nih4546 m2

Chain sequence(s)	H: VRLSQSGGQMKKPGESMRLSCRASGYEFLNCPINWIRLAPGRRPEWMGWLKPRWGAVNYARKFQGRVTMTRDVYSDTAFLELRSLTSDDDTAVYFCTRGKYCTARDYYNWDFEHWGRGAPVTVSSASTKGPSVFPLAPSSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPK; L: VLTQSPATLSLSPGETAIISCRTSQYGSLAWYQQRPGQAPRLVIYSGSTRAAGIPDRFSGSRWGADYNLSISNLESGDFGVYYCQQYEFFGQGTKVQVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE
Distance of aggregation	10 Å
Dynamic mode	No

• Minimal score value: -3.5146
• Maximal score value: 1.6598
• Average score: -0.7054
• Total score value: -301.9051

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
2	V	H	0.1377
3	R	H	-1.6825
4	L	H	0.0000
5	S	H	-1.4634
6	Q	H	-1.4181
7	S	H	-1.1971
8	G	H	-1.1099
9	G	H	-0.9250
10	Q	H	-0.6908
11	M	H	-0.0126
12	K	H	-0.9930
13	K	H	-1.5505
14	P	H	-1.6173
15	G	H	-1.5609
16	E	H	-1.8317
17	S	H	-1.7778
18	M	H	0.0000
19	R	H	-2.2109
20	L	H	0.0000
21	S	H	-0.9586
22	C	H	0.0000
23	R	H	-2.0735
24	A	H	0.0000
25	S	H	-1.1892
26	G	H	-0.6713
27	Y	H	-0.7719
28	E	H	-1.5798
29	F	H	0.0000
30	L	H	-0.4958
31	N	H	-1.0621
32	C	H	0.0000
33	P	H	0.0000
34	I	H	0.0000
35	N	H	0.0000
36	W	H	0.0000
37	I	H	0.0000
38	R	H	0.0000
39	L	H	0.0000
40	A	H	-1.3390
41	P	H	-1.1968
42	G	H	-2.0591
43	R	H	-3.1245
44	R	H	-2.7729
45	P	H	0.0000
46	E	H	-0.9452
47	W	H	0.0000
48	M	H	0.0000
49	G	H	0.0000
50	W	H	-0.1181
51	L	H	0.0000
52	K	H	-0.6643
52A	P	H	0.0000
53	R	H	-1.2386
54	W	H	0.3239
55	G	H	-0.0877
56	A	H	-0.1417
57	V	H	0.0651
58	N	H	-1.1882
59	Y	H	-1.3581
60	A	H	-1.9065
61	R	H	-3.1792
62	K	H	-3.0342
63	F	H	0.0000
64	Q	H	-2.6817
65	G	H	-1.7967
66	R	H	-1.5964
67	V	H	0.0000
68	T	H	-0.7205
69	M	H	0.0000
70	T	H	-0.1541
71	R	H	-0.7813
72	D	H	-0.6895
73	V	H	0.4770
74	Y	H	1.1463
75	S	H	-0.0366
76	D	H	-0.8681
77	T	H	-0.9029
78	A	H	0.0000
79	F	H	-0.7164
80	L	H	0.0000
81	E	H	-1.4917
82	L	H	0.0000
82A	R	H	-1.6731
82B	S	H	-1.2863
82C	L	H	0.0000
83	T	H	-1.4459
84	S	H	-1.4152
85	D	H	-1.9437
86	D	H	0.0000
87	T	H	-0.6685
88	A	H	0.0000
89	V	H	-0.1096
90	Y	H	0.0000
91	F	H	0.0000
92	C	H	0.0000
93	T	H	0.0000
94	R	H	0.0000
95	G	H	0.0000
96	K	H	-0.3299
97	Y	H	0.4363
98	C	H	-0.1612
99	T	H	-0.8284
99A	A	H	-1.3044
99B	R	H	-2.2404
99C	D	H	-1.3176
99D	Y	H	0.2706
100	Y	H	1.4450
101	N	H	0.0000
102	W	H	0.7621
103	D	H	0.0114
104	F	H	0.0000
105	E	H	-0.7001
106	H	H	-0.8702
107	W	H	-0.8024
108	G	H	0.0000
109	R	H	-2.3863
110	G	H	0.0000
111	A	H	0.0000
112	P	H	-0.5696
113	V	H	0.0000
114	T	H	-0.5857
115	V	H	0.0000
116	S	H	-0.8051
117	S	H	-0.7812
118	A	H	-0.5790
119	S	H	-0.5981
120	T	H	-0.6647
121	K	H	-1.2632
122	G	H	-1.4334
123	P	H	0.0000
124	S	H	-0.4203
125	V	H	0.0000
126	F	H	0.0000
127	P	H	-1.2692
128	L	H	0.0000
129	A	H	-1.2644
130	P	H	0.0000
131	S	H	-0.8129
132	S	H	-0.3874
137	G	H	-0.7825
138	G	H	-0.8908
139	T	H	-0.6731
140	A	H	-0.3562
141	A	H	0.0000
142	L	H	0.0000
143	G	H	0.0000
144	C	H	0.0000
145	L	H	0.0000
146	V	H	0.0000
147	K	H	0.0000
148	D	H	-0.5733
149	Y	H	0.0000
150	F	H	0.0000
151	P	H	-0.8294
152	E	H	-0.9711
153	P	H	-1.0492
154	V	H	-0.8559
155	T	H	-0.7050
156	V	H	-0.2482
157	S	H	-0.4343
158	W	H	0.0000
159	N	H	-0.7773
160	S	H	-0.6357
161	G	H	-0.4052
162	A	H	-0.1906
163	L	H	-0.0154
164	T	H	-0.1636
165	S	H	-0.1937
166	G	H	-0.2259
167	V	H	0.1538
168	H	H	-0.1149
169	T	H	0.0172
170	F	H	0.0000
171	P	H	-0.2962
172	A	H	0.1813
173	V	H	0.6358
174	L	H	1.2978
175	Q	H	0.2607
176	S	H	-0.0182
177	S	H	-0.1845
178	G	H	0.0745
179	L	H	0.0517
180	Y	H	0.3626
181	S	H	0.0000
182	L	H	0.0000
183	S	H	0.0000
184	S	H	0.0000
185	V	H	0.0000
186	V	H	0.0000
187	T	H	-0.1427
188	V	H	0.0000
189	P	H	-0.6700
190	S	H	-0.6816
191	S	H	-0.6348
192	S	H	-0.6460
193	L	H	-0.8533
194	G	H	-0.9923
195	T	H	-0.7563
196	Q	H	-1.2870
197	T	H	-1.1778
198	Y	H	0.0000
199	I	H	-1.5123
200	C	H	0.0000
201	N	H	-1.6103
202	V	H	0.0000
203	N	H	-2.1794
204	H	H	0.0000
205	K	H	-2.8706
206	P	H	-1.7487
207	S	H	-1.8212
208	N	H	-2.6749
209	T	H	-2.1321
210	K	H	-2.8216
211	V	H	-1.7945
212	D	H	-2.6857
213	K	H	-2.2900
214	R	H	-2.7493
215	V	H	0.0000
216	E	H	-2.9082
217	P	H	-1.9297
218	K	H	-2.6295
3	V	L	1.2916
4	L	L	0.0000
5	T	L	-0.6269
6	Q	L	0.0000
7	S	L	-0.6338
8	P	L	-0.2533
9	A	L	-0.4592
10	T	L	-0.4766
11	L	L	-0.0110
12	S	L	-0.2123
13	L	L	-0.4893
14	S	L	0.0000
15	P	L	-1.6766
16	G	L	-1.9269
17	E	L	-1.8257
18	T	L	-0.7671
19	A	L	0.0000
20	I	L	1.1152
21	I	L	0.0000
22	S	L	-0.7389
23	C	L	0.0000
24	R	L	-2.1106
25	T	L	0.0000
26	S	L	-0.4720
27	Q	L	-0.5827
28	Y	L	0.6415
29	G	L	0.2935
30	S	L	0.1568
31	L	L	0.0000
32	A	L	0.0000
33	W	L	0.0000
34	Y	L	0.0000
35	Q	L	-0.9659
36	Q	L	-1.4256
37	R	L	-1.9072
38	P	L	-1.3014
39	G	L	-1.5619
40	Q	L	-2.1401
41	A	L	-1.5909
42	P	L	0.0000
43	R	L	-1.3660
44	L	L	0.0000
45	V	L	0.0000
46	I	L	0.0000
47	Y	L	-0.4478
48	S	L	-0.4773
49	G	L	0.0000
50	S	L	-0.8680
51	T	L	-0.6225
52	R	L	-1.4671
53	A	L	-0.8224
54	A	L	-0.3687
55	G	L	-0.6558
56	I	L	-0.7874
57	P	L	-1.2402
58	D	L	-2.1908
59	R	L	-1.6431
60	F	L	0.0000
61	S	L	-0.8204
62	G	L	-0.8153
63	S	L	-1.2125
64	R	L	-1.9424
65	W	L	-0.3847
66	G	L	-0.6893
67	A	L	-1.0777
68	D	L	-1.5829
69	Y	L	0.0000
70	N	L	-0.9492
71	L	L	0.0000
72	S	L	0.0684
73	I	L	0.0000
74	S	L	-1.5204
75	N	L	-2.4344
76	L	L	0.0000
77	E	L	-2.4834
78	S	L	-1.2188
79	G	L	-1.2031
80	D	L	0.0000
81	F	L	0.0000
82	G	L	0.0000
83	V	L	-0.7246
84	Y	L	0.0000
85	Y	L	0.0000
86	C	L	0.0000
87	Q	L	0.0000
88	Q	L	0.0000
89	Y	L	1.6598
90	E	L	0.7200
91	F	L	1.2364
92	F	L	0.0000
93	G	L	0.0000
94	Q	L	-2.1737
95	G	L	0.0000
96	T	L	0.0000
97	K	L	-1.3953
98	V	L	0.0000
99	Q	L	-0.6122
100	V	L	-0.5816
101	D	L	-0.7989
102	I	L	-0.6197
103	K	L	-1.5002
104	R	L	-1.1666
105	T	L	-0.1225
106	V	L	0.6105
107	A	L	0.1501
108	A	L	-0.0581
109	P	L	0.0000
110	S	L	-0.1705
111	V	L	0.0000
112	F	L	0.1254
113	I	L	0.1433
114	F	L	0.0000
115	P	L	-0.6324
116	P	L	0.0000
117	S	L	-1.6379
118	D	L	-2.9141
119	E	L	-2.4876
120	Q	L	0.0000
121	L	L	-2.1181
122	K	L	-2.7319
123	S	L	-1.7071
124	G	L	-1.3304
125	T	L	-1.0423
126	A	L	0.0000
127	S	L	0.0000
128	V	L	0.0000
129	V	L	0.0000
130	C	L	0.0000
131	L	L	0.0000
132	L	L	0.0000
133	N	L	0.0000
134	N	L	-0.7992
135	F	L	0.0000
136	Y	L	0.0000
137	P	L	-1.4280
138	R	L	-2.4379
139	E	L	-2.8524
140	A	L	-2.0188
141	K	L	-1.9641
142	V	L	-0.8753
143	Q	L	-0.4866
144	W	L	0.0000
145	K	L	-0.5117
146	V	L	0.0000
147	D	L	-1.9632
148	N	L	-1.5190
149	A	L	-0.2578
150	L	L	0.7153
151	Q	L	-0.2265
152	S	L	-0.4948
153	G	L	-1.0605
154	N	L	-1.4061
155	S	L	-1.4252
156	Q	L	-1.5970
157	E	L	-1.5507
158	S	L	-0.8466
159	V	L	-0.6545
160	T	L	-1.1321
161	E	L	-2.1878
162	Q	L	-1.7117
163	D	L	-1.8788
164	S	L	-1.6384
165	K	L	-2.2578
166	D	L	-1.6719
167	S	L	-1.6655
168	T	L	0.0000
169	Y	L	0.0000
170	S	L	0.0000
171	L	L	0.0000
172	S	L	0.0000
173	S	L	0.0000
174	T	L	-0.7296
175	L	L	0.0000
176	T	L	-0.6109
177	L	L	-0.7484
178	S	L	-1.2298
179	K	L	-2.1037
180	A	L	-1.8389
181	D	L	-2.3189
182	Y	L	0.0000
183	E	L	-3.3082
184	K	L	-3.5146
185	H	L	-2.9318
186	K	L	-3.2661
187	V	L	-1.4574
188	Y	L	0.0000
189	A	L	-0.8053
190	C	L	0.0000
191	E	L	-0.8293
192	V	L	0.0000
193	T	L	-1.0634
194	H	L	-1.3820
195	Q	L	-1.6617
196	G	L	-0.4343
197	L	L	-0.2436
198	S	L	-0.4782
199	S	L	-0.4236
200	P	L	-0.5928
201	V	L	-0.0204
202	T	L	-0.4441
203	K	L	-0.7155
204	S	L	-0.4705
205	F	L	-0.8757
206	N	L	-1.9479
207	R	L	-2.6026
208	G	L	-2.3673
209	E	L	-2.6930