Project name: testcase

Chain sequence(s)	A: SSGLVPRGSHMGYSATAPVNLTRPATVPSMDGWTDGTGAWTLGEGTRVVSSDALAARAQSLASELTKFTDVDIKAATGSATGKDISLTLDASKKAELGDEGFKLNIGSKGLEVIGATDIGVFYGTRSVSQMLRQGQLTLPAGTVATKPKYKERGATLCACQINISTDWIDRFLSDMADLRLNYVLLEMKLKPEEDNTKKAATWSYYTRDDVKKFVKKANNYGIDVIPEINSPGHMNVWLENYPEYQLADNSGRKDPNKLDISNPEAVKFYKTLIDEYDGVFTTKYWHMGADEYMIGTSFDNYSKLKTFAEKQYGAGATPNDAFTGFINDIDKYVKAKGKQLRIWNDGIVNTKNVSLNKDIVIEYWYGAGRKPQELVQDGYTLMNATQALYWSRSAQVYKVNAARLYNNNWNVGTFDGGRQIDKNYDKLTGAKVSIWPDSSYFQTENEVEKEIFDGMRFISQMTWSDSRPWATWNDMKADIDKIGYPLDIREYDYTPVDAGIYDIPQLKSISKGPWELITTPDGYYQMKDTVSGKCLALFTGSKHLDVVTQVGARPELRNCADVSVGQDQRNTANERNTQKWQIRADKDGKYTISPALTQQRLAIATGNEQNIDLETHRPAAGTVAQFPADLVS
Distance of aggregation	10 Å
Dynamic mode	No

• Minimal score value: -4.4776
• Maximal score value: 1.798
• Average score: -0.7898
• Total score value: -499.9196

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
30	S	A	-0.2179
31	S	A	0.2020
32	G	A	0.5683
33	L	A	1.7980
34	V	A	1.7106
35	P	A	-0.1910
36	R	A	-1.6851
37	G	A	-1.4077
38	S	A	-1.2518
39	H	A	-1.5442
40	M	A	-0.9525
41	G	A	0.0000
42	Y	A	-0.3609
43	S	A	-0.1504
44	A	A	-0.0695
45	T	A	0.1203
46	A	A	0.1808
47	P	A	0.2677
48	V	A	0.8695
49	N	A	-0.5642
50	L	A	-0.2768
51	T	A	-0.7037
52	R	A	-1.5281
53	P	A	0.0000
54	A	A	-0.6730
55	T	A	0.0000
56	V	A	0.0000
57	P	A	0.0000
58	S	A	0.0000
59	M	A	-0.6518
60	D	A	-1.3451
61	G	A	-1.0486
62	W	A	-1.0460
63	T	A	-1.2681
64	D	A	-2.0163
65	G	A	-1.4464
66	T	A	-0.5242
67	G	A	-0.1882
68	A	A	0.0000
69	W	A	0.0000
70	T	A	0.0000
71	L	A	0.0000
72	G	A	0.0000
73	E	A	-2.7520
74	G	A	-1.9798
75	T	A	0.0000
76	R	A	-1.7925
77	V	A	0.0000
78	V	A	0.0000
79	S	A	0.0000
80	S	A	-1.0506
81	D	A	-1.9293
82	A	A	-0.9342
83	L	A	0.0000
84	A	A	-1.0901
85	A	A	-0.8079
86	R	A	-1.0671
87	A	A	0.0000
88	Q	A	-1.7945
89	S	A	-1.1197
90	L	A	0.0000
91	A	A	0.0000
92	S	A	-1.5868
93	E	A	0.0000
94	L	A	0.0000
95	T	A	-2.2015
96	K	A	-2.3227
97	F	A	-1.0327
98	T	A	-1.4891
99	D	A	-2.7373
100	V	A	-2.5308
101	D	A	-3.3453
102	I	A	0.0000
103	K	A	-2.5924
104	A	A	-1.4368
105	A	A	-1.0883
106	T	A	-0.8333
107	G	A	-0.8621
108	S	A	-0.4839
109	A	A	-0.8039
110	T	A	-0.8329
111	G	A	-1.1941
112	K	A	-1.3591
113	D	A	0.0000
114	I	A	0.0000
115	S	A	-0.6982
116	L	A	0.0000
117	T	A	-0.0200
118	L	A	0.1930
119	D	A	-0.7828
120	A	A	-1.1869
121	S	A	-1.3955
122	K	A	-2.5487
123	K	A	-3.2610
124	A	A	-2.1944
125	E	A	-3.0420
126	L	A	0.0000
127	G	A	-2.6792
128	D	A	-3.0759
129	E	A	-1.9368
130	G	A	0.0000
131	F	A	0.0000
132	K	A	-0.9032
133	L	A	0.0000
134	N	A	-1.0220
135	I	A	0.0000
136	G	A	-0.9723
137	S	A	-1.1371
138	K	A	-2.0889
139	G	A	0.0000
140	L	A	0.0000
141	E	A	-1.0847
142	V	A	0.0000
143	I	A	-0.2218
144	G	A	0.0000
145	A	A	0.0000
146	T	A	-1.1780
147	D	A	-1.0985
148	I	A	-0.7436
149	G	A	0.0000
150	V	A	0.0000
151	F	A	0.0000
152	Y	A	0.0000
153	G	A	0.0000
154	T	A	0.0000
155	R	A	0.0000
156	S	A	0.0000
157	V	A	0.0000
158	S	A	0.0000
159	Q	A	0.0000
160	M	A	0.0000
161	L	A	0.0000
162	R	A	0.0000
163	Q	A	-1.0410
164	G	A	-0.7229
165	Q	A	-0.4735
166	L	A	0.0862
167	T	A	0.0000
168	L	A	0.0000
169	P	A	-0.6917
170	A	A	0.0000
171	G	A	0.0000
172	T	A	-0.8915
173	V	A	0.0000
174	A	A	-0.9146
175	T	A	-1.3045
176	K	A	-2.3772
177	P	A	-1.7736
178	K	A	-1.8878
179	Y	A	0.0000
180	K	A	-2.2992
181	E	A	0.0000
182	R	A	0.0000
183	G	A	0.0000
184	A	A	0.0000
185	T	A	0.0000
186	L	A	0.0000
187	C	A	0.0000
188	A	A	0.0000
189	C	A	-0.1070
190	Q	A	-0.5902
191	I	A	0.0000
192	N	A	0.0000
193	I	A	0.0000
194	S	A	0.0000
195	T	A	-0.7108
196	D	A	-0.6070
197	W	A	0.0000
198	I	A	0.0000
199	D	A	-1.2166
200	R	A	0.0000
201	F	A	0.0000
202	L	A	0.0000
203	S	A	0.0000
204	D	A	0.0000
205	M	A	0.0000
206	A	A	0.0000
207	D	A	0.0000
208	L	A	0.0000
209	R	A	0.0000
210	L	A	0.0000
211	N	A	-0.6337
212	Y	A	0.0000
213	V	A	0.0000
214	L	A	0.0000
215	L	A	0.0000
216	E	A	-0.1846
217	M	A	0.0000
218	K	A	0.0000
219	L	A	0.0000
220	K	A	-1.4496
221	P	A	0.0000
222	E	A	-4.0135
223	E	A	-4.4776
224	D	A	-4.1906
225	N	A	-3.4441
226	T	A	0.0000
227	K	A	-3.6895
228	K	A	-2.5179
229	A	A	0.0000
230	A	A	0.0000
231	T	A	0.0000
232	W	A	0.0000
233	S	A	0.0000
234	Y	A	0.0000
235	Y	A	0.0000
236	T	A	-1.8447
237	R	A	-2.4018
238	D	A	-3.1731
239	D	A	-2.6759
240	V	A	0.0000
241	K	A	-3.1848
242	K	A	-3.6027
243	F	A	0.0000
244	V	A	0.0000
245	K	A	-3.2311
246	K	A	-2.6862
247	A	A	0.0000
248	N	A	-2.1112
249	N	A	-2.2305
250	Y	A	-1.1826
251	G	A	-0.9233
252	I	A	0.0000
253	D	A	-0.9381
254	V	A	0.0000
255	I	A	0.0000
256	P	A	0.0000
257	E	A	0.0000
258	I	A	0.0000
259	N	A	0.0000
260	S	A	0.0000
261	P	A	0.0000
262	G	A	0.0000
263	H	A	-0.6131
264	M	A	0.0000
265	N	A	-0.8479
266	V	A	-0.3383
267	W	A	0.0000
268	L	A	0.0000
269	E	A	-1.4334
270	N	A	-1.3648
271	Y	A	0.0000
272	P	A	-1.6957
273	E	A	-2.4941
274	Y	A	0.0000
275	Q	A	0.0000
276	L	A	0.0000
277	A	A	-2.3416
278	D	A	-2.5950
279	N	A	-2.7715
280	S	A	-1.9496
281	G	A	-2.2567
282	R	A	-3.0813
283	K	A	-2.9039
284	D	A	-1.8993
285	P	A	-1.6543
286	N	A	-1.0156
287	K	A	0.0000
288	L	A	0.0000
289	D	A	-1.1185
290	I	A	0.0000
291	S	A	-0.9145
292	N	A	-1.4659
293	P	A	-1.7259
294	E	A	-2.8938
295	A	A	0.0000
296	V	A	-1.5466
297	K	A	-2.4682
298	F	A	0.0000
299	Y	A	0.0000
300	K	A	-1.2616
301	T	A	-1.3973
302	L	A	0.0000
303	I	A	0.0000
304	D	A	-1.6922
305	E	A	0.0000
306	Y	A	0.0000
307	D	A	-1.6522
308	G	A	-1.2456
309	V	A	0.0000
310	F	A	0.0000
311	T	A	-1.1679
312	T	A	0.0000
313	K	A	-2.4275
314	Y	A	0.0000
315	W	A	0.0000
316	H	A	0.0000
317	M	A	0.0000
318	G	A	0.0000
319	A	A	0.0000
320	D	A	-0.2566
321	E	A	-0.1694
322	Y	A	0.0000
323	M	A	0.4195
324	I	A	1.5208
325	G	A	0.4302
326	T	A	-0.0855
327	S	A	-0.5220
328	F	A	-1.1352
329	D	A	-2.6689
330	N	A	-2.3337
331	Y	A	0.0000
332	S	A	-1.9785
333	K	A	-1.7439
334	L	A	0.0000
335	K	A	-2.9174
336	T	A	-2.2096
337	F	A	-1.9845
338	A	A	0.0000
339	E	A	-3.4281
340	K	A	-3.2677
341	Q	A	-2.6373
342	Y	A	-1.6865
343	G	A	-1.7434
344	A	A	-1.5812
345	G	A	-1.7420
346	A	A	0.0000
347	T	A	-1.6642
348	P	A	0.0000
349	N	A	-0.9704
350	D	A	0.0000
351	A	A	0.0000
352	F	A	0.0000
353	T	A	0.0000
354	G	A	0.0000
355	F	A	0.0000
356	I	A	0.0000
357	N	A	-1.2945
358	D	A	-1.3025
359	I	A	0.0000
360	D	A	0.0000
361	K	A	-2.5097
362	Y	A	-1.4415
363	V	A	0.0000
364	K	A	-3.1134
365	A	A	-1.5684
366	K	A	-1.9701
367	G	A	-1.8572
368	K	A	-2.3328
369	Q	A	-2.1520
370	L	A	0.0000
371	R	A	0.0000
372	I	A	0.0000
373	W	A	0.0000
374	N	A	0.0000
375	D	A	-0.3847
376	G	A	0.0000
377	I	A	-0.1909
378	V	A	-0.5371
379	N	A	-1.5651
380	T	A	-1.5781
381	K	A	-2.4054
382	N	A	-1.9932
383	V	A	-0.9853
384	S	A	-1.0620
385	L	A	-0.9095
386	N	A	-2.1565
387	K	A	-2.8714
388	D	A	-3.0364
389	I	A	0.0000
390	V	A	0.0000
391	I	A	0.0000
392	E	A	0.0000
393	Y	A	0.0000
394	W	A	0.0000
395	Y	A	0.2900
396	G	A	0.0000
397	A	A	-0.3256
398	G	A	-0.8632
399	R	A	-1.5076
400	K	A	-1.4968
401	P	A	0.0000
402	Q	A	-2.5637
403	E	A	-2.0988
404	L	A	0.0000
405	V	A	-1.7122
406	Q	A	-1.8767
407	D	A	-1.6475
408	G	A	-1.5875
409	Y	A	0.0000
410	T	A	-1.2737
411	L	A	0.0000
412	M	A	0.0000
413	N	A	0.0000
414	A	A	0.0000
415	T	A	0.0000
416	Q	A	0.0911
417	A	A	-0.4028
418	L	A	0.0000
419	Y	A	0.0000
420	W	A	0.0000
421	S	A	0.0000
422	R	A	0.0000
423	S	A	-0.3047
424	A	A	-0.1314
425	Q	A	-0.3566
426	V	A	1.2026
427	Y	A	0.3605
428	K	A	-0.4953
429	V	A	-0.8305
430	N	A	-1.8328
431	A	A	0.0000
432	A	A	-2.1538
433	R	A	-3.1756
434	L	A	0.0000
435	Y	A	0.0000
436	N	A	-3.1262
437	N	A	-3.0663
438	N	A	-2.6786
439	W	A	0.0000
440	N	A	-0.9228
441	V	A	0.0000
442	G	A	0.0000
443	T	A	-1.3565
444	F	A	0.0000
445	D	A	0.0000
446	G	A	-0.9381
447	G	A	-1.2290
448	R	A	-1.6625
449	Q	A	-2.2157
450	I	A	0.0000
451	D	A	-2.9721
452	K	A	-2.5480
453	N	A	-2.7877
454	Y	A	-2.3258
455	D	A	-2.8176
456	K	A	-2.0190
457	L	A	0.0000
458	T	A	0.0000
459	G	A	0.0000
460	A	A	0.0000
461	K	A	0.0000
462	V	A	0.0000
463	S	A	0.0000
464	I	A	0.0000
465	W	A	-0.0481
466	P	A	0.0000
467	D	A	-0.9375
468	S	A	-0.6498
469	S	A	0.0000
470	Y	A	-0.3523
471	F	A	-0.7714
472	Q	A	0.0000
473	T	A	0.0000
474	E	A	0.0000
475	N	A	0.0000
476	E	A	0.0000
477	V	A	0.0000
478	E	A	0.0000
479	K	A	-1.9109
480	E	A	-1.3502
481	I	A	0.0000
482	F	A	-0.8166
483	D	A	-1.1327
484	G	A	0.0000
485	M	A	0.0000
486	R	A	0.0000
487	F	A	0.0000
488	I	A	0.0000
489	S	A	0.0000
490	Q	A	0.0000
491	M	A	0.0000
492	T	A	0.0000
493	W	A	0.0000
494	S	A	-0.9342
495	D	A	-0.8516
496	S	A	-0.8313
497	R	A	-1.1857
498	P	A	-0.7653
499	W	A	-0.7595
500	A	A	-0.6611
501	T	A	-1.1664
502	W	A	0.0000
503	N	A	-2.0315
504	D	A	-1.7387
505	M	A	0.0000
506	K	A	-2.1046
507	A	A	-1.9933
508	D	A	-2.1761
509	I	A	0.0000
510	D	A	-2.8475
511	K	A	-2.8421
512	I	A	0.0000
513	G	A	-1.3035
514	Y	A	-0.9495
515	P	A	0.0000
516	L	A	-1.7192
517	D	A	-2.8629
518	I	A	0.0000
519	R	A	-2.9028
520	E	A	-3.3570
521	Y	A	-2.3379
522	D	A	-2.4587
523	Y	A	-0.9903
524	T	A	-0.6613
525	P	A	-0.7331
526	V	A	-0.6933
527	D	A	-1.2759
528	A	A	-0.2322
529	G	A	0.3410
530	I	A	1.0897
531	Y	A	-0.0660
532	D	A	-1.7576
533	I	A	-1.0181
534	P	A	-1.2968
535	Q	A	-0.7645
536	L	A	0.0000
537	K	A	-1.8382
538	S	A	-0.7998
539	I	A	-0.6995
540	S	A	0.0000
541	K	A	-2.0593
542	G	A	-1.0337
543	P	A	-0.7764
544	W	A	0.0000
545	E	A	-0.5660
546	L	A	0.0000
547	I	A	0.7220
548	T	A	0.1876
549	T	A	0.0000
550	P	A	-0.3372
551	D	A	0.0000
552	G	A	-0.2892
553	Y	A	0.0000
554	Y	A	0.0000
555	Q	A	0.0000
556	M	A	0.0000
557	K	A	-0.7583
558	D	A	0.0000
559	T	A	0.1756
560	V	A	1.0965
561	S	A	0.1714
562	G	A	-0.4757
563	K	A	-1.0293
564	C	A	0.0000
565	L	A	0.0000
566	A	A	0.0000
567	L	A	0.0000
568	F	A	-0.3833
569	T	A	-0.6464
570	G	A	-0.9919
571	S	A	-1.3909
572	K	A	-2.3169
573	H	A	-1.5090
574	L	A	-1.1068
575	D	A	-1.9896
576	V	A	0.0000
577	V	A	0.0000
578	T	A	0.0000
579	Q	A	-1.1153
580	V	A	0.0000
581	G	A	0.0000
582	A	A	0.0000
583	R	A	-1.7999
584	P	A	0.0000
585	E	A	-0.6085
586	L	A	-0.4943
587	R	A	-1.2133
588	N	A	-1.8188
589	C	A	-1.1184
590	A	A	-1.3524
591	D	A	-1.9541
592	V	A	0.0000
593	S	A	-0.6253
594	V	A	-0.9940
595	G	A	-1.5218
596	Q	A	-1.8470
597	D	A	-2.7867
598	Q	A	-2.5873
599	R	A	-2.3425
600	N	A	-2.2770
601	T	A	-1.8705
602	A	A	0.0000
603	N	A	-1.0717
604	E	A	-1.3847
605	R	A	0.0000
606	N	A	0.0000
607	T	A	0.0000
608	Q	A	0.0000
609	K	A	0.0000
610	W	A	0.0000
611	Q	A	0.0000
612	I	A	0.0000
613	R	A	-1.0625
614	A	A	-2.1571
615	D	A	-3.8575
616	K	A	-4.0082
617	D	A	-4.0841
618	G	A	-2.9643
619	K	A	-2.7714
620	Y	A	-0.6787
621	T	A	-0.3133
622	I	A	0.0000
623	S	A	0.0000
624	P	A	0.0000
625	A	A	0.0000
626	L	A	0.0000
627	T	A	0.0000
628	Q	A	0.0000
629	Q	A	0.0000
630	R	A	-0.4158
631	L	A	0.0000
632	A	A	0.2464
633	I	A	0.9195
634	A	A	0.0000
635	T	A	-0.4335
636	G	A	-1.0845
637	N	A	-1.6513
638	E	A	0.0000
639	Q	A	-1.8956
640	N	A	-1.5868
641	I	A	-1.4302
642	D	A	-1.5683
643	L	A	0.0000
644	E	A	-2.5260
645	T	A	-1.6611
646	H	A	-1.9451
647	R	A	-1.8492
648	P	A	-0.9016
649	A	A	-0.4620
650	A	A	-0.3124
651	G	A	-0.1773
652	T	A	-0.3172
653	V	A	0.0000
654	A	A	0.0000
655	Q	A	0.0000
656	F	A	0.0000
657	P	A	0.0000
658	A	A	-0.3216
659	D	A	-0.8756
660	L	A	-0.4646
661	V	A	0.1082
662	S	A	-0.1818