Chain sequence(s) |
A: MRSGSHHHHHHRSDITSLYKKAGSAAAPFTMENLYFQSYQGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP |
Distance of aggregation | 10 Å |
Dynamic mode | No |
Mutated residues | IA73D, IA141K, LA312K |
Energy difference between WT (input) and mutated protein (by FoldX) | 0.0917701 kcal/mol
Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
residue index | residue name | chain | Aggrescan3D score | mutation |
---|---|---|---|---|
residue index | residue name | chain | Aggrescan3D score | |
1 | M | A | -0.0801 | |
2 | R | A | -1.5302 | |
3 | S | A | -0.6471 | |
4 | G | A | -0.5188 | |
5 | S | A | -0.3363 | |
6 | H | A | 0.0000 | |
7 | H | A | -0.5484 | |
8 | H | A | -0.7122 | |
9 | H | A | -1.1304 | |
10 | H | A | -1.8419 | |
11 | H | A | -1.6278 | |
12 | R | A | -1.7657 | |
13 | S | A | 0.0000 | |
14 | D | A | 0.0000 | |
15 | I | A | 0.8741 | |
16 | T | A | 0.0586 | |
17 | S | A | 0.1158 | |
18 | L | A | 0.2267 | |
19 | Y | A | 0.0000 | |
20 | K | A | -1.5254 | |
21 | K | A | -2.2763 | |
22 | A | A | -1.2246 | |
23 | G | A | -1.3128 | |
24 | S | A | -0.8783 | |
25 | A | A | 0.0000 | |
26 | A | A | 0.0121 | |
27 | A | A | 0.4178 | |
28 | P | A | -0.0139 | |
29 | F | A | 0.0000 | |
30 | T | A | -0.6209 | |
31 | M | A | -0.5233 | |
32 | E | A | -1.9851 | |
33 | N | A | -1.3345 | |
34 | L | A | 0.1384 | |
35 | Y | A | 0.8278 | |
36 | F | A | 0.1356 | |
37 | Q | A | -0.9128 | |
38 | S | A | -1.0304 | |
39 | Y | A | 0.0000 | |
40 | Q | A | 0.0000 | |
41 | G | A | -0.6842 | |
42 | N | A | -0.9181 | |
43 | S | A | 0.0000 | |
44 | D | A | -0.3436 | |
45 | C | A | 0.3601 | |
46 | Y | A | 1.0782 | |
47 | F | A | 0.0000 | |
48 | G | A | 0.0000 | |
49 | N | A | 0.0000 | |
50 | G | A | 0.0000 | |
51 | S | A | 0.0000 | |
52 | A | A | -0.9921 | |
53 | Y | A | 0.0000 | |
54 | R | A | -0.8042 | |
55 | G | A | -0.6622 | |
56 | T | A | -0.3871 | |
57 | H | A | -0.2309 | |
58 | S | A | -0.1047 | |
59 | L | A | -0.2612 | |
60 | T | A | 0.0000 | |
61 | E | A | -2.2301 | |
62 | S | A | -1.3951 | |
63 | G | A | -1.1678 | |
64 | A | A | -0.8160 | |
65 | S | A | -0.2672 | |
66 | C | A | 0.0000 | |
67 | L | A | -0.0037 | |
68 | P | A | -0.8710 | |
69 | W | A | 0.0000 | |
70 | N | A | -1.7034 | |
71 | S | A | -0.7517 | |
72 | M | A | -0.1827 | |
73 | D | A | -1.7311 | mutated: IA73D |
74 | L | A | 0.0000 | |
75 | I | A | -0.1836 | |
76 | G | A | -0.7240 | |
77 | K | A | -1.4858 | |
78 | V | A | -0.0653 | |
79 | Y | A | 0.9141 | |
80 | T | A | 0.3823 | |
81 | A | A | 0.0215 | |
82 | Q | A | -0.2487 | |
83 | N | A | -0.4271 | |
84 | P | A | -0.5135 | |
85 | S | A | -0.9423 | |
86 | A | A | 0.0000 | |
87 | Q | A | 0.0000 | |
88 | A | A | 0.0000 | |
89 | L | A | 0.0000 | |
90 | G | A | 0.0000 | |
91 | L | A | 0.0000 | |
92 | G | A | -1.5609 | |
93 | K | A | -2.3807 | |
94 | H | A | -1.3857 | |
95 | N | A | 0.0000 | |
96 | Y | A | -0.2161 | |
97 | C | A | 0.0000 | |
98 | R | A | 0.0000 | |
99 | N | A | 0.0000 | |
100 | P | A | -1.1540 | |
101 | D | A | -2.0428 | |
102 | G | A | -1.6743 | |
103 | D | A | -1.3116 | |
104 | A | A | -0.8229 | |
105 | K | A | -1.0038 | |
106 | P | A | 0.0000 | |
107 | W | A | -0.2377 | |
108 | C | A | 0.0000 | |
109 | H | A | 0.0000 | |
110 | V | A | 0.0000 | |
111 | L | A | -1.2302 | |
112 | K | A | -2.7673 | |
113 | N | A | -3.1739 | |
114 | R | A | -3.5244 | |
115 | R | A | -3.0731 | |
116 | L | A | -1.1998 | |
117 | T | A | -0.7271 | |
118 | W | A | 0.0223 | |
119 | E | A | -0.8701 | |
120 | Y | A | -0.4205 | |
121 | C | A | 0.0000 | |
122 | D | A | -1.4907 | |
123 | V | A | 0.0000 | |
124 | P | A | -0.2602 | |
125 | S | A | -0.1200 | |
126 | C | A | 0.0649 | |
127 | S | A | 0.0000 | |
128 | T | A | 0.0000 | |
129 | C | A | 0.0000 | |
130 | G | A | -0.1010 | |
131 | L | A | 0.2585 | |
132 | R | A | 0.0000 | |
133 | Q | A | -0.5890 | |
134 | Y | A | 0.4606 | |
135 | S | A | -0.1510 | |
136 | Q | A | -0.9051 | |
137 | P | A | -1.5109 | |
138 | Q | A | -2.0453 | |
139 | F | A | -1.8567 | |
140 | R | A | -3.0511 | |
141 | K | A | -2.7579 | mutated: IA141K |
142 | K | A | -1.3541 | |
143 | G | A | -0.9307 | |
144 | G | A | -0.1518 | |
145 | L | A | 1.2651 | |
146 | F | A | 0.9010 | |
147 | A | A | -0.2421 | |
148 | D | A | -1.3183 | |
149 | I | A | 0.0000 | |
150 | A | A | -0.3971 | |
151 | S | A | 0.0000 | |
152 | H | A | 0.0000 | |
153 | P | A | 0.0000 | |
154 | W | A | 0.0000 | |
155 | Q | A | 0.0000 | |
156 | A | A | 0.0000 | |
157 | A | A | 0.0000 | |
158 | I | A | 0.0000 | |
159 | F | A | 0.0000 | |
160 | A | A | 0.0000 | |
161 | K | A | -2.6438 | |
162 | H | A | -2.7522 | |
163 | R | A | -3.3169 | |
164 | R | A | -3.6246 | |
165 | S | A | -2.0242 | |
166 | P | A | -1.5896 | |
167 | G | A | -2.1892 | |
168 | E | A | -2.7234 | |
169 | R | A | -2.3218 | |
170 | F | A | -0.8301 | |
171 | L | A | -0.0860 | |
172 | C | A | 0.0000 | |
173 | G | A | 0.0000 | |
174 | G | A | 0.0000 | |
175 | I | A | 0.0000 | |
176 | L | A | 0.0000 | |
177 | I | A | 0.0000 | |
178 | S | A | -0.7496 | |
179 | S | A | -1.2417 | |
180 | C | A | 0.0000 | |
181 | W | A | 0.0000 | |
182 | I | A | 0.0000 | |
183 | L | A | 0.0000 | |
184 | S | A | 0.0000 | |
185 | A | A | 0.0000 | |
186 | A | A | 0.0000 | |
187 | H | A | -1.4035 | |
188 | C | A | -0.4999 | |
189 | F | A | 0.0000 | |
190 | Q | A | 0.0000 | |
191 | E | A | -2.6280 | |
192 | R | A | -2.6091 | |
193 | F | A | -1.6890 | |
194 | P | A | -1.4615 | |
195 | P | A | -1.7949 | |
196 | H | A | -1.9341 | |
197 | H | A | -2.0773 | |
198 | L | A | 0.0000 | |
199 | T | A | 0.0000 | |
200 | V | A | 0.0000 | |
201 | I | A | 0.0000 | |
202 | L | A | -0.3045 | |
203 | G | A | 0.0000 | |
204 | R | A | -0.1018 | |
205 | T | A | 0.1948 | |
206 | Y | A | 1.1882 | |
207 | R | A | 0.8430 | |
208 | V | A | 2.0029 | |
209 | V | A | 1.5282 | |
210 | P | A | 0.4976 | |
211 | G | A | -1.0551 | |
212 | E | A | -2.1475 | |
213 | E | A | -1.9116 | |
214 | E | A | -2.6941 | |
215 | Q | A | -2.4761 | |
216 | K | A | -2.3560 | |
217 | F | A | 0.0000 | |
218 | E | A | -3.2570 | |
219 | V | A | 0.0000 | |
220 | E | A | -2.4687 | |
221 | K | A | -1.8832 | |
222 | Y | A | -0.5026 | |
223 | I | A | -0.4040 | |
224 | V | A | -0.6664 | |
225 | H | A | -1.4535 | |
226 | K | A | -2.6192 | |
227 | E | A | -2.7388 | |
228 | F | A | -2.1423 | |
229 | D | A | -2.5413 | |
230 | D | A | -3.1157 | |
231 | D | A | -2.9960 | |
232 | T | A | -1.8051 | |
233 | Y | A | 0.0000 | |
234 | D | A | -1.9105 | |
235 | N | A | -1.7810 | |
236 | D | A | 0.0000 | |
237 | I | A | 0.0000 | |
238 | A | A | 0.0000 | |
239 | L | A | 0.0000 | |
240 | L | A | 0.0000 | |
241 | Q | A | 0.0000 | |
242 | L | A | 0.0000 | |
243 | K | A | -2.8425 | |
244 | S | A | -2.4056 | |
245 | D | A | -3.3919 | |
246 | S | A | 0.0000 | |
247 | S | A | 0.0000 | |
248 | R | A | -2.3825 | |
249 | C | A | -1.7205 | |
250 | A | A | -1.3093 | |
251 | Q | A | -2.2575 | |
252 | E | A | -2.0893 | |
253 | S | A | -1.5356 | |
254 | S | A | 0.0000 | |
255 | V | A | 0.4958 | |
256 | V | A | 0.0000 | |
257 | R | A | -0.8554 | |
258 | T | A | 0.0000 | |
259 | V | A | 0.0000 | |
260 | C | A | 0.3626 | |
261 | L | A | 0.1985 | |
262 | P | A | 0.0000 | |
263 | P | A | -0.6558 | |
264 | A | A | -1.1904 | |
265 | D | A | -2.1339 | |
266 | L | A | -1.0342 | |
267 | Q | A | -1.4717 | |
268 | L | A | 0.0000 | |
269 | P | A | -1.6963 | |
270 | D | A | -2.1863 | |
271 | W | A | -0.9606 | |
272 | T | A | -0.7262 | |
273 | E | A | -1.4978 | |
274 | C | A | 0.0000 | |
275 | E | A | 0.0000 | |
276 | L | A | 0.0000 | |
277 | S | A | 0.0000 | |
278 | G | A | 0.0000 | |
279 | Y | A | 0.0000 | |
280 | G | A | 0.0000 | |
281 | K | A | -0.4668 | |
282 | H | A | -0.4664 | |
283 | E | A | -1.5949 | |
284 | A | A | -0.0694 | |
285 | L | A | 1.1377 | |
286 | S | A | 0.4978 | |
287 | P | A | 0.9238 | |
288 | F | A | 1.7553 | |
289 | Y | A | 0.6779 | |
290 | S | A | 0.5060 | |
291 | E | A | 0.0000 | |
292 | R | A | -0.1622 | |
293 | L | A | 0.0000 | |
294 | K | A | 0.0693 | |
295 | E | A | 0.0000 | |
296 | A | A | 0.0000 | |
297 | H | A | -1.3337 | |
298 | V | A | 0.0000 | |
299 | R | A | -1.1426 | |
300 | L | A | 0.0000 | |
301 | Y | A | 0.0000 | |
302 | P | A | -1.0297 | |
303 | S | A | -1.2098 | |
304 | S | A | -1.2748 | |
305 | R | A | -2.4146 | |
306 | C | A | 0.0000 | |
307 | T | A | -1.2899 | |
308 | S | A | -1.3229 | |
309 | Q | A | 0.0000 | |
310 | H | A | 0.0000 | |
311 | L | A | -0.9728 | |
312 | K | A | -1.7426 | mutated: LA312K |
313 | N | A | -1.6549 | |
314 | R | A | -1.4998 | |
315 | T | A | -0.4792 | |
316 | V | A | 0.6830 | |
317 | T | A | -0.3238 | |
318 | D | A | -1.4673 | |
319 | N | A | 0.0000 | |
320 | M | A | 0.0000 | |
321 | L | A | -0.1328 | |
322 | C | A | 0.0000 | |
323 | A | A | 0.0000 | |
324 | G | A | 0.0000 | |
325 | D | A | -2.0262 | |
326 | T | A | -1.2353 | |
327 | R | A | 0.0000 | |
328 | S | A | -1.2762 | |
329 | G | A | -1.9046 | |
330 | G | A | -1.7877 | |
331 | P | A | -1.4906 | |
332 | Q | A | -1.8729 | |
333 | A | A | -0.9812 | |
334 | N | A | -0.5651 | |
335 | L | A | 0.6905 | |
336 | H | A | -0.0492 | |
337 | D | A | -0.2469 | |
338 | A | A | -0.6229 | |
339 | C | A | -0.6062 | |
340 | Q | A | 0.0000 | |
341 | G | A | 0.0000 | |
342 | D | A | 0.0000 | |
343 | S | A | -0.0893 | |
344 | G | A | 0.0000 | |
345 | G | A | 0.0000 | |
346 | P | A | 0.0000 | |
347 | L | A | 0.0000 | |
348 | V | A | 0.0000 | |
349 | C | A | 0.0000 | |
350 | L | A | -1.2724 | |
351 | N | A | 0.0000 | |
352 | D | A | -2.5428 | |
353 | G | A | -1.8136 | |
354 | R | A | -1.4655 | |
355 | M | A | 0.0000 | |
356 | T | A | 0.0000 | |
357 | L | A | 0.0000 | |
358 | V | A | -0.0045 | |
359 | G | A | 0.0000 | |
360 | I | A | 0.0000 | |
361 | I | A | 0.0000 | |
362 | S | A | -0.3167 | |
363 | W | A | -0.1757 | |
364 | G | A | -0.3200 | |
365 | L | A | 0.3651 | |
366 | G | A | -0.3553 | |
367 | C | A | 0.0000 | |
368 | G | A | -1.2194 | |
369 | Q | A | -1.7692 | |
370 | K | A | -1.2473 | |
371 | D | A | -1.7846 | |
372 | V | A | 0.0000 | |
373 | P | A | 0.0000 | |
374 | G | A | 0.0000 | |
375 | V | A | 0.0000 | |
376 | Y | A | 0.0000 | |
377 | T | A | 0.0000 | |
378 | K | A | -0.4019 | |
379 | V | A | 0.0000 | |
380 | T | A | 0.0000 | |
381 | N | A | 0.0000 | |
382 | Y | A | 0.0000 | |
383 | L | A | 0.0000 | |
384 | D | A | -1.5127 | |
385 | W | A | 0.0000 | |
386 | I | A | 0.0000 | |
387 | R | A | -2.9048 | |
388 | D | A | -3.1396 | |
389 | N | A | -2.1021 | |
390 | M | A | 0.0000 | |
391 | R | A | -2.8495 |