| Chain sequence(s) |
A: MDTARIAVVGAGVVGLSTAVCISKLVPRCSVTIISDKFTPDTTSDVAAGMLIPHTYPDTPIHTQKQWFRETFNHLFAIANSAEAGDAGVHLVSGWQIFQSTPTEEVPFWADVVLGFRKMTEAELKKFPQYVFGQAFTTLKCECPAYLPWLEKRIKGSGGWTLTRRIEDLWELHPSFDIVVNCSGLGSRQLAGDSKIFPVRGQVLQVQAPWVEHFIRDGSGLTYIYPGTSHVTLGGTRQKGDWNLSPDAENSREILSRCCALEPSLHGACNIREKVGLRPYRPGVRLQTELLARDGQRLPVVHHYGHGSGGISVHWGTALEAARLVSECVHALRTPIPKSNL |
| Distance of aggregation | 10 Å |
| Dynamic mode | No |
| Mutated residues | WA160K, LA114V, LA244V |
| Energy difference between WT (input) and mutated protein (by FoldX) | 3.55503 kcal/mol
CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 1 | M | A | 0.6612 | |
| 2 | D | A | -0.3537 | |
| 3 | T | A | -0.4826 | |
| 4 | A | A | 0.0000 | |
| 5 | R | A | -0.8444 | |
| 6 | I | A | 0.0000 | |
| 7 | A | A | 0.0000 | |
| 8 | V | A | 0.0000 | |
| 9 | V | A | 0.0000 | |
| 10 | G | A | -0.8239 | |
| 11 | A | A | 0.0000 | |
| 12 | G | A | -0.5260 | |
| 13 | V | A | 0.0000 | |
| 14 | V | A | 0.1664 | |
| 15 | G | A | 0.0000 | |
| 16 | L | A | 0.0000 | |
| 17 | S | A | 0.0000 | |
| 18 | T | A | 0.0000 | |
| 19 | A | A | 0.0000 | |
| 20 | V | A | 0.0000 | |
| 21 | C | A | 0.0000 | |
| 22 | I | A | 0.0000 | |
| 23 | S | A | 0.0000 | |
| 24 | K | A | -1.7561 | |
| 25 | L | A | 0.0000 | |
| 26 | V | A | 0.0000 | |
| 27 | P | A | -1.5815 | |
| 28 | R | A | -2.1117 | |
| 29 | C | A | -1.2289 | |
| 30 | S | A | -0.8027 | |
| 31 | V | A | 0.0000 | |
| 32 | T | A | -0.5240 | |
| 33 | I | A | 0.0000 | |
| 34 | I | A | 0.0000 | |
| 35 | S | A | 0.0000 | |
| 36 | D | A | -3.3920 | |
| 37 | K | A | -3.1824 | |
| 38 | F | A | -1.5988 | |
| 39 | T | A | -1.1208 | |
| 40 | P | A | -1.3437 | |
| 41 | D | A | -2.4885 | |
| 42 | T | A | -1.5249 | |
| 43 | T | A | -0.6840 | |
| 44 | S | A | -0.3845 | |
| 45 | D | A | 0.0000 | |
| 46 | V | A | -0.3805 | |
| 47 | A | A | -0.0586 | |
| 48 | A | A | 0.0227 | |
| 49 | G | A | 0.0000 | |
| 50 | M | A | -0.0002 | |
| 51 | L | A | 0.0000 | |
| 52 | I | A | 0.0000 | |
| 53 | P | A | 0.0000 | |
| 54 | H | A | -0.1142 | |
| 55 | T | A | 0.0257 | |
| 56 | Y | A | 0.4263 | |
| 57 | P | A | -0.7533 | |
| 58 | D | A | -1.8404 | |
| 59 | T | A | -0.9072 | |
| 60 | P | A | -0.8993 | |
| 61 | I | A | -0.9857 | |
| 62 | H | A | -1.8501 | |
| 63 | T | A | -1.4467 | |
| 64 | Q | A | 0.0000 | |
| 65 | K | A | -2.0216 | |
| 66 | Q | A | -2.4031 | |
| 67 | W | A | 0.0000 | |
| 68 | F | A | 0.0000 | |
| 69 | R | A | -3.2540 | |
| 70 | E | A | -2.6476 | |
| 71 | T | A | 0.0000 | |
| 72 | F | A | 0.0000 | |
| 73 | N | A | -1.5963 | |
| 74 | H | A | -0.9398 | |
| 75 | L | A | 0.0000 | |
| 76 | F | A | -0.0296 | |
| 77 | A | A | -0.2196 | |
| 78 | I | A | -0.3628 | |
| 79 | A | A | 0.0000 | |
| 80 | N | A | -1.3192 | |
| 81 | S | A | -0.7729 | |
| 82 | A | A | -0.8837 | |
| 83 | E | A | -1.4136 | |
| 84 | A | A | -1.3581 | |
| 85 | G | A | -1.7784 | |
| 86 | D | A | -2.0320 | |
| 87 | A | A | 0.0000 | |
| 88 | G | A | 0.0000 | |
| 89 | V | A | 0.0000 | |
| 90 | H | A | -0.9441 | |
| 91 | L | A | -0.0232 | |
| 92 | V | A | 0.0000 | |
| 93 | S | A | -0.2718 | |
| 94 | G | A | 0.0000 | |
| 95 | W | A | -0.2101 | |
| 96 | Q | A | 0.0000 | |
| 97 | I | A | 0.0000 | |
| 98 | F | A | -0.0238 | |
| 99 | Q | A | -0.6802 | |
| 100 | S | A | -0.1527 | |
| 101 | T | A | -0.1099 | |
| 102 | P | A | -0.5708 | |
| 103 | T | A | -0.6266 | |
| 104 | E | A | -1.1016 | |
| 105 | E | A | 0.0000 | |
| 106 | V | A | -0.3579 | |
| 107 | P | A | -0.3933 | |
| 108 | F | A | -0.8048 | |
| 109 | W | A | 0.0000 | |
| 110 | A | A | -0.5711 | |
| 111 | D | A | -1.6240 | |
| 112 | V | A | -1.3080 | |
| 113 | V | A | 0.0000 | |
| 114 | V | A | 0.6087 | mutated: LA114V |
| 115 | G | A | -0.2758 | |
| 116 | F | A | -0.3734 | |
| 117 | R | A | -0.9142 | |
| 118 | K | A | -1.0097 | |
| 119 | M | A | 0.0000 | |
| 120 | T | A | -1.4075 | |
| 121 | E | A | -2.2911 | |
| 122 | A | A | -1.9836 | |
| 123 | E | A | -1.9949 | |
| 124 | L | A | 0.0000 | |
| 125 | K | A | -2.9790 | |
| 126 | K | A | -3.0625 | |
| 127 | F | A | 0.0000 | |
| 128 | P | A | -1.3798 | |
| 129 | Q | A | -1.1495 | |
| 130 | Y | A | 0.0000 | |
| 131 | V | A | 0.7945 | |
| 132 | F | A | 0.3584 | |
| 133 | G | A | 0.0000 | |
| 134 | Q | A | 0.0000 | |
| 135 | A | A | 0.0000 | |
| 136 | F | A | 0.0000 | |
| 137 | T | A | 0.3058 | |
| 138 | T | A | 0.0000 | |
| 139 | L | A | 0.0000 | |
| 140 | K | A | 0.0000 | |
| 141 | C | A | 0.0000 | |
| 142 | E | A | -0.6658 | |
| 143 | C | A | 0.0000 | |
| 144 | P | A | -0.5105 | |
| 145 | A | A | -0.5867 | |
| 146 | Y | A | 0.0000 | |
| 147 | L | A | 0.0000 | |
| 148 | P | A | -0.7750 | |
| 149 | W | A | -1.0686 | |
| 150 | L | A | 0.0000 | |
| 151 | E | A | -1.5690 | |
| 152 | K | A | -2.1403 | |
| 153 | R | A | -1.9323 | |
| 154 | I | A | 0.0000 | |
| 155 | K | A | -2.4024 | |
| 156 | G | A | -1.8735 | |
| 157 | S | A | -1.7027 | |
| 158 | G | A | -1.4424 | |
| 159 | G | A | 0.0000 | |
| 160 | K | A | -1.9038 | mutated: WA160K |
| 161 | T | A | -0.6914 | |
| 162 | L | A | -0.2749 | |
| 163 | T | A | -1.1813 | |
| 164 | R | A | -2.3874 | |
| 165 | R | A | -3.3103 | |
| 166 | I | A | 0.0000 | |
| 167 | E | A | -3.1091 | |
| 168 | D | A | -2.5328 | |
| 169 | L | A | 0.0000 | |
| 170 | W | A | -1.2440 | |
| 171 | E | A | -1.5980 | |
| 172 | L | A | 0.0000 | |
| 173 | H | A | 0.0000 | |
| 174 | P | A | -0.7326 | |
| 175 | S | A | -0.6641 | |
| 176 | F | A | -0.8644 | |
| 177 | D | A | -1.6576 | |
| 178 | I | A | 0.0000 | |
| 179 | V | A | 0.0000 | |
| 180 | V | A | 0.0000 | |
| 181 | N | A | 0.0000 | |
| 182 | C | A | 0.0000 | |
| 183 | S | A | -0.4601 | |
| 184 | G | A | -0.0861 | |
| 185 | L | A | 0.0766 | |
| 186 | G | A | -0.6352 | |
| 187 | S | A | 0.0000 | |
| 188 | R | A | -1.4410 | |
| 189 | Q | A | -1.7067 | |
| 190 | L | A | -1.3610 | |
| 191 | A | A | -1.1096 | |
| 192 | G | A | -1.2181 | |
| 193 | D | A | -1.2657 | |
| 194 | S | A | -1.0982 | |
| 195 | K | A | -1.5012 | |
| 196 | I | A | 0.0000 | |
| 197 | F | A | -0.0380 | |
| 198 | P | A | 0.0000 | |
| 199 | V | A | 0.0000 | |
| 200 | R | A | 0.0000 | |
| 201 | G | A | 0.0000 | |
| 202 | Q | A | 0.0000 | |
| 203 | V | A | -0.4212 | |
| 204 | L | A | 0.0000 | |
| 205 | Q | A | -1.6346 | |
| 206 | V | A | 0.0000 | |
| 207 | Q | A | -1.2769 | |
| 208 | A | A | 0.0000 | |
| 209 | P | A | -0.4456 | |
| 210 | W | A | 0.3029 | |
| 211 | V | A | -0.5696 | |
| 212 | E | A | -1.6702 | |
| 213 | H | A | -0.9978 | |
| 214 | F | A | 0.0000 | |
| 215 | I | A | 0.0000 | |
| 216 | R | A | -0.3022 | |
| 217 | D | A | -0.4467 | |
| 218 | G | A | -0.6042 | |
| 219 | S | A | -0.9104 | |
| 220 | G | A | -0.8684 | |
| 221 | L | A | -0.2123 | |
| 222 | T | A | 0.0000 | |
| 223 | Y | A | -0.0474 | |
| 224 | I | A | 0.0000 | |
| 225 | Y | A | 0.0000 | |
| 226 | P | A | 0.0000 | |
| 227 | G | A | -0.9319 | |
| 228 | T | A | -0.8495 | |
| 229 | S | A | -0.6992 | |
| 230 | H | A | -1.0450 | |
| 231 | V | A | 0.0000 | |
| 232 | T | A | -0.7253 | |
| 233 | L | A | 0.0000 | |
| 234 | G | A | 0.0000 | |
| 235 | G | A | 0.0000 | |
| 236 | T | A | -1.3872 | |
| 237 | R | A | -2.8405 | |
| 238 | Q | A | -2.6668 | |
| 239 | K | A | -2.4286 | |
| 240 | G | A | -1.3478 | |
| 241 | D | A | -0.9748 | |
| 242 | W | A | 0.4243 | |
| 243 | N | A | -0.0237 | |
| 244 | V | A | 1.2505 | mutated: LA244V |
| 245 | S | A | 0.1323 | |
| 246 | P | A | -0.8528 | |
| 247 | D | A | -1.8345 | |
| 248 | A | A | -1.9701 | |
| 249 | E | A | -2.9999 | |
| 250 | N | A | 0.0000 | |
| 251 | S | A | -2.1957 | |
| 252 | R | A | -3.1378 | |
| 253 | E | A | -2.5659 | |
| 254 | I | A | 0.0000 | |
| 255 | L | A | -1.2795 | |
| 256 | S | A | -1.2854 | |
| 257 | R | A | -0.8725 | |
| 258 | C | A | 0.0000 | |
| 259 | C | A | -0.6730 | |
| 260 | A | A | -0.5166 | |
| 261 | L | A | 0.0000 | |
| 262 | E | A | -0.8827 | |
| 263 | P | A | -0.8522 | |
| 264 | S | A | -0.5928 | |
| 265 | L | A | 0.0000 | |
| 266 | H | A | -1.3456 | |
| 267 | G | A | -1.0773 | |
| 268 | A | A | -0.7358 | |
| 269 | C | A | -0.6370 | |
| 270 | N | A | -1.7856 | |
| 271 | I | A | -1.3889 | |
| 272 | R | A | -2.4894 | |
| 273 | E | A | -1.9401 | |
| 274 | K | A | -1.0865 | |
| 275 | V | A | -0.0159 | |
| 276 | G | A | -0.1098 | |
| 277 | L | A | 0.0000 | |
| 278 | R | A | -0.6095 | |
| 279 | P | A | 0.0570 | |
| 280 | Y | A | 0.0635 | |
| 281 | R | A | -0.4675 | |
| 282 | P | A | -0.5279 | |
| 283 | G | A | -0.4252 | |
| 284 | V | A | 0.0000 | |
| 285 | R | A | -0.6945 | |
| 286 | L | A | -0.5825 | |
| 287 | Q | A | -1.1686 | |
| 288 | T | A | -0.7661 | |
| 289 | E | A | -0.5221 | |
| 290 | L | A | 0.3292 | |
| 291 | L | A | -0.0485 | |
| 292 | A | A | -1.4253 | |
| 293 | R | A | -2.5432 | |
| 294 | D | A | -3.0843 | |
| 295 | G | A | -2.5263 | |
| 296 | Q | A | -2.9808 | |
| 297 | R | A | -2.5723 | |
| 298 | L | A | 0.0000 | |
| 299 | P | A | 0.0000 | |
| 300 | V | A | 0.0000 | |
| 301 | V | A | 0.0000 | |
| 302 | H | A | 0.0000 | |
| 303 | H | A | 0.0000 | |
| 304 | Y | A | 0.0000 | |
| 305 | G | A | 0.0000 | |
| 306 | H | A | 0.0000 | |
| 307 | G | A | -0.0106 | |
| 308 | S | A | -0.5862 | |
| 309 | G | A | -0.2536 | |
| 310 | G | A | -0.1699 | |
| 311 | I | A | 0.0000 | |
| 312 | S | A | 0.0000 | |
| 313 | V | A | 0.0000 | |
| 314 | H | A | 0.0000 | |
| 315 | W | A | -0.4278 | |
| 316 | G | A | 0.0000 | |
| 317 | T | A | 0.0000 | |
| 318 | A | A | 0.0000 | |
| 319 | L | A | -0.2158 | |
| 320 | E | A | -0.8435 | |
| 321 | A | A | 0.0000 | |
| 322 | A | A | 0.0000 | |
| 323 | R | A | -1.5413 | |
| 324 | L | A | -1.1808 | |
| 325 | V | A | 0.0000 | |
| 326 | S | A | 0.0000 | |
| 327 | E | A | -2.2494 | |
| 328 | C | A | -1.3640 | |
| 329 | V | A | 0.0000 | |
| 330 | H | A | -1.9668 | |
| 331 | A | A | -1.2314 | |
| 332 | L | A | -0.9565 | |
| 333 | R | A | -2.0748 | |
| 334 | T | A | -1.1968 | |
| 335 | P | A | -1.2132 | |
| 336 | I | A | -0.7089 | |
| 337 | P | A | -0.9680 | |
| 338 | K | A | -1.2169 | |
| 339 | S | A | -1.0078 | |
| 340 | N | A | -1.0839 | |
| 341 | L | A | -0.0270 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine