Project name: C226S

Chain sequence(s)	H: EVQLVESGGGLVQPGGSLRLSCAASGFAFSTYDMSWVRQAPGKGLEWVATISSGGSYTYYLDSVKGRFTISRDSSKNTLYLQMNSLRAEDTAVYYCAPTTVVPFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTSAA; L: DIQMTQSPSSLSASVGDRVTITCKASQNVRTVVAWYQQKPGKAPKTLIYLASNRHTGVPSRFSGSGSGTDFTLTISSLQPEDFATYFCLQHWSYPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
Distance of aggregation	10 Å
Dynamic mode	No

• Minimal score value: -3.5951
• Maximal score value: 1.3161
• Average score: -0.7242
• Total score value: -320.0755

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	D	L	-2.4112
2	I	L	0.0000
3	Q	L	-2.0430
4	M	L	0.0000
5	T	L	-1.1073
6	Q	L	0.0000
7	S	L	-0.6126
8	P	L	-0.5733
9	S	L	-0.8643
10	S	L	-0.7463
11	L	L	-0.5151
12	S	L	-0.9085
13	A	L	0.0000
14	S	L	-0.5445
15	V	L	0.4444
16	G	L	-0.5811
17	D	L	-1.5477
18	R	L	-2.2353
19	V	L	0.0000
20	T	L	-0.5898
21	I	L	0.0000
22	T	L	-0.7526
23	C	L	0.0000
24	K	L	-2.1091
25	A	L	-1.9782
26	S	L	-2.0830
27	Q	L	-2.6910
28	N	L	-2.7187
29	V	L	0.0000
30	R	L	-2.5033
31	T	L	-1.2381
32	V	L	0.0000
33	V	L	0.0000
34	A	L	0.0000
35	W	L	0.0000
36	Y	L	0.0000
37	Q	L	0.0000
38	Q	L	-1.3835
39	K	L	-1.8852
40	P	L	-1.7849
41	G	L	-1.5393
42	K	L	-2.5277
43	A	L	-1.7690
44	P	L	0.0000
45	K	L	-1.8834
46	T	L	0.0000
47	L	L	0.0000
48	I	L	0.0000
49	Y	L	0.0085
50	L	L	0.0664
51	A	L	0.0000
52	S	L	-0.9319
53	N	L	-1.3438
54	R	L	-2.2070
55	H	L	0.0000
56	T	L	-1.1718
57	G	L	-1.3335
58	V	L	0.0000
59	P	L	-0.7377
60	S	L	-0.8743
61	R	L	-1.0620
62	F	L	0.0000
63	S	L	-0.9385
64	G	L	0.0000
65	S	L	-0.6834
66	G	L	-1.2532
67	S	L	-1.7067
68	G	L	-2.2629
69	T	L	-2.3545
70	D	L	-2.6651
71	F	L	0.0000
72	T	L	-0.7516
73	L	L	0.0000
74	T	L	-0.6328
75	I	L	0.0000
76	S	L	-1.2897
77	S	L	-1.0780
78	L	L	0.0000
79	Q	L	-0.9906
80	P	L	-0.9775
81	E	L	-1.9785
82	D	L	0.0000
83	F	L	-0.6501
84	A	L	0.0000
85	T	L	-0.9370
86	Y	L	0.0000
87	F	L	0.0000
88	C	L	0.0000
89	L	L	0.0000
90	Q	L	0.0000
91	H	L	0.4323
92	W	L	0.1620
93	S	L	0.0490
94	Y	L	0.2286
95	P	L	-0.3519
96	L	L	0.0000
97	T	L	-0.7133
98	F	L	0.0000
99	G	L	0.0000
100	Q	L	-1.5621
101	G	L	0.0000
102	T	L	0.0000
103	K	L	-1.6629
104	V	L	0.0000
105	E	L	0.0000
106	I	L	-0.6669
107	K	L	-1.4881
108	R	L	-1.4162
109	T	L	-0.3261
110	V	L	0.0673
111	A	L	-0.1642
112	A	L	-0.2642
113	P	L	0.0000
114	S	L	-0.1651
115	V	L	0.0000
116	F	L	0.2562
117	I	L	0.1078
118	F	L	0.0000
119	P	L	-0.6920
120	P	L	0.0000
121	S	L	-1.3490
122	D	L	-2.6466
123	E	L	-2.1670
124	Q	L	0.0000
125	L	L	-1.8387
126	K	L	-2.5695
127	S	L	-1.5715
128	G	L	-1.3921
129	T	L	-1.1369
130	A	L	0.0000
131	S	L	0.0000
132	V	L	0.0000
133	V	L	0.0000
134	C	L	0.0000
135	L	L	0.0000
136	L	L	0.0000
137	N	L	0.0000
138	N	L	-1.4657
139	F	L	0.0000
140	Y	L	0.0000
141	P	L	-1.3415
142	R	L	-1.7569
143	E	L	-2.4735
144	A	L	-1.7307
145	K	L	-1.8308
146	V	L	-0.7034
147	Q	L	-0.2849
148	W	L	0.0000
149	K	L	-0.3895
150	V	L	0.0000
151	D	L	-1.6770
152	N	L	-1.4052
153	A	L	-0.1997
154	L	L	0.8272
155	Q	L	-0.1977
156	S	L	-0.6270
157	G	L	-1.2547
158	N	L	-1.5712
159	S	L	-1.3238
160	Q	L	-1.1800
161	E	L	-1.1063
162	S	L	-0.5320
163	V	L	-0.3584
164	T	L	-1.0316
165	E	L	-2.2261
166	Q	L	-2.2528
167	D	L	-3.0145
168	S	L	-2.3617
169	K	L	-3.0626
170	D	L	-3.2275
171	S	L	0.0000
172	T	L	0.0000
173	Y	L	0.0000
174	S	L	0.0000
175	L	L	0.0000
176	S	L	0.0000
177	S	L	0.0000
178	T	L	-0.5885
179	L	L	0.0000
180	T	L	-0.7217
181	L	L	-0.8413
182	S	L	-1.2275
183	K	L	-2.2325
184	A	L	-1.9342
185	D	L	-2.4063
186	Y	L	0.0000
187	E	L	-3.5951
188	K	L	-3.5107
189	H	L	-2.6782
190	K	L	-2.7349
191	V	L	-1.2462
192	Y	L	0.0000
193	A	L	0.0000
194	C	L	0.0000
195	E	L	-0.7704
196	V	L	0.0000
197	T	L	-1.0359
198	H	L	0.0000
199	Q	L	-1.5484
200	G	L	-0.4833
201	L	L	-0.3168
202	S	L	-0.4596
203	S	L	-0.4550
204	P	L	-0.5936
205	V	L	-0.0448
206	T	L	-0.4846
207	K	L	-0.7748
208	S	L	-0.7552
209	F	L	0.0000
210	N	L	-2.0505
211	R	L	-2.5721
212	G	L	-1.8498
213	E	L	-1.7916
214	C	L	0.0000
1	E	H	-1.9734
2	V	H	-0.6307
3	Q	H	-1.2184
4	L	H	0.0000
5	V	H	1.2535
6	E	H	0.0000
7	S	H	-0.2138
8	G	H	-0.5497
9	G	H	-0.4112
10	G	H	-0.0607
11	L	H	0.2157
12	V	H	-0.2189
13	Q	H	-1.0609
14	P	H	-1.4986
15	G	H	-1.3443
16	G	H	-1.1310
17	S	H	-1.4510
18	L	H	-1.2714
19	R	H	-2.1006
20	L	H	0.0000
21	S	H	-0.3013
22	C	H	0.0000
23	A	H	0.0040
24	A	H	0.0000
25	S	H	-0.3606
26	G	H	-0.7202
27	F	H	0.0000
28	A	H	-0.2010
29	F	H	0.0000
30	S	H	-0.8205
31	T	H	-0.3195
32	Y	H	0.0255
33	D	H	-0.2633
34	M	H	0.0000
35	S	H	0.0000
36	W	H	0.0000
37	V	H	0.0000
38	R	H	-0.6877
39	Q	H	-0.7615
40	A	H	-1.0389
41	P	H	-0.8730
42	G	H	-1.4641
43	K	H	-2.3436
44	G	H	-1.5678
45	L	H	0.0000
46	E	H	-0.8792
47	W	H	0.0000
48	V	H	0.0000
49	A	H	0.0000
50	T	H	0.0000
51	I	H	0.0000
52	S	H	-0.0772
53	S	H	0.0000
54	G	H	-0.6631
55	G	H	-0.3943
56	S	H	-0.0923
57	Y	H	1.0176
58	T	H	0.7124
59	Y	H	0.4106
60	Y	H	-0.7021
61	L	H	-1.3511
62	D	H	-2.6571
63	S	H	-1.6582
64	V	H	0.0000
65	K	H	-2.7675
66	G	H	-2.0631
67	R	H	-2.4130
68	F	H	0.0000
69	T	H	-1.0565
70	I	H	0.0000
71	S	H	-0.2379
72	R	H	-1.1094
73	D	H	-1.3768
74	S	H	-1.3356
75	S	H	-1.4066
76	K	H	-2.2410
77	N	H	-1.5990
78	T	H	-0.8927
79	L	H	0.0000
80	Y	H	-0.4064
81	L	H	0.0000
82	Q	H	-1.3790
83	M	H	0.0000
84	N	H	-2.0772
85	S	H	-1.5615
86	L	H	0.0000
87	R	H	-2.3881
88	A	H	-1.6636
89	E	H	-2.2254
90	D	H	0.0000
91	T	H	-0.4716
92	A	H	0.0000
93	V	H	0.5476
94	Y	H	0.0000
95	Y	H	0.0000
96	C	H	0.0000
97	A	H	0.0000
98	P	H	0.0000
99	T	H	0.0000
100	T	H	0.7163
101	V	H	1.3161
102	V	H	1.2043
103	P	H	0.1159
104	F	H	0.5399
105	A	H	-0.0076
106	Y	H	0.0000
107	W	H	0.0000
108	G	H	0.0000
109	Q	H	-0.9937
110	G	H	-0.3688
111	T	H	0.0000
112	L	H	1.2054
113	V	H	0.0000
114	T	H	-0.1727
115	V	H	0.0000
116	S	H	-0.8485
117	S	H	-0.6829
118	A	H	-0.4451
119	S	H	-0.5677
120	T	H	-0.8338
121	K	H	-1.3447
122	G	H	-1.4183
123	P	H	0.0000
124	S	H	-0.4833
125	V	H	0.0000
126	F	H	0.0000
127	P	H	-1.0868
128	L	H	0.0000
129	A	H	-0.9509
130	P	H	0.0000
131	S	H	-1.2810
132	S	H	-1.6033
133	K	H	-1.9857
134	S	H	-1.3762
135	T	H	-0.9431
136	S	H	-0.8029
137	G	H	-0.7775
138	G	H	-0.5963
139	T	H	-0.4541
140	A	H	-0.3644
141	A	H	0.0000
142	L	H	0.0000
143	G	H	0.0000
144	C	H	0.0000
145	L	H	0.0000
146	V	H	0.0000
147	K	H	-0.3984
148	D	H	-0.6723
149	Y	H	0.0000
150	F	H	0.0000
151	P	H	-1.0102
152	E	H	-1.2591
153	P	H	-1.3262
154	V	H	0.0000
155	T	H	-0.9611
156	V	H	-0.4682
157	S	H	-0.4041
158	W	H	0.0000
159	N	H	-0.7622
160	S	H	-0.6370
161	G	H	-0.6333
162	A	H	-0.3113
163	L	H	-0.1024
164	T	H	-0.2378
165	S	H	-0.2207
166	G	H	-0.3334
167	V	H	0.0839
168	H	H	-0.3559
169	T	H	0.0158
170	F	H	0.0000
171	P	H	-0.3901
172	A	H	-0.0881
173	V	H	0.2105
174	L	H	0.7287
175	Q	H	0.1186
176	S	H	-0.1076
177	S	H	-0.2328
178	G	H	-0.0240
179	L	H	-0.2082
180	Y	H	0.0000
181	S	H	0.0000
182	L	H	0.0000
183	S	H	0.0000
184	S	H	0.0000
185	V	H	0.0000
186	V	H	0.0000
187	T	H	-0.1117
188	V	H	0.0000
189	P	H	-0.6483
190	S	H	0.0000
191	S	H	-0.7879
192	S	H	-0.7827
193	L	H	0.0000
194	G	H	-1.2428
195	T	H	-1.1092
196	Q	H	-1.5155
197	T	H	-1.2140
198	Y	H	0.0000
199	I	H	-1.1651
200	C	H	0.0000
201	N	H	-1.2859
202	V	H	0.0000
203	N	H	-2.2775
204	H	H	0.0000
205	K	H	-2.9676
206	P	H	-1.8089
207	S	H	-1.8772
208	N	H	-2.6243
209	T	H	-2.1627
210	K	H	-2.6544
211	V	H	-1.3965
212	D	H	-1.8940
213	K	H	-1.6436
214	K	H	-2.3532
215	V	H	0.0000
216	E	H	-2.5737
217	P	H	-1.5503
218	K	H	-1.3879
219	S	H	-1.3142
220	C	H	-1.8736
221	D	H	-2.8867
222	K	H	-2.7941
223	T	H	-2.1409
224	H	H	-1.8331
225	T	H	-1.3562
226	S	H	-0.6561
227	A	H	-1.1425
228	A	H	-1.2238