Project name: test_GPF_RAD [mutate: GA10N, VA11R]

Status: done

submitted: 2018-01-12 15:47:12, status changed: 2018-01-12 16:08:31
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Chain sequence(s) A: KGEELFTGVVPILVELDGDVNGHKFSVSSSGEGEGDATYGKLTTLKFIICTTGKLPVPWPTLVTTLVQCFSRYPDHMKKQHDFFKSAMPEGYVQERTIFFKKDDGNYKKTRAEVKFEGDDTLVNRIEELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNNFKIRHNIEEDGSVQLADHYQQQNTTPIGDDGPVLLPDDNHYLSTQSALSKDPNEKRDHMVLLEEFVTAAGIT
Distance of aggregation 10 Å
Dynamic mode No
Mutated residues GA10N, VA11R
Energy difference between WT (input) and mutated protein (by FoldX) -0.525538 kcal/mol

Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

Show buried residues

Minimal score value
-3.9524
Maximal score value
0.6424
Average score
-0.999
Total score value
-224.7861

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index residue name chain Aggrescan3D score mutation
residue index residue name chain Aggrescan3D score
mutation
3 K A -2.6640
4 G A 0.0000
5 E A -3.1121
6 E A -3.0523
7 L A -1.8524
8 F A 0.0000
9 T A -1.6645
10 N A -2.2239 mutated: GA10N
11 R A -2.6557 mutated: VA11R
12 V A 0.0000
13 P A -1.7135
14 I A 0.0000
15 L A -1.0252
16 V A 0.0000
17 E A -2.0455
18 L A 0.0000
19 D A -3.4683
20 G A 0.0000
21 D A -2.8892
22 V A 0.0000
23 N A -2.1875
24 G A -1.7657
25 H A -2.1533
26 K A -3.0061
27 F A 0.0000
28 S A -1.8168
29 V A 0.0000
30 S A -1.0383
31 G A 0.0000
32 E A -2.0606
33 G A -1.6981
34 E A -2.0879
35 G A 0.0000
36 D A 0.0000
37 A A 0.0000
38 T A -0.3281
39 Y A 0.6424
40 G A 0.0000
41 K A -0.4835
42 L A 0.0000
43 T A -0.5296
44 L A 0.0000
45 K A -0.9019
46 F A 0.0000
47 I A -1.0937
48 C A 0.0000
49 T A -0.9708
50 T A -1.3453
51 G A -1.6857
52 K A -2.2866
53 L A 0.0000
54 P A -1.2574
55 V A 0.0000
56 P A 0.0000
57 W A 0.0000
58 P A 0.0000
59 T A 0.0000
60 L A 0.0000
61 V A 0.0000
62 T A -0.0037
63 T A 0.0000
64 L A 0.0000
68 V A -0.0390
69 Q A -0.1845
70 C A 0.0000
71 F A 0.0000
72 S A 0.0000
73 R A -0.9936
74 Y A -1.4404
75 P A -1.8981
76 D A -3.0188
77 H A -2.4341
78 M A 0.0000
79 K A -3.1987
80 Q A -2.4023
81 H A -1.5880
82 D A 0.0000
83 F A 0.0000
84 F A 0.0000
85 K A 0.0000
86 S A -1.3862
87 A A 0.0000
88 M A 0.0000
89 P A -1.7753
90 E A -2.3517
91 G A 0.0000
92 Y A 0.0000
93 V A 0.0000
94 Q A 0.0000
95 E A -1.8304
96 R A 0.0000
97 T A -0.3199
98 I A 0.0000
99 F A 0.3165
100 F A 0.0000
101 K A -1.7859
102 D A -2.8380
103 D A -2.4368
104 G A 0.0000
105 N A -0.7860
106 Y A 0.0000
107 K A -1.7943
108 T A 0.0000
109 R A -2.8120
110 A A 0.0000
111 E A -2.1218
112 V A 0.0000
113 K A -1.6506
114 F A -1.8101
115 E A -2.5896
116 G A -2.1723
117 D A -2.3734
118 T A -1.6489
119 L A 0.0000
120 V A 0.0000
121 N A 0.0000
122 R A -2.9806
123 I A 0.0000
124 E A -3.9384
125 L A 0.0000
126 K A -2.8465
127 G A 0.0000
128 I A -1.2344
129 D A -2.5078
130 F A 0.0000
131 K A -3.9305
132 E A -3.9524
133 D A -3.5118
134 G A -2.7638
135 N A -2.1270
136 I A 0.0000
137 L A -1.9230
138 G A -2.1807
139 H A -1.9647
140 K A -2.2563
141 L A -1.5287
142 E A -1.5707
143 Y A -0.4851
144 N A -0.5342
145 Y A -0.8623
146 N A -1.2859
147 S A -1.2699
148 H A 0.0000
149 N A -1.2204
150 V A 0.0000
151 Y A 0.5020
152 I A 0.0000
153 M A -0.7753
154 A A -1.5282
155 D A 0.0000
156 K A -3.0544
157 Q A -2.9599
158 K A -3.0016
159 N A -2.8408
160 G A 0.0000
161 I A 0.0000
162 K A -1.0047
163 V A 0.0000
164 N A -0.2812
165 F A 0.0000
166 K A -1.8525
167 I A 0.0000
168 R A -1.6912
169 H A 0.0000
170 N A -1.4810
171 I A 0.0000
172 E A -3.2064
173 D A -3.0091
174 G A -1.9075
175 S A -1.0378
176 V A -0.3751
177 Q A 0.0000
178 L A -1.0128
179 A A 0.0000
180 D A 0.0000
181 H A 0.0000
182 Y A 0.6038
183 Q A 0.0000
184 Q A -0.8512
185 N A 0.0000
186 T A -1.0728
187 P A -0.9599
188 I A -0.4401
189 G A -1.3668
190 D A -2.2584
191 G A -1.5947
192 P A -0.7755
193 V A -0.3124
194 L A -0.2078
195 L A -0.5562
196 P A 0.0000
197 D A -2.3144
198 N A -1.7724
199 H A 0.0000
200 Y A -0.0172
201 L A 0.0000
202 S A -0.5780
203 T A -0.8400
204 Q A -1.3092
205 S A -0.5378
206 A A -0.0056
207 L A -0.0080
208 S A -0.6973
209 K A -1.9664
210 D A -2.1475
211 P A -1.8494
212 N A -2.4849
213 E A -2.7276
214 K A -3.3089
215 R A -3.3832
216 D A -2.2754
217 H A 0.0000
218 M A 0.0000
219 V A -0.2791
220 L A 0.0000
221 L A 0.4245
222 E A -0.0914
223 F A 0.2115
224 V A 0.0000
225 T A -0.3306
226 A A 0.0000
227 A A -0.3687
228 G A -0.4859
229 I A -0.4847
230 T A -0.1849

 

Laboratory of Theory of Biopolymers 2015