Project name: 974d67e6640fd9a [mutate: QA214H]

Status: done

submitted: 2018-07-12 09:14:33, status changed: 2018-07-13 17:05:55
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Chain sequence(s) A: SKRQLFLLPGGLERHLKIKTCTVALDVVEEICAEMALTRPEAFNEYVIFVVTNRGQHVCPLSRRAYILDVASEMEQVDGGYMLWFRRVLWDQPLKFENELYVTMHYNQVLPDYLKGLFSSVPASRPSEQLLQQVSKLASLQHRAKDHFYLPSVREVQEYIPAQLYRTTAGSTWLNLVSQHRQQTQALSPHQARAQFLGLLSALPMFGSSFFFIQSCSNIAVPAPCILAINHNGLNFLSTETHELMVKFPLKEIQSTRTQRPTANSSYPYVEIALGDVAAQRTLQLQLEQGLELCRVVAVHVENLLSAHEKRLTLPPSEITLL
Distance of aggregation 10 Å
Dynamic mode No
Mutated residues QA214H
Energy difference between WT (input) and mutated protein (by FoldX) 0.132985 kcal/mol

Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

Show buried residues

Minimal score value
-3.2002
Maximal score value
1.949
Average score
-0.5876
Total score value
-189.2023

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index residue name chain Aggrescan3D score mutation
residue index residue name chain Aggrescan3D score
mutation
1 S A -1.7387
2 K A -2.8145
3 R A -3.0882
4 Q A -1.5680
5 L A -0.5878
6 F A 0.0000
7 L A -0.1238
8 L A 0.0000
9 P A -0.4119
10 G A -0.6076
11 G A -0.4809
12 L A 0.0184
13 E A -1.0714
14 R A -1.1769
15 H A -1.6959
16 L A 0.0000
17 K A -2.7227
18 I A 0.0000
19 K A -1.7163
20 T A -0.4474
21 C A 0.3379
22 T A 0.0000
23 V A -0.1960
24 A A 0.0000
25 L A -0.9109
26 D A -1.7208
27 V A 0.0000
28 V A 0.0000
29 E A -2.2650
30 E A -1.6210
31 I A 0.0000
32 C A 0.0000
33 A A -0.8311
34 E A -1.0890
35 M A 0.0000
36 A A -0.5763
37 L A 0.0000
38 T A -0.6764
39 R A 0.0000
40 P A -1.2554
41 E A -2.0955
42 A A 0.0000
43 F A 0.0000
44 N A -0.8697
45 E A 0.0000
46 Y A 0.0000
47 V A 0.0000
48 I A 0.0000
49 F A 0.9030
50 V A 0.0000
51 V A -0.0191
52 T A -1.2510
53 N A -2.1605
54 R A -2.7143
55 G A -1.2293
56 Q A -1.6523
57 H A -0.3920
58 V A 0.6564
59 C A 0.3919
60 P A -0.0012
61 L A 0.0000
62 S A -0.6913
63 R A -1.1897
64 R A -1.8095
65 A A -0.5762
66 Y A 0.1059
67 I A 0.0000
68 L A -0.4981
69 D A -1.7115
70 V A -1.1644
71 A A 0.0000
72 S A -1.9140
73 E A -2.6964
74 M A -1.4216
75 E A -2.1144
76 Q A -1.9360
77 V A -0.4211
78 D A -1.8460
79 G A -1.0923
80 G A -0.3061
81 Y A 0.3690
82 M A 1.0023
83 L A 0.0000
84 W A 0.4960
85 F A 0.0000
86 R A -0.2894
87 R A 0.0000
88 V A 0.0000
89 L A 0.0000
90 W A 0.0000
91 D A -1.1877
92 Q A -0.8277
93 P A 0.0000
94 L A -0.1704
95 K A -1.4985
96 F A -0.7230
97 E A -1.1889
98 N A -1.9567
99 E A -1.3538
100 L A 0.0000
101 Y A -0.6870
102 V A 0.0000
103 T A 0.0000
104 M A 0.0000
105 H A 0.0000
106 Y A 0.0000
107 N A -0.5762
108 Q A -0.4095
109 V A -0.0723
110 L A 0.0000
111 P A -0.9084
112 D A -0.7898
113 Y A 0.0000
114 L A -1.3021
115 K A -1.9141
116 G A 0.0000
117 L A -0.3978
118 F A 0.1021
119 S A 0.1349
120 S A 0.8456
121 V A 1.6530
122 P A 0.6029
123 A A 0.5161
124 S A 0.5493
125 R A 0.0000
126 P A 0.0000
127 S A -0.3950
128 E A 0.0000
129 Q A 0.0000
130 L A 0.0000
131 L A 0.0000
132 Q A 0.0000
133 Q A 0.0000
134 V A 0.0000
135 S A 0.0000
136 K A -1.7637
137 L A 0.0000
138 A A -1.4379
139 S A -1.4256
140 L A -1.4809
141 Q A -2.5745
142 H A -2.8035
143 R A -3.0693
144 A A 0.0000
145 K A -2.4996
146 D A -1.7227
147 H A -1.2541
148 F A -0.0105
149 Y A 0.4044
150 L A 1.3810
151 P A 0.5880
152 S A 0.5004
153 V A 0.2791
154 R A -1.9509
155 E A -1.7667
156 V A 0.0000
157 Q A -2.3349
158 E A -2.9386
159 Y A 0.0000
160 I A 0.0000
161 P A 0.0000
162 A A -1.4221
163 Q A -1.5305
164 L A -0.6098
165 Y A -0.8277
166 R A -1.9125
167 T A -1.0031
168 T A -0.5668
169 A A -0.3497
170 G A -0.4381
171 S A -0.1127
172 T A -0.4838
173 W A 0.0000
174 L A 0.0000
175 N A -1.3792
176 L A -0.6562
177 V A 0.0000
178 S A -1.3841
179 Q A -2.0264
180 H A -1.7017
181 R A 0.0000
182 Q A -2.1558
183 Q A -2.2415
184 T A -1.5620
185 Q A -1.6952
186 A A -0.7356
187 L A -0.6823
188 S A -0.7988
189 P A -1.2458
190 H A -1.7139
191 Q A -1.7121
192 A A 0.0000
193 R A -1.3573
194 A A -1.5211
195 Q A -1.4450
196 F A 0.0000
197 L A 0.0000
198 G A -0.6510
199 L A -0.0605
200 L A 0.0000
201 S A -0.1486
202 A A 0.1643
203 L A 0.3111
204 P A -0.0368
205 M A 0.0000
206 F A 0.0949
207 G A -0.3106
208 S A 0.0000
209 S A 0.0000
210 F A 0.4331
211 F A 0.0000
212 F A 1.3327
213 I A 0.0000
214 H A -0.3017 mutated: QA214H
215 S A 0.0000
216 C A 0.6647
217 S A 0.1946
218 N A 0.4826
219 I A 1.9490
220 A A 0.9897
221 V A 0.8664
222 P A 0.0640
223 A A 0.1529
224 P A -0.1922
225 C A 0.0000
226 I A 0.0000
227 L A 0.0000
228 A A 0.0000
229 I A 0.0000
230 N A 0.0000
231 H A -1.0328
232 N A -1.7239
233 G A 0.0000
234 L A 0.0000
235 N A 0.0000
236 F A 0.0000
237 L A 0.0000
238 S A 0.0000
239 T A -0.7958
240 E A -2.1573
241 T A -1.2997
242 H A 0.0000
243 E A -0.8456
244 L A 0.6729
245 M A 0.7187
246 V A 0.4683
247 K A -0.5617
248 F A 0.0000
249 P A -1.4235
250 L A 0.0000
251 K A -2.1829
252 E A -2.1763
253 I A -1.6521
254 Q A -1.9727
255 S A -1.2421
256 T A -1.2120
257 R A -1.8099
258 T A -1.0782
259 Q A -0.9812
260 R A -0.4817
261 P A -0.6070
262 T A -0.5132
263 A A -0.6161
264 N A -1.2405
265 S A -0.7223
266 S A -0.4439
267 Y A -0.1351
268 P A -0.2310
269 Y A -0.4450
270 V A 0.0000
271 E A -1.0015
272 I A 0.0000
273 A A -1.5645
274 L A 0.0000
275 G A -2.0879
276 D A -2.2438
277 V A -0.9645
278 A A -0.7250
279 A A -0.8605
280 Q A -2.0284
281 R A -1.9990
282 T A -1.0878
283 L A 0.0000
284 Q A 0.0000
285 L A 0.0000
286 Q A -0.1746
287 L A 0.1120
288 E A -0.3487
289 Q A -0.2591
290 G A 0.0000
291 L A 0.1803
292 E A 0.0000
293 L A 0.0000
294 C A -0.3121
295 R A -0.1305
296 V A 0.0000
297 V A 0.0000
298 A A -0.1327
299 V A 0.0004
300 H A 0.0000
301 V A 0.0510
302 E A -0.3977
303 N A -0.6915
304 L A -0.4572
305 L A -0.4291
306 S A -1.3886
307 A A -1.4979
308 H A -2.1467
309 E A -3.1155
310 K A -3.2002
311 R A -2.6316
312 L A -0.1546
313 T A -0.0468
314 L A -0.1326
315 P A -0.3107
316 P A -0.3554
317 S A -0.3973
318 E A -0.5077
319 I A 0.5399
320 T A -0.1613
321 L A -0.2714
322 L A 0.1358

 

Laboratory of Theory of Biopolymers 2015