| Chain sequence(s) |
A: MDTVCIAVVGAGVIGLSTAACISQLVPGCTVTVISDRFTPDTTSNVAAGMLIPHTYADTPVPTQKRWFRETFEHLSEIAKSAEAADAGVHLVSGWQIFHSVPAEEVPFWADVVLGFRKMTEAELKRFPQYVFGQAFTTLKCETSAYLPWLERRIKGSGGLLLTWRIEDLWELQPSFDIVVNCSGLGSRRLVGDPMISPVRGQVLQARAPWVKHFIRDGGGLTYVYPGMSYVTLGGTRQKGDWNRSPDAELSREIFSRCCTLEPSLHRAYDIKEKVGLRPSRPGVRLQKEILVRGQQTLPVVHNYGHGSGGISVHWGSALEATRLVMECIHTLRTPASLSKL |
| Distance of aggregation | 10 Å |
| Dynamic mode | No |
| Mutated residues | VA101T, LA161T, IA290L |
| Energy difference between WT (input) and mutated protein (by FoldX) | 2.61825 kcal/mol
CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 1 | M | A | 0.1118 | |
| 2 | D | A | -1.3415 | |
| 3 | T | A | -0.7954 | |
| 4 | V | A | 0.0000 | |
| 5 | C | A | -0.4189 | |
| 6 | I | A | 0.0000 | |
| 7 | A | A | 0.0000 | |
| 8 | V | A | 0.0000 | |
| 9 | V | A | 0.0000 | |
| 10 | G | A | -0.8102 | |
| 11 | A | A | 0.0000 | |
| 12 | G | A | -0.4056 | |
| 13 | V | A | 0.0000 | |
| 14 | I | A | 0.1618 | |
| 15 | G | A | 0.0000 | |
| 16 | L | A | 0.0000 | |
| 17 | S | A | 0.0000 | |
| 18 | T | A | 0.0000 | |
| 19 | A | A | 0.0000 | |
| 20 | A | A | -0.7973 | |
| 21 | C | A | 0.0000 | |
| 22 | I | A | 0.0000 | |
| 23 | S | A | -0.7090 | |
| 24 | Q | A | -1.2251 | |
| 25 | L | A | 0.0000 | |
| 26 | V | A | 0.0000 | |
| 27 | P | A | -0.6892 | |
| 28 | G | A | -0.9130 | |
| 29 | C | A | -0.6890 | |
| 30 | T | A | -0.2739 | |
| 31 | V | A | 0.0000 | |
| 32 | T | A | 0.7598 | |
| 33 | V | A | 0.0000 | |
| 34 | I | A | 0.0000 | |
| 35 | S | A | 0.0000 | |
| 36 | D | A | -3.2084 | |
| 37 | R | A | -2.9346 | |
| 38 | F | A | -1.1798 | |
| 39 | T | A | 0.0000 | |
| 40 | P | A | -0.8087 | |
| 41 | D | A | -1.3956 | |
| 42 | T | A | -1.0275 | |
| 43 | T | A | -0.4869 | |
| 44 | S | A | 0.0000 | |
| 45 | N | A | 0.0000 | |
| 46 | V | A | -0.1675 | |
| 47 | A | A | -0.0622 | |
| 48 | A | A | -0.0260 | |
| 49 | G | A | 0.0000 | |
| 50 | M | A | 0.0000 | |
| 51 | L | A | 0.0000 | |
| 52 | I | A | 0.1142 | |
| 53 | P | A | -0.1996 | |
| 54 | H | A | -0.4813 | |
| 55 | T | A | -0.3114 | |
| 56 | Y | A | 0.0155 | |
| 57 | A | A | -0.4543 | |
| 58 | D | A | -0.7818 | |
| 59 | T | A | -0.3496 | |
| 60 | P | A | -0.3150 | |
| 61 | V | A | -0.3031 | |
| 62 | P | A | -0.7121 | |
| 63 | T | A | -0.6900 | |
| 64 | Q | A | -0.8369 | |
| 65 | K | A | -1.2118 | |
| 66 | R | A | -1.4813 | |
| 67 | W | A | 0.0000 | |
| 68 | F | A | 0.0000 | |
| 69 | R | A | -1.9737 | |
| 70 | E | A | -1.6638 | |
| 71 | T | A | 0.0000 | |
| 72 | F | A | 0.0000 | |
| 73 | E | A | -2.7935 | |
| 74 | H | A | -2.1251 | |
| 75 | L | A | 0.0000 | |
| 76 | S | A | -1.9922 | |
| 77 | E | A | -2.8333 | |
| 78 | I | A | -1.9979 | |
| 79 | A | A | -1.8438 | |
| 80 | K | A | -2.3872 | |
| 81 | S | A | -1.6995 | |
| 82 | A | A | -1.4436 | |
| 83 | E | A | -2.5288 | |
| 84 | A | A | -2.0013 | |
| 85 | A | A | -1.6730 | |
| 86 | D | A | -2.4049 | |
| 87 | A | A | 0.0000 | |
| 88 | G | A | 0.0000 | |
| 89 | V | A | 0.0000 | |
| 90 | H | A | -0.7492 | |
| 91 | L | A | 0.4718 | |
| 92 | V | A | -0.0666 | |
| 93 | S | A | -0.0968 | |
| 94 | G | A | 0.0000 | |
| 95 | W | A | -0.2623 | |
| 96 | Q | A | 0.0000 | |
| 97 | I | A | 0.0000 | |
| 98 | F | A | 0.3124 | |
| 99 | H | A | -0.3190 | |
| 100 | S | A | 0.1396 | |
| 101 | T | A | -0.1624 | mutated: VA101T |
| 102 | P | A | -0.6274 | |
| 103 | A | A | -0.7043 | |
| 104 | E | A | -1.5280 | |
| 105 | E | A | 0.0000 | |
| 106 | V | A | -0.3875 | |
| 107 | P | A | -0.4309 | |
| 108 | F | A | -0.2794 | |
| 109 | W | A | 0.0000 | |
| 110 | A | A | -0.0243 | |
| 111 | D | A | -1.1456 | |
| 112 | V | A | 0.0000 | |
| 113 | V | A | 0.0000 | |
| 114 | L | A | 1.0716 | |
| 115 | G | A | 0.4037 | |
| 116 | F | A | 0.8763 | |
| 117 | R | A | -0.9521 | |
| 118 | K | A | -1.8905 | |
| 119 | M | A | 0.0000 | |
| 120 | T | A | -1.6678 | |
| 121 | E | A | -2.3927 | |
| 122 | A | A | -2.0170 | |
| 123 | E | A | -2.1415 | |
| 124 | L | A | -1.8200 | |
| 125 | K | A | -3.0789 | |
| 126 | R | A | -3.1720 | |
| 127 | F | A | 0.0000 | |
| 128 | P | A | -1.5102 | |
| 129 | Q | A | -1.4145 | |
| 130 | Y | A | -0.2371 | |
| 131 | V | A | 0.9831 | |
| 132 | F | A | 0.5839 | |
| 133 | G | A | 0.0000 | |
| 134 | Q | A | 0.0000 | |
| 135 | A | A | 0.0000 | |
| 136 | F | A | 0.0000 | |
| 137 | T | A | 0.7628 | |
| 138 | T | A | 0.0000 | |
| 139 | L | A | 0.0000 | |
| 140 | K | A | 0.0000 | |
| 141 | C | A | 0.0000 | |
| 142 | E | A | -0.5248 | |
| 143 | T | A | 0.0000 | |
| 144 | S | A | -0.3552 | |
| 145 | A | A | -0.5037 | |
| 146 | Y | A | 0.0000 | |
| 147 | L | A | 0.0000 | |
| 148 | P | A | -1.0498 | |
| 149 | W | A | -1.7234 | |
| 150 | L | A | 0.0000 | |
| 151 | E | A | -1.8922 | |
| 152 | R | A | -3.3997 | |
| 153 | R | A | -3.4457 | |
| 154 | I | A | 0.0000 | |
| 155 | K | A | -2.7114 | |
| 156 | G | A | -2.3606 | |
| 157 | S | A | -1.7853 | |
| 158 | G | A | -0.9762 | |
| 159 | G | A | -0.5975 | |
| 160 | L | A | 1.2072 | |
| 161 | T | A | 0.9348 | mutated: LA161T |
| 162 | L | A | 0.7354 | |
| 163 | T | A | -0.7411 | |
| 164 | W | A | -1.3231 | |
| 165 | R | A | -2.9058 | |
| 166 | I | A | 0.0000 | |
| 167 | E | A | -2.1744 | |
| 168 | D | A | -1.8684 | |
| 169 | L | A | 0.0000 | |
| 170 | W | A | -0.2742 | |
| 171 | E | A | -1.6663 | |
| 172 | L | A | 0.0000 | |
| 173 | Q | A | -0.7666 | |
| 174 | P | A | -0.6337 | |
| 175 | S | A | -0.5841 | |
| 176 | F | A | -0.1424 | |
| 177 | D | A | -0.4515 | |
| 178 | I | A | 0.0000 | |
| 179 | V | A | 0.0000 | |
| 180 | V | A | 0.0000 | |
| 181 | N | A | 0.0000 | |
| 182 | C | A | 0.0000 | |
| 183 | S | A | -0.6901 | |
| 184 | G | A | -0.3147 | |
| 185 | L | A | -0.4408 | |
| 186 | G | A | -1.0122 | |
| 187 | S | A | 0.0000 | |
| 188 | R | A | -1.9641 | |
| 189 | R | A | -2.5985 | |
| 190 | L | A | 0.0000 | |
| 191 | V | A | -0.9224 | |
| 192 | G | A | -1.1147 | |
| 193 | D | A | 0.0000 | |
| 194 | P | A | -0.3197 | |
| 195 | M | A | 0.0186 | |
| 196 | I | A | 0.0000 | |
| 197 | S | A | -0.6465 | |
| 198 | P | A | 0.0000 | |
| 199 | V | A | -1.0693 | |
| 200 | R | A | 0.0000 | |
| 201 | G | A | 0.0000 | |
| 202 | Q | A | 0.0000 | |
| 203 | V | A | 0.0000 | |
| 204 | L | A | 0.0000 | |
| 205 | Q | A | -0.9531 | |
| 206 | A | A | 0.0000 | |
| 207 | R | A | -0.7547 | |
| 208 | A | A | 0.0000 | |
| 209 | P | A | -0.2784 | |
| 210 | W | A | -0.0185 | |
| 211 | V | A | -0.6010 | |
| 212 | K | A | -1.6927 | |
| 213 | H | A | -1.0647 | |
| 214 | F | A | 0.0000 | |
| 215 | I | A | 0.0000 | |
| 216 | R | A | 0.0000 | |
| 217 | D | A | 0.0000 | |
| 218 | G | A | -0.3648 | |
| 219 | G | A | -0.5641 | |
| 220 | G | A | -0.6081 | |
| 221 | L | A | -0.2065 | |
| 222 | T | A | 0.0000 | |
| 223 | Y | A | 0.0000 | |
| 224 | V | A | 0.0000 | |
| 225 | Y | A | 0.0000 | |
| 226 | P | A | 0.0000 | |
| 227 | G | A | -0.2573 | |
| 228 | M | A | 0.1428 | |
| 229 | S | A | 0.0553 | |
| 230 | Y | A | 0.1100 | |
| 231 | V | A | 0.0000 | |
| 232 | T | A | 0.0000 | |
| 233 | L | A | 0.0000 | |
| 234 | G | A | 0.0000 | |
| 235 | G | A | 0.0000 | |
| 236 | T | A | 0.0000 | |
| 237 | R | A | -1.3275 | |
| 238 | Q | A | -1.7291 | |
| 239 | K | A | -2.1936 | |
| 240 | G | A | -1.3956 | |
| 241 | D | A | -1.2054 | |
| 242 | W | A | -0.2996 | |
| 243 | N | A | -1.5468 | |
| 244 | R | A | -2.2185 | |
| 245 | S | A | -1.4721 | |
| 246 | P | A | -1.4202 | |
| 247 | D | A | -1.7436 | |
| 248 | A | A | -1.6700 | |
| 249 | E | A | -2.5954 | |
| 250 | L | A | -1.5474 | |
| 251 | S | A | -1.7599 | |
| 252 | R | A | -2.6673 | |
| 253 | E | A | -1.7301 | |
| 254 | I | A | 0.0000 | |
| 255 | F | A | -0.9062 | |
| 256 | S | A | -1.0566 | |
| 257 | R | A | -1.0058 | |
| 258 | C | A | 0.0000 | |
| 259 | C | A | 0.0000 | |
| 260 | T | A | -0.7429 | |
| 261 | L | A | 0.0000 | |
| 262 | E | A | -0.8616 | |
| 263 | P | A | -1.1077 | |
| 264 | S | A | -1.0688 | |
| 265 | L | A | 0.0000 | |
| 266 | H | A | -1.7831 | |
| 267 | R | A | -2.3010 | |
| 268 | A | A | -1.0835 | |
| 269 | Y | A | -0.2424 | |
| 270 | D | A | -1.2870 | |
| 271 | I | A | -0.9132 | |
| 272 | K | A | -2.1807 | |
| 273 | E | A | -1.9042 | |
| 274 | K | A | -1.3811 | |
| 275 | V | A | 0.0000 | |
| 276 | G | A | -0.6442 | |
| 277 | L | A | 0.0000 | |
| 278 | R | A | -0.7057 | |
| 279 | P | A | 0.0000 | |
| 280 | S | A | -0.5882 | |
| 281 | R | A | -0.3515 | |
| 282 | P | A | -0.3337 | |
| 283 | G | A | -0.1860 | |
| 284 | V | A | -0.0662 | |
| 285 | R | A | -0.3276 | |
| 286 | L | A | -0.3734 | |
| 287 | Q | A | -1.0214 | |
| 288 | K | A | -1.0186 | |
| 289 | E | A | -0.0542 | |
| 290 | L | A | 1.4559 | mutated: IA290L |
| 291 | L | A | 1.3633 | |
| 292 | V | A | 1.3100 | |
| 293 | R | A | -0.3456 | |
| 294 | G | A | -1.1221 | |
| 295 | Q | A | -1.5955 | |
| 296 | Q | A | -1.2671 | |
| 297 | T | A | 0.0929 | |
| 298 | L | A | 0.0000 | |
| 299 | P | A | 0.0000 | |
| 300 | V | A | 0.0000 | |
| 301 | V | A | 0.0000 | |
| 302 | H | A | 0.0000 | |
| 303 | N | A | 0.0000 | |
| 304 | Y | A | 0.0000 | |
| 305 | G | A | 0.0000 | |
| 306 | H | A | 0.0000 | |
| 307 | G | A | -0.4346 | |
| 308 | S | A | -0.4697 | |
| 309 | G | A | -0.2984 | |
| 310 | G | A | -0.1381 | |
| 311 | I | A | 0.0000 | |
| 312 | S | A | 0.0000 | |
| 313 | V | A | 0.0000 | |
| 314 | H | A | 0.0000 | |
| 315 | W | A | -0.1916 | |
| 316 | G | A | 0.0000 | |
| 317 | S | A | 0.0000 | |
| 318 | A | A | 0.0000 | |
| 319 | L | A | -0.0205 | |
| 320 | E | A | 0.0000 | |
| 321 | A | A | 0.0000 | |
| 322 | T | A | 0.0000 | |
| 323 | R | A | -0.9800 | |
| 324 | L | A | 0.0000 | |
| 325 | V | A | 0.0000 | |
| 326 | M | A | -0.4311 | |
| 327 | E | A | -0.9288 | |
| 328 | C | A | 0.0000 | |
| 329 | I | A | 0.0000 | |
| 330 | H | A | -0.8761 | |
| 331 | T | A | -0.5328 | |
| 332 | L | A | -0.3893 | |
| 333 | R | A | -1.7103 | |
| 334 | T | A | -0.8911 | |
| 335 | P | A | -0.8724 | |
| 336 | A | A | -0.5756 | |
| 337 | S | A | -0.5299 | |
| 338 | L | A | -0.7522 | |
| 339 | S | A | -0.9711 | |
| 340 | K | A | -1.7210 | |
| 341 | L | A | -0.8864 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine