Project name: LC-S176W [mutate: SL176W]

Chain sequence(s)	H: EVQLVESGGGLVQPGGSLRLSCAASGFAFSTYDMSWVRQAPGKGLEWVATISSGGSYTYYLDSVKGRFTISRDSSKNTLYLQMNSLRAEDTAVYYCAPTTVVPFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTSAA; L: DIQMTQSPSSLSASVGDRVTITCKASQNVRTVVAWYQQKPGKAPKTLIYLASNRHTGVPSRFSGSGSGTDFTLTISSLQPEDFATYFCLQHWSYPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SL176W
Energy difference between WT (input) and mutated protein (by FoldX)	1.19685 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.7192
• Maximal score value: 1.3012
• Average score: -0.7209
• Total score value: -318.6514

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	D	L	-2.4047
2	I	L	0.0000
3	Q	L	-2.0435
4	M	L	0.0000
5	T	L	-1.1056
6	Q	L	0.0000
7	S	L	-0.6023
8	P	L	-0.5589
9	S	L	-0.8399
10	S	L	-0.7074
11	L	L	-0.4816
12	S	L	-0.8713
13	A	L	0.0000
14	S	L	-0.5234
15	V	L	0.4442
16	G	L	-0.5836
17	D	L	-1.5498
18	R	L	-2.2395
19	V	L	0.0000
20	T	L	-0.5906
21	I	L	0.0000
22	T	L	-0.7518
23	C	L	0.0000
24	K	L	-2.1071
25	A	L	-1.9775
26	S	L	-2.0858
27	Q	L	-2.6921
28	N	L	-2.7267
29	V	L	0.0000
30	R	L	-2.5176
31	T	L	-1.2505
32	V	L	0.0000
33	V	L	0.0000
34	A	L	0.0000
35	W	L	0.0000
36	Y	L	0.0000
37	Q	L	0.0000
38	Q	L	-1.3609
39	K	L	-1.9152
40	P	L	-1.8054
41	G	L	-1.5559
42	K	L	-2.5372
43	A	L	-1.7566
44	P	L	0.0000
45	K	L	-1.7358
46	T	L	0.0000
47	L	L	0.0000
48	I	L	0.0000
49	Y	L	-0.0023
50	L	L	0.0482
51	A	L	0.0000
52	S	L	-0.9381
53	N	L	-1.3624
54	R	L	-2.2482
55	H	L	0.0000
56	T	L	-1.1816
57	G	L	-1.3246
58	V	L	0.0000
59	P	L	-0.7481
60	S	L	-0.8810
61	R	L	-1.0623
62	F	L	0.0000
63	S	L	-0.9274
64	G	L	0.0000
65	S	L	-0.6697
66	G	L	-1.2644
67	S	L	-1.7114
68	G	L	-2.2651
69	T	L	-2.3564
70	D	L	-2.6667
71	F	L	0.0000
72	T	L	-0.7465
73	L	L	0.0000
74	T	L	-0.6244
75	I	L	0.0000
76	S	L	-1.2979
77	S	L	-1.0831
78	L	L	0.0000
79	Q	L	-0.9910
80	P	L	-0.9771
81	E	L	-1.9898
82	D	L	0.0000
83	F	L	-0.6380
84	A	L	0.0000
85	T	L	-0.9062
86	Y	L	0.0000
87	F	L	0.0000
88	C	L	0.0000
89	L	L	0.0000
90	Q	L	0.0000
91	H	L	0.4095
92	W	L	0.1087
93	S	L	0.0428
94	Y	L	0.2526
95	P	L	-0.3376
96	L	L	0.0000
97	T	L	-0.7084
98	F	L	0.0000
99	G	L	0.0000
100	Q	L	-1.5518
101	G	L	0.0000
102	T	L	0.0000
103	K	L	-1.5679
104	V	L	0.0000
105	E	L	0.0000
106	I	L	-0.5692
107	K	L	-1.4288
108	R	L	-1.3012
109	T	L	-0.2490
110	V	L	0.1517
111	A	L	-0.1236
112	A	L	-0.2554
113	P	L	0.0000
114	S	L	-0.1699
115	V	L	0.0000
116	F	L	0.2538
117	I	L	0.1051
118	F	L	0.0000
119	P	L	-0.6983
120	P	L	0.0000
121	S	L	-1.3436
122	D	L	-2.6369
123	E	L	-2.1529
124	Q	L	0.0000
125	L	L	-1.8275
126	K	L	-2.5607
127	S	L	-1.5637
128	G	L	-1.3772
129	T	L	-1.1230
130	A	L	0.0000
131	S	L	0.0000
132	V	L	0.0000
133	V	L	0.0000
134	C	L	0.0000
135	L	L	0.0000
136	L	L	0.0000
137	N	L	0.0000
138	N	L	-1.3969
139	F	L	0.0000
140	Y	L	0.0000
141	P	L	-1.3044
142	R	L	-1.7348
143	E	L	-2.4647
144	A	L	-1.7251
145	K	L	-1.8289
146	V	L	-0.6951
147	Q	L	-0.2859
148	W	L	0.0000
149	K	L	-0.4164
150	V	L	0.0000
151	D	L	-1.7699
152	N	L	-1.4676
153	A	L	-0.2174
154	L	L	0.8194
155	Q	L	-0.2100
156	S	L	-0.6290
157	G	L	-1.2430
158	N	L	-1.5468
159	S	L	-1.2815
160	Q	L	-1.1542
161	E	L	-1.0728
162	S	L	-0.5329
163	V	L	-0.3180
164	T	L	-0.9182
165	E	L	-2.0547
166	Q	L	-1.9414
167	D	L	-2.3940
168	S	L	-2.0594
169	K	L	-2.8633
170	D	L	-3.0199
171	S	L	0.0000
172	T	L	0.0000
173	Y	L	0.0000
174	S	L	0.0000
175	L	L	0.0000
176	W	L	0.0000	mutated: SL176W
177	S	L	0.0000
178	T	L	0.0000
179	L	L	0.0000
180	T	L	-0.7025
181	L	L	-0.8294
182	S	L	-1.2121
183	K	L	-2.2006
184	A	L	-1.9184
185	D	L	-2.3958
186	Y	L	0.0000
187	E	L	-3.7192
188	K	L	-3.6166
189	H	L	-2.8975
190	K	L	-3.1582
191	V	L	-1.4853
192	Y	L	0.0000
193	A	L	0.0000
194	C	L	0.0000
195	E	L	-0.7694
196	V	L	0.0000
197	T	L	-1.0338
198	H	L	0.0000
199	Q	L	-1.5382
200	G	L	-0.4686
201	L	L	-0.3111
202	S	L	-0.4596
203	S	L	-0.4536
204	P	L	-0.5906
205	V	L	-0.0389
206	T	L	-0.4848
207	K	L	-0.7816
208	S	L	-0.7419
209	F	L	0.0000
210	N	L	-2.0042
211	R	L	-2.8903
212	G	L	-1.9708
213	E	L	-1.8199
214	C	L	0.0000
1	E	H	-1.9562
2	V	H	-0.6307
3	Q	H	-1.2102
4	L	H	0.0000
5	V	H	1.2444
6	E	H	0.0000
7	S	H	-0.2156
8	G	H	-0.5477
9	G	H	-0.4059
10	G	H	-0.0527
11	L	H	0.2292
12	V	H	-0.2108
13	Q	H	-1.0682
14	P	H	-1.4997
15	G	H	-1.3444
16	G	H	-1.1316
17	S	H	-1.4508
18	L	H	-1.2707
19	R	H	-2.1016
20	L	H	0.0000
21	S	H	-0.3025
22	C	H	0.0000
23	A	H	0.0109
24	A	H	0.0000
25	S	H	-0.3568
26	G	H	-0.7201
27	F	H	0.0000
28	A	H	-0.1996
29	F	H	0.0000
30	S	H	-0.8209
31	T	H	-0.3237
32	Y	H	0.0158
33	D	H	-0.2797
34	M	H	0.0000
35	S	H	0.0000
36	W	H	0.0000
37	V	H	0.0000
38	R	H	-0.6919
39	Q	H	-0.7618
40	A	H	-1.0392
41	P	H	-0.8726
42	G	H	-1.4639
43	K	H	-2.3443
44	G	H	-1.5691
45	L	H	0.0000
46	E	H	-0.8832
47	W	H	0.0000
48	V	H	0.0000
49	A	H	0.0000
50	T	H	0.0000
51	I	H	0.0000
52	S	H	-0.0864
53	S	H	0.0000
54	G	H	-0.6669
55	G	H	-0.3927
56	S	H	-0.0942
57	Y	H	1.0160
58	T	H	0.7126
59	Y	H	0.4138
60	Y	H	-0.6952
61	L	H	-1.3435
62	D	H	-2.6513
63	S	H	-1.6454
64	V	H	0.0000
65	K	H	-2.7616
66	G	H	-2.0559
67	R	H	-2.3984
68	F	H	0.0000
69	T	H	-1.0546
70	I	H	0.0000
71	S	H	-0.2471
72	R	H	-1.1231
73	D	H	-1.3752
74	S	H	-1.3306
75	S	H	-1.4015
76	K	H	-2.2322
77	N	H	-1.5807
78	T	H	-0.8878
79	L	H	0.0000
80	Y	H	-0.4157
81	L	H	0.0000
82	Q	H	-1.3796
83	M	H	0.0000
84	N	H	-2.0745
85	S	H	-1.5588
86	L	H	0.0000
87	R	H	-2.3766
88	A	H	-1.6599
89	E	H	-2.2215
90	D	H	0.0000
91	T	H	-0.4670
92	A	H	0.0000
93	V	H	0.5461
94	Y	H	0.0000
95	Y	H	0.0000
96	C	H	0.0000
97	A	H	0.0000
98	P	H	0.0000
99	T	H	0.0000
100	T	H	0.7004
101	V	H	1.3012
102	V	H	1.1914
103	P	H	0.1036
104	F	H	0.5368
105	A	H	0.0150
106	Y	H	0.0000
107	W	H	0.0000
108	G	H	0.0000
109	Q	H	-1.0364
110	G	H	-0.3854
111	T	H	0.0000
112	L	H	1.2049
113	V	H	0.0000
114	T	H	-0.1664
115	V	H	0.0000
116	S	H	-0.8481
117	S	H	-0.6849
118	A	H	-0.4486
119	S	H	-0.5730
120	T	H	-0.8421
121	K	H	-1.3653
122	G	H	-1.4275
123	P	H	0.0000
124	S	H	-0.4884
125	V	H	0.0000
126	F	H	0.0000
127	P	H	-1.0845
128	L	H	0.0000
129	A	H	-0.9486
130	P	H	0.0000
131	S	H	-1.2782
132	S	H	-1.5984
133	K	H	-1.9823
134	S	H	-1.3722
135	T	H	-0.9396
136	S	H	-0.8007
137	G	H	-0.7736
138	G	H	-0.5906
139	T	H	-0.4445
140	A	H	-0.3591
141	A	H	0.0000
142	L	H	0.0000
143	G	H	0.0000
144	C	H	0.0000
145	L	H	0.0000
146	V	H	0.0000
147	K	H	-0.4315
148	D	H	-0.6768
149	Y	H	0.0000
150	F	H	0.0000
151	P	H	-1.0086
152	E	H	-1.2792
153	P	H	-1.3227
154	V	H	0.0000
155	T	H	-0.9596
156	V	H	-0.4680
157	S	H	-0.4065
158	W	H	0.0000
159	N	H	-0.7682
160	S	H	-0.6400
161	G	H	-0.6348
162	A	H	-0.3122
163	L	H	-0.1031
164	T	H	-0.2398
165	S	H	-0.2222
166	G	H	-0.3384
167	V	H	0.0769
168	H	H	-0.2983
169	T	H	0.0160
170	F	H	0.0000
171	P	H	-0.4324
172	A	H	-0.1851
173	V	H	0.0000
174	L	H	0.6299
175	Q	H	0.0665
176	S	H	-0.1319
177	S	H	-0.2320
178	G	H	-0.0465
179	L	H	-0.2424
180	Y	H	0.0000
181	S	H	0.0000
182	L	H	0.0000
183	S	H	0.0000
184	S	H	0.0000
185	V	H	0.0000
186	V	H	0.0000
187	T	H	-0.1095
188	V	H	0.0000
189	P	H	-0.6286
190	S	H	0.0000
191	S	H	-0.7818
192	S	H	-0.7774
193	L	H	0.0000
194	G	H	-1.2434
195	T	H	-1.1115
196	Q	H	-1.5186
197	T	H	-1.2165
198	Y	H	0.0000
199	I	H	-1.1679
200	C	H	0.0000
201	N	H	-1.2880
202	V	H	0.0000
203	N	H	-2.2744
204	H	H	0.0000
205	K	H	-2.9653
206	P	H	-1.8080
207	S	H	-1.8799
208	N	H	-2.6231
209	T	H	-2.1636
210	K	H	-2.6531
211	V	H	-1.3964
212	D	H	-1.8954
213	K	H	-1.6459
214	K	H	-2.3546
215	V	H	0.0000
216	E	H	-2.5725
217	P	H	-1.5474
218	K	H	-1.3844
219	S	H	-1.3162
220	C	H	-1.8848
221	D	H	-2.8924
222	K	H	-2.7991
223	T	H	-2.1472
224	H	H	-1.8362
225	T	H	-1.3599
226	S	H	-0.6577
227	A	H	-1.1408
228	A	H	-1.2271