Project name: H3807 [mutate: LL9I]

Chain sequence(s)	H: VQLVQSGAEVKKPGASVKVSCKASGYTFTNYWLGWIKQAPGQGLEWIGDIYPGGDYIVYNEKFKGKATLTADTSISTAYMELSRLRSDDTAVYYCARPNLPKDHWGQGTTVTVS; L: DIVMTQSPLSLPVTPGEPASISCRSSKSLLHSQGITYLYWYLQKPGQSPQLLIYQVSNLASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCAQYLELPWTFGGGTKVEIK
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LL9I
Energy difference between WT (input) and mutated protein (by FoldX)	0.315854 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.3938
• Maximal score value: 1.695
• Average score: -0.6047
• Total score value: -136.6632

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	D	L	-1.4973
2	I	L	0.0000
3	V	L	0.8122
4	M	L	0.0000
5	T	L	-0.4281
6	Q	L	-0.1611
7	S	L	-0.1457
8	P	L	0.5860
9	I	L	1.6950	mutated: LL9I
10	S	L	0.3964
11	L	L	0.0924
12	P	L	-0.9726
13	V	L	0.0000
14	T	L	-1.8051
15	P	L	-2.0528
16	G	L	-2.1597
17	E	L	-2.8636
18	P	L	-2.4320
19	A	L	-1.3636
20	S	L	-0.7915
21	I	L	0.0000
22	S	L	-0.9574
23	C	L	0.0000
24	R	L	-1.8755
25	S	L	0.0000
26	S	L	-0.9357
27	K	L	-1.4576
28	S	L	-0.6757
29	L	L	0.0000
30	L	L	1.0122
30A	H	L	-0.0338
30B	S	L	-0.5917
30C	Q	L	-1.1720
30D	G	L	-0.4738
30E	I	L	0.3593
31	T	L	0.4373
32	Y	L	0.4879
33	L	L	0.0000
34	Y	L	0.0000
35	W	L	0.0000
36	Y	L	0.0000
37	L	L	0.0000
38	Q	L	0.0000
39	K	L	-1.2133
40	P	L	-0.9262
41	G	L	-1.3553
42	Q	L	-1.8896
43	S	L	-1.3610
44	P	L	0.0000
45	Q	L	-1.3973
46	L	L	-0.4406
47	L	L	0.0000
48	I	L	0.0000
49	Y	L	-0.0255
50	Q	L	-0.2374
51	V	L	-0.2023
52	S	L	-0.6240
53	N	L	-0.8151
54	L	L	-0.3457
55	A	L	0.0000
56	S	L	-0.3306
57	G	L	-0.6149
58	V	L	-0.6093
59	P	L	-1.0797
60	D	L	-1.9814
61	R	L	-2.1595
62	F	L	0.0000
63	S	L	-1.2128
64	G	L	-0.8253
65	S	L	-1.0776
66	G	L	-1.1690
67	S	L	-0.7892
68	G	L	-0.6469
69	T	L	-1.3418
70	D	L	-1.9656
71	F	L	0.0000
72	T	L	-1.0805
73	L	L	0.0000
74	K	L	-1.8871
75	I	L	0.0000
76	S	L	-2.5272
77	R	L	-3.3938
78	V	L	0.0000
79	E	L	-2.8784
80	A	L	-1.5252
81	E	L	-2.1599
82	D	L	0.0000
83	V	L	-0.4669
84	G	L	0.0000
85	V	L	0.1384
86	Y	L	0.0000
87	Y	L	0.0000
88	C	L	0.0000
89	A	L	0.0000
90	Q	L	0.0000
91	Y	L	0.0000
92	L	L	0.3754
93	E	L	-0.8116
94	L	L	0.0857
95	P	L	-0.7196
96	W	L	0.0000
97	T	L	-0.2731
98	F	L	-0.0966
99	G	L	0.0000
100	G	L	-0.5520
101	G	L	0.0000
102	T	L	0.0000
103	K	L	-0.1420
104	V	L	0.0000
105	E	L	-1.5114
106	I	L	-1.1340
107	K	L	-1.8727
2	V	H	0.4406
3	Q	H	-0.7715
4	L	H	0.0000
5	V	H	-0.0124
6	Q	H	0.0000
7	S	H	-0.5765
8	G	H	-0.6927
9	A	H	-0.0781
10	E	H	-0.2245
11	V	H	0.9366
12	K	H	-0.9135
13	K	H	-2.1775
14	P	H	-1.9335
15	G	H	-1.9702
16	A	H	-1.6285
17	S	H	-1.6875
18	V	H	0.0000
19	K	H	-2.2145
20	V	H	0.0000
21	S	H	-0.6571
22	C	H	0.0000
23	K	H	-0.7759
24	A	H	0.0000
25	S	H	-0.1966
26	G	H	-0.1409
27	Y	H	0.0519
28	T	H	-0.2172
29	F	H	0.0000
30	T	H	-0.9040
31	N	H	-1.1038
32	Y	H	-0.0206
33	W	H	0.0384
34	L	H	0.0000
35	G	H	0.0000
36	W	H	0.0000
37	I	H	0.0000
38	K	H	-0.8725
39	Q	H	0.0000
40	A	H	-1.1141
41	P	H	-0.8328
42	G	H	-1.2193
43	Q	H	-1.8482
44	G	H	-1.3282
45	L	H	0.0000
46	E	H	-2.0416
47	W	H	0.0000
48	I	H	0.0000
49	G	H	0.0000
50	D	H	0.0000
51	I	H	0.0000
52	Y	H	0.0391
52A	P	H	0.0000
53	G	H	-1.1294
54	G	H	-1.0615
55	D	H	-1.1908
56	Y	H	0.8077
57	I	H	1.1320
58	V	H	0.6168
59	Y	H	-0.7320
60	N	H	-2.1026
61	E	H	-3.2316
62	K	H	-3.2356
63	F	H	0.0000
64	K	H	-3.0953
65	G	H	-2.2153
66	K	H	-2.0536
67	A	H	0.0000
68	T	H	-0.9360
69	L	H	0.0000
70	T	H	-0.3738
71	A	H	-0.6550
72	D	H	-0.6146
73	T	H	-0.3542
74	S	H	0.2855
75	I	H	0.9998
76	S	H	0.0880
77	T	H	0.0000
78	A	H	0.0000
79	Y	H	-0.7381
80	M	H	0.0000
81	E	H	-1.8377
82	L	H	0.0000
82A	S	H	-1.9216
82B	R	H	-2.7429
82C	L	H	0.0000
83	R	H	-3.3367
84	S	H	-2.2296
85	D	H	-2.5055
86	D	H	0.0000
87	T	H	-0.9112
88	A	H	0.0000
89	V	H	-0.0469
90	Y	H	0.0000
91	Y	H	0.0000
92	C	H	0.0000
93	A	H	0.0000
94	R	H	-0.3964
95	P	H	0.0000
96	N	H	-0.7737
97	L	H	0.2408
98	P	H	-0.2509
99	K	H	0.0000
101	D	H	-1.0276
102	H	H	-0.9900
103	W	H	-0.7534
104	G	H	0.0000
105	Q	H	-1.4815
106	G	H	0.0000
107	T	H	0.0000
108	T	H	-0.0735
109	V	H	0.0000
110	T	H	-0.3201
111	V	H	0.0000
112	S	H	-0.8311