Project name: 180810TfusB_Aggrescan_Static [mutate: WA20R, AA25R, VA26R, AA28R, VA101R, VA103R, VA126R, YA128R] [mutate: RA20D, RA25D, RA26D, RA28D, RA101D, RA103D, RA126D, RA128D]

Chain sequence(s)	A: MSENVVLQRSNVRLSWRTKRAARCRRGRARLLARKPPERIRATLLRLRGEVRPATYEEAKAARDAVLAVSLRCAGLRACLQRSLAIALLCRMRGTWATWCRGRPRRPPFIGHAWVEAEGRLVEEGRGRDYFSRLITVD
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	RA20D, RA25D, RA26D, RA28D, RA101D, RA103D, RA126D, RA128D
Energy difference between WT (input) and mutated protein (by FoldX)	2.07376 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.4211
• Maximal score value: 1.7229
• Average score: -0.7169
• Total score value: -98.9328

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	M	A	-1.8296
2	S	A	-1.3768
3	E	A	-3.1428
4	N	A	0.0000
5	V	A	0.0000
6	V	A	-1.1639
7	L	A	0.0000
8	Q	A	-1.9342
9	R	A	-2.4777
10	S	A	-1.8365
11	N	A	-2.1239
12	V	A	-0.8866
13	R	A	0.0000
14	L	A	-0.4989
15	S	A	-0.4783
16	W	A	-0.0403
17	R	A	-2.4659
18	T	A	-2.0391
19	K	A	0.0000
20	D	A	-3.4211	mutated: RA20D
21	A	A	-2.1878
22	A	A	0.0000
23	R	A	0.0000
24	C	A	-1.6047
25	D	A	-3.1584	mutated: RA25D
26	D	A	-2.7863	mutated: RA26D
27	G	A	0.0000
28	D	A	-1.5037	mutated: RA28D
29	A	A	-1.1632
30	R	A	-1.4282
31	L	A	0.0000
32	L	A	-0.4917
33	A	A	-0.9617
34	R	A	-2.4567
35	K	A	-2.5450
36	P	A	-1.4884
37	P	A	-1.3491
38	E	A	-1.7401
39	R	A	0.0000
40	I	A	0.0000
41	R	A	-1.6883
42	A	A	-0.4984
43	T	A	0.0000
44	L	A	-0.7183
45	L	A	-0.3851
46	R	A	-1.1973
47	L	A	0.0000
48	R	A	-1.9855
49	G	A	-1.3606
50	E	A	-1.5220
51	V	A	-0.7285
52	R	A	-1.9225
53	P	A	-0.9863
54	A	A	-0.4561
55	T	A	-0.5121
56	Y	A	-0.0799
57	E	A	-2.0883
58	E	A	0.0000
59	A	A	-1.2240
60	K	A	-2.4260
61	A	A	-1.8692
62	A	A	0.0000
63	R	A	-1.6019
64	D	A	-1.6374
65	A	A	0.0000
66	V	A	0.0000
67	L	A	-0.0976
68	A	A	-0.0638
69	V	A	-0.0698
70	S	A	0.0000
71	L	A	0.4478
72	R	A	-1.1231
73	C	A	-0.8378
74	A	A	-0.6556
75	G	A	-1.1749
76	L	A	0.0000
77	R	A	-1.5978
78	A	A	-0.1404
79	C	A	-0.0166
80	L	A	0.4929
81	Q	A	0.0000
82	R	A	0.0000
83	S	A	0.0000
84	L	A	0.0000
85	A	A	0.0000
86	I	A	0.0000
87	A	A	0.0000
88	L	A	0.0000
89	L	A	0.0000
90	C	A	0.0000
91	R	A	-0.0945
92	M	A	-0.2011
93	R	A	0.0000
94	G	A	-0.4698
95	T	A	0.0000
96	W	A	0.2338
97	A	A	0.0000
98	T	A	0.0000
99	W	A	0.1639
100	C	A	0.0000
101	D	A	0.1857	mutated: RA101D
102	G	A	0.0000
103	D	A	-0.7780	mutated: RA103D
104	P	A	-1.2129
105	R	A	-1.5890
106	R	A	-0.9029
107	P	A	-0.2051
108	P	A	0.1883
109	F	A	1.0669
110	I	A	0.7927
111	G	A	0.2310
112	H	A	0.0000
113	A	A	0.0000
114	W	A	0.0117
115	V	A	0.0000
116	E	A	0.0000
117	A	A	0.0000
118	E	A	-1.0872
119	G	A	-1.0454
120	R	A	-0.5689
121	L	A	0.0526
122	V	A	-0.0859
123	E	A	-1.3390
124	E	A	0.0000
125	G	A	-1.0535
126	D	A	-2.2195	mutated: RA126D
127	G	A	0.0000
128	D	A	-2.9572	mutated: RA128D
129	D	A	-2.6905
130	Y	A	-1.2790
131	F	A	0.0000
132	S	A	-1.4967
133	R	A	-1.3996
134	L	A	0.7538
135	I	A	1.7229
136	T	A	0.4352
137	V	A	-0.1599
138	D	A	-1.6307