Project name: fr-GFP [mutate: VA11D]

Chain sequence(s)	A: KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITH
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA11D
Energy difference between WT (input) and mutated protein (by FoldX)	1.07915 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -4.2779
• Maximal score value: 0.4967
• Average score: -1.0896
• Total score value: -246.2386

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
3	K	A	-2.4362
4	G	A	0.0000
5	E	A	-2.4090
6	E	A	-2.4823
7	L	A	-1.2773
8	F	A	0.0000
9	T	A	-1.0006
10	G	A	-1.2462
11	D	A	-2.3202	mutated: VA11D
12	V	A	0.0000
13	P	A	-1.8837
14	I	A	0.0000
15	L	A	-1.3996
16	V	A	0.0000
17	E	A	-2.6162
18	L	A	0.0000
19	D	A	-3.6081
20	G	A	0.0000
21	D	A	-2.8114
22	V	A	0.0000
23	N	A	-2.1016
24	G	A	-1.7080
25	H	A	-2.2368
26	K	A	-3.0008
27	F	A	0.0000
28	S	A	-1.8934
29	V	A	0.0000
30	S	A	-1.2801
31	G	A	0.0000
32	E	A	-2.3473
33	G	A	-1.8392
34	E	A	-2.6420
35	G	A	0.0000
36	D	A	-1.4568
37	A	A	0.0000
38	T	A	-0.3719
39	Y	A	0.4967
40	G	A	0.0000
41	K	A	-0.8543
42	L	A	0.0000
43	T	A	-0.8864
44	L	A	0.0000
45	K	A	-1.2182
46	F	A	0.0000
47	I	A	-0.9190
48	C	A	0.0000
49	T	A	-1.0341
50	T	A	-1.3311
51	G	A	-1.6471
52	K	A	-2.2716
53	L	A	0.0000
54	P	A	-1.2309
55	V	A	0.0000
56	P	A	-0.7448
57	W	A	0.0000
58	P	A	0.0000
59	T	A	0.0000
60	L	A	0.0000
61	V	A	0.0000
62	T	A	0.0001
63	T	A	0.0000
64	L	A	0.0000
68	V	A	0.0079
69	Q	A	-0.1839
70	C	A	0.0000
71	F	A	0.0000
72	S	A	0.0000
73	R	A	-1.3714
74	Y	A	0.0000
75	P	A	-1.9126
76	D	A	-2.8496
77	H	A	-2.1242
78	M	A	0.0000
79	K	A	-2.8500
80	R	A	-2.8540
81	H	A	-1.7270
82	D	A	0.0000
83	F	A	0.0000
84	F	A	0.0000
85	K	A	0.0000
86	S	A	-1.2084
87	A	A	0.0000
88	M	A	0.0000
89	P	A	-1.6218
90	E	A	-2.2180
91	G	A	0.0000
92	Y	A	0.0000
93	V	A	-0.8663
94	Q	A	0.0000
95	E	A	-2.3539
96	R	A	0.0000
97	T	A	-1.0670
98	I	A	0.0000
99	S	A	-1.2960
100	F	A	0.0000
101	K	A	-2.5427
102	D	A	-2.9337
103	D	A	-2.8715
104	G	A	0.0000
105	N	A	-1.5683
106	Y	A	0.0000
107	K	A	-2.5624
108	T	A	0.0000
109	R	A	-3.4394
110	A	A	0.0000
111	E	A	-2.3202
112	V	A	0.0000
113	K	A	-1.6673
114	F	A	-1.8720
115	E	A	-2.6836
116	G	A	-2.2569
117	D	A	-2.3903
118	T	A	-1.9703
119	L	A	0.0000
120	V	A	0.0000
121	N	A	0.0000
122	R	A	-3.4270
123	I	A	0.0000
124	E	A	-4.2779
125	L	A	0.0000
126	K	A	-3.1114
127	G	A	0.0000
128	I	A	-1.2424
129	D	A	-2.2779
130	F	A	0.0000
131	K	A	-3.9555
132	E	A	-4.0006
133	D	A	-3.6539
134	G	A	-2.8630
135	N	A	-2.3218
136	I	A	0.0000
137	L	A	-2.0559
138	G	A	-2.3871
139	H	A	-2.2799
140	K	A	-2.6113
141	L	A	-1.9367
142	E	A	-2.3872
143	Y	A	-1.0241
144	N	A	-0.7127
145	Y	A	-0.8071
146	N	A	-1.0167
147	S	A	-1.0652
148	H	A	0.0000
149	N	A	-1.4592
150	V	A	0.0000
151	Y	A	-0.0400
152	I	A	0.0000
153	T	A	-1.1445
154	A	A	-1.7207
155	D	A	-2.2292
156	K	A	-3.2073
157	Q	A	-3.1666
158	K	A	-3.2467
159	N	A	-2.8730
160	G	A	0.0000
161	I	A	0.0000
162	K	A	-1.0801
163	A	A	0.0000
164	N	A	-1.2651
165	F	A	0.0000
166	K	A	-1.8271
167	I	A	0.0000
168	R	A	-1.1564
169	H	A	0.0000
170	N	A	-1.4604
171	I	A	0.0000
172	E	A	-3.4499
173	D	A	-3.0634
174	G	A	-1.8715
175	S	A	-0.8002
176	V	A	0.1146
177	Q	A	0.0000
178	L	A	-0.9215
179	A	A	0.0000
180	D	A	-1.4493
181	H	A	0.0000
182	Y	A	-0.3596
183	Q	A	0.0000
184	Q	A	-1.1028
185	N	A	0.0000
186	T	A	-0.7914
187	P	A	-0.9319
188	I	A	-0.4177
189	G	A	-1.3726
190	D	A	-2.1764
191	G	A	-1.5679
192	P	A	-0.8324
193	V	A	-0.1261
194	L	A	-0.2338
195	L	A	-0.6429
196	P	A	0.0000
197	D	A	-2.4703
198	N	A	-1.8734
199	H	A	0.0000
200	Y	A	-0.3017
201	L	A	0.0000
202	S	A	-0.7184
203	T	A	-0.9461
204	Q	A	-1.2941
205	S	A	-0.5056
206	A	A	0.0239
207	L	A	-0.0777
208	S	A	-0.7222
209	K	A	-2.0055
210	D	A	-2.1408
211	P	A	-1.8366
212	N	A	-2.4864
213	E	A	-2.7188
214	K	A	-3.3438
215	R	A	-3.3663
216	D	A	-2.2775
217	H	A	0.0000
218	M	A	0.0000
219	V	A	-0.3507
220	L	A	0.0000
221	L	A	0.3888
222	E	A	-0.1818
223	F	A	0.0445
224	V	A	0.0000
225	T	A	-0.5207
226	A	A	0.0000
227	A	A	-0.4247
228	G	A	-0.6622
229	I	A	-0.7183
230	T	A	-0.5700
231	H	A	-1.3097