| Chain sequence(s) |
A: LPPGPTPLPVIGNILQIDIKDVSKSLTNLSKIYGPVFTLYFGLERMVVLHGYEVVKEALIDLGEEFSGRGHFPLAERANRGFGIVFSNGKRWKEIRRFSLMTLRNFGMGKRSIEDRVQEEARCLVEELRKTKASPCDPTFILGCAPCNVICSIIFQKRFDYKDQQFLNLMEKLNENIRIVSTPWIQICNNFPTIIDYFPGTHNKLLKNLAFMESDILEKVKEHQESMDINNPRDFIDCFLIKMEKEKQNQQSEFTIENLVITAADLLGAGTETTSTTLRYALLLLLKHPEVTAKVQEEIERVVGRNRSPCMQDRGHMPYTDAVVHEVQRYIDLIPTSLPHAVTCDVKFRNYLIPKGTTILTSLTSVLHDNKEFPNPEMFDPRHFLDEGGNFKKSNYFMPFSAGKRICVGEGLARMELFLFLTFILQNFNLKSLIDPKDLDTTPVVNGFASVPPFYQLCFIPIHH |
| Distance of aggregation | 10 Å |
| Dynamic mode | No |
| Mutated residues | VA331I |
| Energy difference between WT (input) and mutated protein (by FoldX) | 0.73778 kcal/mol
Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 29 | L | A | 0.8394 | |
| 30 | P | A | 0.0000 | |
| 31 | P | A | 0.4032 | |
| 32 | G | A | 0.3009 | |
| 33 | P | A | 0.2900 | |
| 34 | T | A | -0.0191 | |
| 35 | P | A | 1.0106 | |
| 36 | L | A | 1.8077 | |
| 37 | P | A | 1.1764 | |
| 38 | V | A | 2.6047 | |
| 39 | I | A | 2.5727 | |
| 40 | G | A | 0.0000 | |
| 41 | N | A | 0.0000 | |
| 42 | I | A | 1.9372 | |
| 43 | L | A | 1.6464 | |
| 44 | Q | A | -0.0507 | |
| 45 | I | A | 0.0000 | |
| 46 | D | A | -1.7016 | |
| 47 | I | A | -0.6065 | |
| 48 | K | A | -1.8123 | |
| 49 | D | A | -1.2374 | |
| 50 | V | A | -0.8233 | |
| 51 | S | A | -0.9380 | |
| 52 | K | A | -2.1875 | |
| 53 | S | A | 0.0000 | |
| 54 | L | A | 0.0000 | |
| 55 | T | A | -1.2320 | |
| 56 | N | A | -1.6000 | |
| 57 | L | A | 0.0000 | |
| 58 | S | A | -0.6577 | |
| 59 | K | A | -1.0258 | |
| 60 | I | A | 1.0630 | |
| 61 | Y | A | 0.5875 | |
| 62 | G | A | -0.0659 | |
| 63 | P | A | -0.4402 | |
| 64 | V | A | 0.0000 | |
| 65 | F | A | 0.0000 | |
| 66 | T | A | 0.0000 | |
| 67 | L | A | 0.0000 | |
| 68 | Y | A | 0.8411 | |
| 69 | F | A | 1.2115 | |
| 70 | G | A | 1.3977 | |
| 71 | L | A | 1.6637 | |
| 72 | E | A | 0.2122 | |
| 73 | R | A | -0.3068 | |
| 74 | M | A | 0.0000 | |
| 75 | V | A | 0.0000 | |
| 76 | V | A | 0.0000 | |
| 77 | L | A | 0.0000 | |
| 78 | H | A | -0.6640 | |
| 79 | G | A | -0.9126 | |
| 80 | Y | A | -1.6236 | |
| 81 | E | A | -2.5033 | |
| 82 | V | A | 0.0000 | |
| 83 | V | A | 0.0000 | |
| 84 | K | A | -2.0892 | |
| 85 | E | A | -2.7527 | |
| 86 | A | A | 0.0000 | |
| 87 | L | A | 0.0000 | |
| 88 | I | A | -0.4797 | |
| 89 | D | A | -1.9488 | |
| 90 | L | A | -1.2938 | |
| 91 | G | A | 0.0000 | |
| 92 | E | A | -2.2069 | |
| 93 | E | A | -1.9430 | |
| 94 | F | A | 0.0000 | |
| 95 | S | A | 0.0000 | |
| 96 | G | A | 0.0000 | |
| 97 | R | A | -0.5210 | |
| 98 | G | A | 0.0000 | |
| 99 | H | A | -0.8422 | |
| 100 | F | A | -0.6337 | |
| 101 | P | A | 0.0000 | |
| 102 | L | A | 0.0000 | |
| 103 | A | A | 0.0000 | |
| 104 | E | A | -2.1170 | |
| 105 | R | A | -1.8566 | |
| 106 | A | A | -1.3074 | |
| 107 | N | A | -1.5763 | |
| 108 | R | A | -2.4936 | |
| 109 | G | A | -1.5492 | |
| 110 | F | A | -1.0472 | |
| 111 | G | A | 0.0000 | |
| 112 | I | A | 0.0000 | |
| 113 | V | A | 0.3818 | |
| 114 | F | A | -0.2710 | |
| 115 | S | A | 0.0000 | |
| 116 | N | A | -2.1412 | |
| 117 | G | A | -2.3073 | |
| 118 | K | A | -2.7673 | |
| 119 | R | A | -2.3969 | |
| 120 | W | A | 0.0000 | |
| 121 | K | A | -3.5454 | |
| 122 | E | A | -2.7739 | |
| 123 | I | A | 0.0000 | |
| 124 | R | A | -1.8650 | |
| 125 | R | A | -2.2205 | |
| 126 | F | A | 0.0000 | |
| 127 | S | A | 0.0000 | |
| 128 | L | A | -0.2264 | |
| 129 | M | A | -0.2419 | |
| 130 | T | A | 0.0000 | |
| 131 | L | A | -0.3702 | |
| 132 | R | A | -1.2506 | |
| 133 | N | A | -1.1804 | |
| 134 | F | A | 0.1965 | |
| 135 | G | A | -0.3362 | |
| 136 | M | A | 0.0000 | |
| 137 | G | A | -1.6062 | |
| 138 | K | A | -2.4786 | |
| 139 | R | A | -2.7587 | |
| 140 | S | A | 0.0000 | |
| 141 | I | A | 0.0000 | |
| 142 | E | A | -1.3985 | |
| 143 | D | A | -2.2737 | |
| 144 | R | A | -2.2211 | |
| 145 | V | A | 0.0000 | |
| 146 | Q | A | -1.9985 | |
| 147 | E | A | -2.9326 | |
| 148 | E | A | 0.0000 | |
| 149 | A | A | 0.0000 | |
| 150 | R | A | -3.0296 | |
| 151 | C | A | -2.3553 | |
| 152 | L | A | 0.0000 | |
| 153 | V | A | 0.0000 | |
| 154 | E | A | -3.1925 | |
| 155 | E | A | -2.7242 | |
| 156 | L | A | 0.0000 | |
| 157 | R | A | -3.0801 | |
| 158 | K | A | -3.3796 | |
| 159 | T | A | -2.4407 | |
| 160 | K | A | -2.6673 | |
| 161 | A | A | -1.4780 | |
| 162 | S | A | -1.0943 | |
| 163 | P | A | -0.8800 | |
| 164 | C | A | -0.9561 | |
| 165 | D | A | -1.3109 | |
| 166 | P | A | 0.0000 | |
| 167 | T | A | 0.1727 | |
| 168 | F | A | 0.8795 | |
| 169 | I | A | 0.0000 | |
| 170 | L | A | 0.0000 | |
| 171 | G | A | 0.1467 | |
| 172 | C | A | 0.0000 | |
| 173 | A | A | 0.0000 | |
| 174 | P | A | 0.0000 | |
| 175 | C | A | 0.0000 | |
| 176 | N | A | 0.0000 | |
| 177 | V | A | 0.0000 | |
| 178 | I | A | 0.0000 | |
| 179 | C | A | 0.0000 | |
| 180 | S | A | 0.0000 | |
| 181 | I | A | 0.0000 | |
| 182 | I | A | 0.0000 | |
| 183 | F | A | 0.0000 | |
| 184 | Q | A | -2.1529 | |
| 185 | K | A | -2.4427 | |
| 186 | R | A | -1.8428 | |
| 187 | F | A | -1.6867 | |
| 188 | D | A | -2.4416 | |
| 189 | Y | A | 0.0000 | |
| 190 | K | A | -2.8420 | |
| 191 | D | A | -2.8459 | |
| 192 | Q | A | -2.5988 | |
| 193 | Q | A | -2.1946 | |
| 194 | F | A | 0.0000 | |
| 195 | L | A | -2.0499 | |
| 196 | N | A | -2.4542 | |
| 197 | L | A | 0.0000 | |
| 198 | M | A | 0.0000 | |
| 199 | E | A | -2.7080 | |
| 200 | K | A | -1.6213 | |
| 201 | L | A | 0.0000 | |
| 202 | N | A | -2.1808 | |
| 203 | E | A | -2.0115 | |
| 204 | N | A | 0.0000 | |
| 205 | I | A | -0.7601 | |
| 206 | R | A | -1.9050 | |
| 207 | I | A | -0.9508 | |
| 208 | V | A | -0.1325 | |
| 209 | S | A | -0.4862 | |
| 210 | T | A | -0.4909 | |
| 211 | P | A | -0.1750 | |
| 212 | W | A | 0.2657 | |
| 213 | I | A | 0.0000 | |
| 214 | Q | A | -0.1942 | |
| 215 | I | A | 0.8317 | |
| 216 | C | A | 0.0000 | |
| 217 | N | A | 0.1849 | |
| 218 | N | A | 0.0000 | |
| 219 | F | A | 1.2522 | |
| 220 | P | A | 0.3959 | |
| 221 | T | A | 0.4604 | |
| 222 | I | A | 0.9046 | |
| 223 | I | A | 0.0000 | |
| 224 | D | A | -0.5706 | |
| 225 | Y | A | 1.3598 | |
| 226 | F | A | 1.7654 | |
| 227 | P | A | 0.0090 | |
| 228 | G | A | -0.4723 | |
| 229 | T | A | -0.3658 | |
| 230 | H | A | 0.0000 | |
| 231 | N | A | -1.3490 | |
| 232 | K | A | -2.1502 | |
| 233 | L | A | 0.0000 | |
| 234 | L | A | -0.6462 | |
| 235 | K | A | -1.1474 | |
| 236 | N | A | 0.0000 | |
| 237 | L | A | 0.0623 | |
| 238 | A | A | 0.3037 | |
| 239 | F | A | 1.3249 | |
| 240 | M | A | 0.0000 | |
| 241 | E | A | -0.4954 | |
| 242 | S | A | -0.4596 | |
| 243 | D | A | -1.4315 | |
| 244 | I | A | 0.0000 | |
| 245 | L | A | -1.5078 | |
| 246 | E | A | -3.1090 | |
| 247 | K | A | -2.7120 | |
| 248 | V | A | 0.0000 | |
| 249 | K | A | -3.5333 | |
| 250 | E | A | -3.8054 | |
| 251 | H | A | 0.0000 | |
| 252 | Q | A | -2.9118 | |
| 253 | E | A | -3.4115 | |
| 254 | S | A | -2.1662 | |
| 255 | M | A | -2.0570 | |
| 256 | D | A | -2.2399 | |
| 257 | I | A | -0.8147 | |
| 258 | N | A | -1.6443 | |
| 259 | N | A | -1.9361 | |
| 260 | P | A | -1.6189 | |
| 261 | R | A | -1.9610 | |
| 262 | D | A | 0.0000 | |
| 263 | F | A | 0.0000 | |
| 264 | I | A | 0.0000 | |
| 265 | D | A | 0.0000 | |
| 266 | C | A | -0.2817 | |
| 267 | F | A | 0.0000 | |
| 268 | L | A | 0.0000 | |
| 269 | I | A | -1.2701 | |
| 270 | K | A | -1.5141 | |
| 271 | M | A | -1.9785 | |
| 272 | E | A | -2.9028 | |
| 273 | K | A | -3.5586 | |
| 274 | E | A | -3.7194 | |
| 275 | K | A | -4.2406 | |
| 276 | Q | A | -3.7398 | |
| 277 | N | A | -3.6455 | |
| 278 | Q | A | -3.0808 | |
| 279 | Q | A | -2.7077 | |
| 280 | S | A | -2.4596 | |
| 281 | E | A | -1.5088 | |
| 282 | F | A | 0.0000 | |
| 283 | T | A | -0.6286 | |
| 284 | I | A | -0.4499 | |
| 285 | E | A | -1.5144 | |
| 286 | N | A | 0.0000 | |
| 287 | L | A | 0.0000 | |
| 288 | V | A | 0.0000 | |
| 289 | I | A | 0.0000 | |
| 290 | T | A | 0.0000 | |
| 291 | A | A | 0.0000 | |
| 292 | A | A | -0.1097 | |
| 293 | D | A | -0.0325 | |
| 294 | L | A | 0.0857 | |
| 295 | L | A | 0.0000 | |
| 296 | G | A | -0.2800 | |
| 297 | A | A | -0.0990 | |
| 298 | G | A | -0.2602 | |
| 299 | T | A | 0.0000 | |
| 300 | E | A | -0.7230 | |
| 301 | T | A | -0.3393 | |
| 302 | T | A | 0.0000 | |
| 303 | S | A | -0.1948 | |
| 304 | T | A | 0.0000 | |
| 305 | T | A | 0.0000 | |
| 306 | L | A | 0.0000 | |
| 307 | R | A | -0.0393 | |
| 308 | Y | A | 0.0000 | |
| 309 | A | A | 0.0000 | |
| 310 | L | A | 0.0000 | |
| 311 | L | A | 0.0000 | |
| 312 | L | A | 0.0000 | |
| 313 | L | A | 0.0000 | |
| 314 | L | A | 0.0000 | |
| 315 | K | A | -2.3579 | |
| 316 | H | A | -1.3466 | |
| 317 | P | A | -1.7492 | |
| 318 | E | A | -2.0294 | |
| 319 | V | A | 0.0000 | |
| 320 | T | A | 0.0000 | |
| 321 | A | A | -1.6694 | |
| 322 | K | A | -2.4232 | |
| 323 | V | A | 0.0000 | |
| 324 | Q | A | -2.3571 | |
| 325 | E | A | -3.4754 | |
| 326 | E | A | 0.0000 | |
| 327 | I | A | 0.0000 | |
| 328 | E | A | -3.9649 | |
| 329 | R | A | -3.4342 | |
| 330 | V | A | -1.7500 | |
| 331 | I | A | 0.0000 | mutated: VA331I |
| 332 | G | A | -3.0491 | |
| 333 | R | A | -3.6764 | |
| 334 | N | A | -2.6160 | |
| 335 | R | A | -2.0204 | |
| 336 | S | A | -1.3634 | |
| 337 | P | A | 0.0000 | |
| 338 | C | A | -0.6240 | |
| 339 | M | A | -0.8348 | |
| 340 | Q | A | -1.5955 | |
| 341 | D | A | 0.0000 | |
| 342 | R | A | -1.4796 | |
| 343 | G | A | -1.5178 | |
| 344 | H | A | -1.8819 | |
| 345 | M | A | 0.0000 | |
| 346 | P | A | -1.0468 | |
| 347 | Y | A | -0.8156 | |
| 348 | T | A | 0.0000 | |
| 349 | D | A | 0.0000 | |
| 350 | A | A | 0.0000 | |
| 351 | V | A | 0.0000 | |
| 352 | V | A | 0.0000 | |
| 353 | H | A | 0.0000 | |
| 354 | E | A | 0.0000 | |
| 355 | V | A | 0.0000 | |
| 356 | Q | A | 0.0000 | |
| 357 | R | A | 0.0000 | |
| 358 | Y | A | -0.2305 | |
| 359 | I | A | 0.0000 | |
| 360 | D | A | 0.0000 | |
| 361 | L | A | 0.0000 | |
| 362 | I | A | 0.6107 | |
| 363 | P | A | 0.0000 | |
| 364 | T | A | 0.3654 | |
| 365 | S | A | 0.0000 | |
| 366 | L | A | 0.4848 | |
| 367 | P | A | 0.0284 | |
| 368 | H | A | 0.0000 | |
| 369 | A | A | 0.0000 | |
| 370 | V | A | 0.0000 | |
| 371 | T | A | -1.6823 | |
| 372 | C | A | -2.1186 | |
| 373 | D | A | -2.8017 | |
| 374 | V | A | 0.0000 | |
| 375 | K | A | -2.2089 | |
| 376 | F | A | -1.6824 | |
| 377 | R | A | -2.3389 | |
| 378 | N | A | -1.8630 | |
| 379 | Y | A | -0.5653 | |
| 380 | L | A | -0.7055 | |
| 381 | I | A | 0.0000 | |
| 382 | P | A | -1.5817 | |
| 383 | K | A | -2.6088 | |
| 384 | G | A | -1.7582 | |
| 385 | T | A | 0.0000 | |
| 386 | T | A | 0.0000 | |
| 387 | I | A | 0.0000 | |
| 388 | L | A | 0.2045 | |
| 389 | T | A | 0.0000 | |
| 390 | S | A | 0.0000 | |
| 391 | L | A | 0.0000 | |
| 392 | T | A | -0.3548 | |
| 393 | S | A | -0.6370 | |
| 394 | V | A | 0.0000 | |
| 395 | L | A | 0.0000 | |
| 396 | H | A | -1.6562 | |
| 397 | D | A | -2.3441 | |
| 398 | N | A | -3.2695 | |
| 399 | K | A | -3.0714 | |
| 400 | E | A | -2.3385 | |
| 401 | F | A | 0.0000 | |
| 402 | P | A | -2.4993 | |
| 403 | N | A | -2.6917 | |
| 404 | P | A | 0.0000 | |
| 405 | E | A | -2.3239 | |
| 406 | M | A | -0.8930 | |
| 407 | F | A | 0.0000 | |
| 408 | D | A | -1.0757 | |
| 409 | P | A | 0.0000 | |
| 410 | R | A | -1.6793 | |
| 411 | H | A | -1.4524 | |
| 412 | F | A | 0.0000 | |
| 413 | L | A | -2.1348 | |
| 414 | D | A | -2.5346 | |
| 415 | E | A | -2.7444 | |
| 416 | G | A | -1.8623 | |
| 417 | G | A | -1.9528 | |
| 418 | N | A | -2.4472 | |
| 419 | F | A | -2.2063 | |
| 420 | K | A | -3.1335 | |
| 421 | K | A | -2.8654 | |
| 422 | S | A | -1.9087 | |
| 423 | N | A | -1.7208 | |
| 424 | Y | A | 0.0000 | |
| 425 | F | A | -0.5310 | |
| 426 | M | A | 0.0000 | |
| 427 | P | A | 0.2605 | |
| 428 | F | A | 0.3739 | |
| 429 | S | A | 0.2709 | |
| 430 | A | A | 0.0000 | |
| 431 | G | A | -0.4099 | |
| 432 | K | A | -1.2536 | |
| 433 | R | A | 0.0000 | |
| 434 | I | A | 0.4715 | |
| 435 | C | A | 0.5379 | |
| 436 | V | A | 0.4713 | |
| 437 | G | A | -0.1145 | |
| 438 | E | A | -0.5025 | |
| 439 | G | A | -0.8508 | |
| 440 | L | A | -0.5038 | |
| 441 | A | A | -0.2561 | |
| 442 | R | A | -0.3983 | |
| 443 | M | A | -0.2512 | |
| 444 | E | A | 0.0000 | |
| 445 | L | A | 0.0000 | |
| 446 | F | A | 0.0000 | |
| 447 | L | A | 0.0000 | |
| 448 | F | A | 0.0000 | |
| 449 | L | A | 0.0000 | |
| 450 | T | A | 0.0000 | |
| 451 | F | A | 0.0000 | |
| 452 | I | A | 0.0000 | |
| 453 | L | A | 0.0000 | |
| 454 | Q | A | -1.3558 | |
| 455 | N | A | -0.8141 | |
| 456 | F | A | 0.0000 | |
| 457 | N | A | -0.7176 | |
| 458 | L | A | -0.8724 | |
| 459 | K | A | -1.5405 | |
| 460 | S | A | -0.3319 | |
| 461 | L | A | 1.2285 | |
| 462 | I | A | 0.6274 | |
| 463 | D | A | -1.9491 | |
| 464 | P | A | -2.3805 | |
| 465 | K | A | -3.3282 | |
| 466 | D | A | -3.2819 | |
| 467 | L | A | -1.8022 | |
| 468 | D | A | -2.3641 | |
| 469 | T | A | 0.0000 | |
| 470 | T | A | -0.3343 | |
| 471 | P | A | 0.6343 | |
| 472 | V | A | 1.7049 | |
| 473 | V | A | 0.6382 | |
| 474 | N | A | -0.4586 | |
| 475 | G | A | -0.3163 | |
| 476 | F | A | 0.7093 | |
| 477 | A | A | 0.6237 | |
| 478 | S | A | 0.0000 | |
| 479 | V | A | 1.2764 | |
| 480 | P | A | 0.0000 | |
| 481 | P | A | 1.1533 | |
| 482 | F | A | 1.5946 | |
| 483 | Y | A | 0.4046 | |
| 484 | Q | A | -0.7727 | |
| 485 | L | A | 0.0000 | |
| 486 | C | A | -0.8794 | |
| 487 | F | A | 0.0000 | |
| 488 | I | A | -0.5483 | |
| 489 | P | A | -0.6546 | |
| 490 | I | A | -0.5031 | |
| 491 | H | A | -1.4977 | |
| 492 | H | A | -1.5937 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine