| Chain sequence(s) |
A: EAKTTLFCASDAKAYEKECHNVWATHACVPTDPNPQEVVLEQVTENFNMWKNDMVDQMQEDVISIWDQCLKPCVKLTNTSTLTQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGKGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEEEIVIRSKNLRDNAKIIIVQLQKSVEIVCTRPNNGDIRQAYCQISGRNWSEAVNQVKKKLKEHFPHKNISFQSSSGGDLEITTHSFNCGGEFFYCNTSGLFQDTISNATIMLPCRIKQIINMWQEVGKAIYAPPIKGQITCKSDITGLLLLRDGGDTTDNTEIFRPSGGDMRDNWRSELYKYKVVEIK |
| Distance of aggregation | 10 Å |
| Dynamic mode | No |
| Mutated residues | DA279A, NA280S, AA281V, KA282N, IA283T, SA363G, SA364E, GA365R, GA366S, DA367R, LA455N, RA456I, DA457G, GA458A, GA459P, EA466A, SA471E, DA474N |
| Energy difference between WT (input) and mutated protein (by FoldX) | 20.2367 kcal/mol
CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 47 | E | A | -2.3822 | |
| 48 | A | A | -1.7547 | |
| 49 | K | A | -2.3006 | |
| 50 | T | A | -1.1327 | |
| 51 | T | A | -0.2648 | |
| 52 | L | A | 0.0000 | |
| 53 | F | A | 1.1175 | |
| 54 | C | A | 0.0000 | |
| 55 | A | A | 0.0000 | |
| 56 | S | A | 0.0000 | |
| 57 | D | A | -1.9859 | |
| 58 | A | A | -1.0207 | |
| 59 | K | A | -1.2043 | |
| 60 | A | A | -0.3680 | |
| 61 | Y | A | 0.4492 | |
| 62 | E | A | -0.9331 | |
| 63 | K | A | -1.5615 | |
| 64 | E | A | 0.0000 | |
| 65 | C | A | 0.0000 | |
| 66 | H | A | 0.0000 | |
| 67 | N | A | 0.0000 | |
| 68 | V | A | -0.1891 | |
| 69 | W | A | -0.4431 | |
| 70 | A | A | 0.0000 | |
| 71 | T | A | -0.3819 | |
| 72 | H | A | -0.5756 | |
| 73 | A | A | 0.0382 | |
| 74 | C | A | 0.0000 | |
| 75 | V | A | 0.8950 | |
| 76 | P | A | -0.4112 | |
| 77 | T | A | -1.0171 | |
| 78 | D | A | -1.6178 | |
| 79 | P | A | -1.5990 | |
| 80 | N | A | -2.1775 | |
| 81 | P | A | -2.2504 | |
| 82 | Q | A | -2.1967 | |
| 83 | E | A | -1.1187 | |
| 84 | V | A | 1.4066 | |
| 85 | V | A | 1.6623 | |
| 86 | L | A | 0.1960 | |
| 87 | E | A | -1.7200 | |
| 88 | Q | A | -2.0231 | |
| 89 | V | A | -1.1687 | |
| 90 | T | A | -1.7026 | |
| 91 | E | A | -1.6939 | |
| 92 | N | A | -2.4354 | |
| 93 | F | A | 0.0000 | |
| 94 | N | A | -2.3579 | |
| 95 | M | A | 0.0000 | |
| 96 | W | A | 0.0000 | |
| 97 | K | A | -2.5754 | |
| 98 | N | A | -1.8766 | |
| 99 | D | A | -1.8814 | |
| 100 | M | A | 0.0000 | |
| 101 | V | A | 0.0000 | |
| 102 | D | A | -2.6433 | |
| 103 | Q | A | -1.7379 | |
| 104 | M | A | 0.0000 | |
| 105 | Q | A | -1.9806 | |
| 106 | E | A | -2.4966 | |
| 107 | D | A | -1.6099 | |
| 108 | V | A | 0.0000 | |
| 109 | I | A | -1.2856 | |
| 110 | S | A | -1.3510 | |
| 111 | I | A | 0.0000 | |
| 112 | W | A | 0.0000 | |
| 113 | D | A | -2.2659 | |
| 114 | Q | A | -1.4782 | |
| 115 | C | A | 0.0000 | |
| 116 | L | A | 0.0000 | |
| 117 | K | A | -1.3316 | |
| 118 | P | A | -0.3973 | |
| 119 | C | A | 0.1228 | |
| 120 | V | A | 0.4576 | |
| 121 | K | A | -0.0926 | |
| 122 | L | A | 0.0322 | |
| 123 | T | A | -0.6280 | |
| 124 | N | A | -1.0999 | |
| 198 | T | A | -0.7176 | |
| 199 | S | A | -0.4592 | |
| 200 | T | A | -0.1221 | |
| 201 | L | A | 0.3826 | |
| 202 | T | A | 0.1753 | |
| 203 | Q | A | -0.3207 | |
| 204 | A | A | -0.2634 | |
| 205 | C | A | -0.2956 | |
| 206 | P | A | -0.5876 | |
| 207 | K | A | -0.6854 | |
| 208 | V | A | -0.5408 | |
| 209 | T | A | -0.9256 | |
| 210 | F | A | 0.0000 | |
| 211 | D | A | -1.8383 | |
| 212 | P | A | -1.0574 | |
| 213 | I | A | -0.7890 | |
| 214 | P | A | -0.7568 | |
| 215 | I | A | 0.0000 | |
| 216 | H | A | -0.5121 | |
| 217 | Y | A | 0.0000 | |
| 218 | C | A | 0.0000 | |
| 219 | A | A | 0.0000 | |
| 220 | P | A | -0.0419 | |
| 221 | A | A | 0.0181 | |
| 222 | G | A | -0.1310 | |
| 223 | Y | A | -0.3776 | |
| 224 | A | A | -0.1868 | |
| 225 | I | A | 0.0000 | |
| 226 | L | A | 0.0000 | |
| 227 | K | A | -0.5193 | |
| 228 | C | A | 0.0000 | |
| 229 | N | A | -1.4531 | |
| 230 | N | A | -2.6146 | |
| 231 | K | A | -3.3716 | |
| 232 | T | A | -2.3098 | |
| 233 | F | A | 0.0000 | |
| 234 | N | A | -2.2047 | |
| 235 | G | A | 0.0000 | |
| 236 | K | A | -3.0225 | |
| 237 | G | A | -1.8719 | |
| 238 | P | A | -1.9907 | |
| 239 | C | A | 0.0000 | |
| 240 | N | A | -2.6792 | |
| 241 | N | A | -2.4748 | |
| 242 | V | A | 0.0000 | |
| 243 | S | A | 0.0000 | |
| 244 | T | A | 0.3599 | |
| 245 | V | A | -0.5670 | |
| 246 | Q | A | -1.2206 | |
| 247 | C | A | -0.8068 | |
| 248 | T | A | 0.0000 | |
| 249 | H | A | -0.6585 | |
| 250 | G | A | -0.7654 | |
| 251 | I | A | 0.0000 | |
| 252 | K | A | -1.0132 | |
| 253 | P | A | 0.0000 | |
| 254 | V | A | -0.3464 | |
| 255 | V | A | 0.0000 | |
| 256 | S | A | 0.0000 | |
| 257 | T | A | 0.0000 | |
| 258 | Q | A | 0.0000 | |
| 259 | L | A | 0.0000 | |
| 260 | L | A | 0.0000 | |
| 261 | L | A | 0.0000 | |
| 262 | N | A | -1.3295 | |
| 263 | G | A | -0.6848 | |
| 264 | S | A | -0.6350 | |
| 265 | L | A | -0.9390 | |
| 266 | A | A | 0.0000 | |
| 267 | E | A | -3.7156 | |
| 268 | E | A | -4.0622 | |
| 269 | E | A | -3.6115 | |
| 270 | I | A | 0.0000 | |
| 271 | V | A | 0.0000 | |
| 272 | I | A | 0.0000 | |
| 273 | R | A | 0.0000 | |
| 274 | S | A | -1.9110 | |
| 275 | K | A | -3.1397 | |
| 276 | N | A | -2.5450 | |
| 277 | L | A | -1.5088 | |
| 278 | R | A | -2.1303 | |
| 279 | A | A | -0.9915 | mutated: DA279A |
| 280 | S | A | 0.0400 | mutated: NA280S |
| 281 | V | A | 1.2514 | mutated: AA281V |
| 282 | N | A | -0.2683 | mutated: KA282N |
| 283 | T | A | -0.2892 | mutated: IA283T |
| 284 | I | A | 0.0000 | |
| 285 | I | A | 0.0000 | |
| 286 | V | A | 0.0000 | |
| 287 | Q | A | 0.0000 | |
| 288 | L | A | 0.0000 | |
| 289 | Q | A | -2.4557 | |
| 290 | K | A | -2.8652 | |
| 291 | S | A | -1.5893 | |
| 292 | V | A | 0.0000 | |
| 293 | E | A | -2.1611 | |
| 294 | I | A | 0.0000 | |
| 295 | V | A | -0.8950 | |
| 296 | C | A | 0.0000 | |
| 297 | T | A | 0.0000 | |
| 298 | R | A | 0.0000 | |
| 299 | P | A | -1.9818 | |
| 300 | N | A | -2.7570 | |
| 301 | N | A | -2.6850 | |
| 324 | G | A | -1.6486 | |
| 325 | D | A | -1.9314 | |
| 326 | I | A | -1.3269 | |
| 327 | R | A | -1.5467 | |
| 328 | Q | A | -1.5592 | |
| 329 | A | A | 0.0000 | |
| 330 | Y | A | -0.0473 | |
| 331 | C | A | 0.0000 | |
| 332 | Q | A | -0.4719 | |
| 333 | I | A | 0.0000 | |
| 334 | S | A | -1.3931 | |
| 335 | G | A | 0.0000 | |
| 336 | R | A | -3.1106 | |
| 337 | N | A | -2.6170 | |
| 338 | W | A | 0.0000 | |
| 339 | S | A | -2.5647 | |
| 340 | E | A | -3.3637 | |
| 341 | A | A | -2.4401 | |
| 342 | V | A | 0.0000 | |
| 343 | N | A | -2.8353 | |
| 344 | Q | A | -3.0036 | |
| 345 | V | A | 0.0000 | |
| 346 | K | A | -2.6524 | |
| 347 | K | A | -3.4500 | |
| 348 | K | A | -2.7290 | |
| 349 | L | A | 0.0000 | |
| 350 | K | A | -3.3006 | |
| 351 | E | A | -3.1247 | |
| 352 | H | A | -2.0574 | |
| 353 | F | A | -1.9377 | |
| 354 | P | A | -1.8933 | |
| 355 | H | A | -2.2308 | |
| 356 | K | A | -2.6622 | |
| 357 | N | A | -2.6494 | |
| 358 | I | A | 0.0000 | |
| 359 | S | A | 0.0000 | |
| 360 | F | A | 0.0000 | |
| 361 | Q | A | -1.3465 | |
| 362 | S | A | -1.4701 | |
| 363 | G | A | -1.8986 | mutated: SA363G |
| 364 | E | A | -3.0112 | mutated: SA364E |
| 365 | R | A | -3.1568 | mutated: GA365R |
| 366 | S | A | -2.2016 | mutated: GA366S |
| 367 | R | A | -1.9590 | mutated: DA367R |
| 368 | L | A | -0.5817 | |
| 369 | E | A | -0.4394 | |
| 370 | I | A | -1.0077 | |
| 371 | T | A | -1.3782 | |
| 372 | T | A | 0.0000 | |
| 373 | H | A | 0.0000 | |
| 374 | S | A | 0.0000 | |
| 375 | F | A | 0.0000 | |
| 376 | N | A | -0.3842 | |
| 377 | C | A | 0.0000 | |
| 378 | G | A | -0.6706 | |
| 379 | G | A | -0.4325 | |
| 380 | E | A | 0.0000 | |
| 381 | F | A | 0.0000 | |
| 382 | F | A | 0.0000 | |
| 383 | Y | A | 0.0000 | |
| 384 | C | A | 0.0000 | |
| 385 | N | A | -0.8632 | |
| 386 | T | A | 0.0000 | |
| 387 | S | A | -0.7931 | |
| 388 | G | A | -0.6831 | |
| 389 | L | A | 0.0000 | |
| 390 | F | A | 0.0000 | |
| 391 | Q | A | -1.6765 | |
| 392 | D | A | -2.2468 | |
| 393 | T | A | -1.1912 | |
| 394 | I | A | -1.2602 | |
| 395 | S | A | -1.2883 | |
| 396 | N | A | -2.4664 | |
| 412 | A | A | -0.9891 | |
| 413 | T | A | -0.5553 | |
| 414 | I | A | -0.0085 | |
| 415 | M | A | 0.7861 | |
| 416 | L | A | 0.0000 | |
| 417 | P | A | -0.4798 | |
| 418 | C | A | 0.0000 | |
| 419 | R | A | -1.7364 | |
| 420 | I | A | 0.0000 | |
| 421 | K | A | -0.5888 | |
| 422 | Q | A | 0.1582 | |
| 423 | I | A | 1.2495 | |
| 424 | I | A | 0.0000 | |
| 425 | N | A | -0.2870 | |
| 426 | M | A | -0.2093 | |
| 427 | W | A | -0.5301 | |
| 428 | Q | A | -1.1681 | |
| 429 | E | A | -1.1687 | |
| 430 | V | A | 0.4559 | |
| 431 | G | A | -0.5004 | |
| 432 | K | A | -0.5389 | |
| 433 | A | A | 0.0000 | |
| 434 | I | A | 0.7625 | |
| 435 | Y | A | 0.2374 | |
| 436 | A | A | 0.2077 | |
| 437 | P | A | -0.1399 | |
| 438 | P | A | -0.2588 | |
| 439 | I | A | -0.2126 | |
| 440 | K | A | -1.9781 | |
| 441 | G | A | -2.1290 | |
| 442 | Q | A | -2.3044 | |
| 443 | I | A | 0.0000 | |
| 444 | T | A | -0.6563 | |
| 445 | C | A | -1.0142 | |
| 446 | K | A | -2.1162 | |
| 447 | S | A | -1.8124 | |
| 448 | D | A | -2.3233 | |
| 449 | I | A | 0.0000 | |
| 450 | T | A | 0.0000 | |
| 451 | G | A | 0.0000 | |
| 452 | L | A | 0.0000 | |
| 453 | L | A | 0.0000 | |
| 454 | L | A | 0.0000 | |
| 455 | N | A | -0.0135 | mutated: LA455N |
| 456 | I | A | 0.0112 | mutated: RA456I |
| 457 | G | A | -0.3310 | mutated: DA457G |
| 458 | A | A | -0.3769 | mutated: GA458A |
| 459 | P | A | -1.1695 | mutated: GA459P |
| 460 | D | A | -2.3909 | |
| 461 | T | A | -2.0373 | |
| 462 | T | A | -1.9256 | |
| 463 | D | A | -2.9430 | |
| 464 | N | A | -3.0320 | |
| 465 | T | A | 0.0000 | |
| 466 | A | A | 0.0000 | mutated: EA466A |
| 467 | I | A | -0.2424 | |
| 468 | F | A | 0.0000 | |
| 469 | R | A | -1.0250 | |
| 470 | P | A | 0.0000 | |
| 471 | E | A | -1.0780 | mutated: SA471E |
| 472 | G | A | -0.5494 | |
| 473 | G | A | -0.8074 | |
| 474 | N | A | -0.8701 | mutated: DA474N |
| 475 | M | A | 0.0000 | |
| 476 | R | A | -1.7212 | |
| 477 | D | A | 0.0000 | |
| 478 | N | A | 0.0000 | |
| 479 | W | A | 0.0000 | |
| 480 | R | A | -0.9400 | |
| 481 | S | A | -0.2834 | |
| 482 | E | A | 0.0000 | |
| 483 | L | A | 0.0000 | |
| 484 | Y | A | 0.0000 | |
| 485 | K | A | -1.1558 | |
| 486 | Y | A | 0.0000 | |
| 487 | K | A | -1.2398 | |
| 488 | V | A | -0.8137 | |
| 489 | V | A | -1.0483 | |
| 490 | E | A | -1.2688 | |
| 491 | I | A | 0.3433 | |
| 492 | K | A | -1.2933 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine