Project name: LC-G200W [mutate: GL200W]

Chain sequence(s)	H: EVQLVESGGGLVQPGGSLRLSCAASGFAFSTYDMSWVRQAPGKGLEWVATISSGGSYTYYLDSVKGRFTISRDSSKNTLYLQMNSLRAEDTAVYYCAPTTVVPFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTSAA; L: DIQMTQSPSSLSASVGDRVTITCKASQNVRTVVAWYQQKPGKAPKTLIYLASNRHTGVPSRFSGSGSGTDFTLTISSLQPEDFATYFCLQHWSYPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GL200W
Energy difference between WT (input) and mutated protein (by FoldX)	18.0154 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.7178
• Maximal score value: 1.3012
• Average score: -0.7154
• Total score value: -316.2141

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	D	L	-2.4047
2	I	L	0.0000
3	Q	L	-2.0435
4	M	L	0.0000
5	T	L	-1.1056
6	Q	L	0.0000
7	S	L	-0.6023
8	P	L	-0.5589
9	S	L	-0.8399
10	S	L	-0.7074
11	L	L	-0.4819
12	S	L	-0.8719
13	A	L	0.0000
14	S	L	-0.5241
15	V	L	0.4439
16	G	L	-0.5836
17	D	L	-1.5498
18	R	L	-2.2395
19	V	L	0.0000
20	T	L	-0.5906
21	I	L	0.0000
22	T	L	-0.7518
23	C	L	0.0000
24	K	L	-2.1071
25	A	L	-1.9775
26	S	L	-2.0858
27	Q	L	-2.6921
28	N	L	-2.7267
29	V	L	0.0000
30	R	L	-2.5176
31	T	L	-1.2505
32	V	L	0.0000
33	V	L	0.0000
34	A	L	0.0000
35	W	L	0.0000
36	Y	L	0.0000
37	Q	L	0.0000
38	Q	L	-1.3609
39	K	L	-1.9152
40	P	L	-1.8054
41	G	L	-1.5559
42	K	L	-2.5372
43	A	L	-1.7566
44	P	L	0.0000
45	K	L	-1.7358
46	T	L	0.0000
47	L	L	0.0000
48	I	L	0.0000
49	Y	L	-0.0023
50	L	L	0.0482
51	A	L	0.0000
52	S	L	-0.9381
53	N	L	-1.3624
54	R	L	-2.2482
55	H	L	0.0000
56	T	L	-1.1816
57	G	L	-1.3246
58	V	L	0.0000
59	P	L	-0.7481
60	S	L	-0.8810
61	R	L	-1.0623
62	F	L	0.0000
63	S	L	-0.9274
64	G	L	0.0000
65	S	L	-0.6697
66	G	L	-1.2644
67	S	L	-1.7114
68	G	L	-2.2651
69	T	L	-2.3564
70	D	L	-2.6667
71	F	L	0.0000
72	T	L	-0.7465
73	L	L	0.0000
74	T	L	-0.6244
75	I	L	0.0000
76	S	L	-1.2979
77	S	L	-1.0831
78	L	L	0.0000
79	Q	L	-0.9910
80	P	L	-0.9774
81	E	L	-1.9898
82	D	L	0.0000
83	F	L	-0.6384
84	A	L	0.0000
85	T	L	-0.9062
86	Y	L	0.0000
87	F	L	0.0000
88	C	L	0.0000
89	L	L	0.0000
90	Q	L	0.0000
91	H	L	0.4095
92	W	L	0.1087
93	S	L	0.0428
94	Y	L	0.2526
95	P	L	-0.3376
96	L	L	0.0000
97	T	L	-0.7084
98	F	L	0.0000
99	G	L	0.0000
100	Q	L	-1.5518
101	G	L	0.0000
102	T	L	0.0000
103	K	L	-1.5679
104	V	L	0.0000
105	E	L	0.0000
106	I	L	-0.5711
107	K	L	-1.4991
108	R	L	-1.4297
109	T	L	-0.3703
110	V	L	-0.0382
111	A	L	-0.0174
112	A	L	0.0907
113	P	L	0.0000
114	S	L	-0.1725
115	V	L	0.0000
116	F	L	0.2573
117	I	L	0.1100
118	F	L	0.0000
119	P	L	-0.6988
120	P	L	0.0000
121	S	L	-1.3435
122	D	L	-2.6373
123	E	L	-2.1534
124	Q	L	0.0000
125	L	L	-1.8287
126	K	L	-2.5620
127	S	L	-1.5656
128	G	L	-1.3815
129	T	L	-1.1241
130	A	L	0.0000
131	S	L	0.0000
132	V	L	0.0000
133	V	L	0.0000
134	C	L	0.0000
135	L	L	0.0000
136	L	L	0.0000
137	N	L	0.0000
138	N	L	-1.3827
139	F	L	0.0000
140	Y	L	0.0000
141	P	L	-1.2836
142	R	L	-1.7878
143	E	L	-2.4652
144	A	L	-1.6099
145	K	L	-1.8289
146	V	L	-0.7033
147	Q	L	-0.2829
148	W	L	0.0000
149	K	L	-0.4105
150	V	L	0.0000
151	D	L	-1.7640
152	N	L	-1.4623
153	A	L	-0.2109
154	L	L	0.8277
155	Q	L	-0.1991
156	S	L	-0.6272
157	G	L	-1.2542
158	N	L	-1.5703
159	S	L	-1.3230
160	Q	L	-1.1815
161	E	L	-1.1093
162	S	L	-0.5279
163	V	L	-0.3381
164	T	L	-0.9213
165	E	L	-2.0572
166	Q	L	-1.9403
167	D	L	-2.3918
168	S	L	-2.0596
169	K	L	-2.8633
170	D	L	-3.0174
171	S	L	0.0000
172	T	L	0.0000
173	Y	L	0.0000
174	S	L	0.0000
175	L	L	0.0000
176	S	L	0.0000
177	S	L	0.0000
178	T	L	-0.5893
179	L	L	0.0000
180	T	L	-0.7159
181	L	L	-0.8347
182	S	L	-1.2121
183	K	L	-2.2016
184	A	L	-1.9190
185	D	L	-2.3961
186	Y	L	0.0000
187	E	L	-3.7178
188	K	L	-3.6145
189	H	L	-2.8936
190	K	L	-3.1515
191	V	L	-1.4821
192	Y	L	0.0000
193	A	L	0.0000
194	C	L	0.0000
195	E	L	-0.7692
196	V	L	0.0000
197	T	L	-0.8810
198	H	L	0.0000
199	Q	L	-1.1056
200	W	L	0.4762	mutated: GL200W
201	L	L	0.1618
202	S	L	-0.2025
203	S	L	-0.3059
204	P	L	-0.5950
205	V	L	-0.0475
206	T	L	-0.4824
207	K	L	-0.7675
208	S	L	-0.7346
209	F	L	0.0000
210	N	L	-2.0075
211	R	L	-2.8905
212	G	L	-1.9714
213	E	L	-1.8212
214	C	L	0.0000
1	E	H	-1.9562
2	V	H	-0.6307
3	Q	H	-1.2102
4	L	H	0.0000
5	V	H	1.2444
6	E	H	0.0000
7	S	H	-0.2156
8	G	H	-0.5477
9	G	H	-0.4062
10	G	H	-0.0532
11	L	H	0.2281
12	V	H	-0.2130
13	Q	H	-1.0693
14	P	H	-1.5002
15	G	H	-1.3446
16	G	H	-1.1320
17	S	H	-1.4511
18	L	H	-1.2710
19	R	H	-2.1016
20	L	H	0.0000
21	S	H	-0.3025
22	C	H	0.0000
23	A	H	0.0109
24	A	H	0.0000
25	S	H	-0.3568
26	G	H	-0.7201
27	F	H	0.0000
28	A	H	-0.1996
29	F	H	0.0000
30	S	H	-0.8209
31	T	H	-0.3237
32	Y	H	0.0158
33	D	H	-0.2797
34	M	H	0.0000
35	S	H	0.0000
36	W	H	0.0000
37	V	H	0.0000
38	R	H	-0.6919
39	Q	H	-0.7618
40	A	H	-1.0392
41	P	H	-0.8726
42	G	H	-1.4639
43	K	H	-2.3443
44	G	H	-1.5691
45	L	H	0.0000
46	E	H	-0.8832
47	W	H	0.0000
48	V	H	0.0000
49	A	H	0.0000
50	T	H	0.0000
51	I	H	0.0000
52	S	H	-0.0864
53	S	H	0.0000
54	G	H	-0.6669
55	G	H	-0.3927
56	S	H	-0.0942
57	Y	H	1.0160
58	T	H	0.7126
59	Y	H	0.4138
60	Y	H	-0.6952
61	L	H	-1.3435
62	D	H	-2.6513
63	S	H	-1.6454
64	V	H	0.0000
65	K	H	-2.7617
66	G	H	-2.0559
67	R	H	-2.3985
68	F	H	0.0000
69	T	H	-1.0548
70	I	H	0.0000
71	S	H	-0.2472
72	R	H	-1.1231
73	D	H	-1.3752
74	S	H	-1.3306
75	S	H	-1.4015
76	K	H	-2.2322
77	N	H	-1.5807
78	T	H	-0.8878
79	L	H	0.0000
80	Y	H	-0.4157
81	L	H	0.0000
82	Q	H	-1.3796
83	M	H	0.0000
84	N	H	-2.0746
85	S	H	-1.5588
86	L	H	0.0000
87	R	H	-2.3768
88	A	H	-1.6602
89	E	H	-2.2215
90	D	H	0.0000
91	T	H	-0.4672
92	A	H	0.0000
93	V	H	0.5461
94	Y	H	0.0000
95	Y	H	0.0000
96	C	H	0.0000
97	A	H	0.0000
98	P	H	0.0000
99	T	H	0.0000
100	T	H	0.7004
101	V	H	1.3012
102	V	H	1.1914
103	P	H	0.1036
104	F	H	0.5368
105	A	H	0.0150
106	Y	H	0.0000
107	W	H	0.0000
108	G	H	0.0000
109	Q	H	-1.0364
110	G	H	-0.3854
111	T	H	0.0000
112	L	H	1.2049
113	V	H	0.0000
114	T	H	-0.1669
115	V	H	0.0000
116	S	H	-0.8487
117	S	H	-0.6853
118	A	H	-0.4489
119	S	H	-0.5733
120	T	H	-0.8421
121	K	H	-1.3639
122	G	H	-1.4245
123	P	H	0.0000
124	S	H	-0.4824
125	V	H	0.0000
126	F	H	0.0000
127	P	H	-1.0845
128	L	H	0.0000
129	A	H	-0.9485
130	P	H	0.0000
131	S	H	-1.2782
132	S	H	-1.5989
133	K	H	-1.9826
134	S	H	-1.3723
135	T	H	-0.9396
136	S	H	-0.8007
137	G	H	-0.7736
138	G	H	-0.5906
139	T	H	-0.4445
140	A	H	-0.3591
141	A	H	0.0000
142	L	H	0.0000
143	G	H	0.0000
144	C	H	0.0000
145	L	H	0.0000
146	V	H	0.0000
147	K	H	-0.3900
148	D	H	-0.6632
149	Y	H	0.0000
150	F	H	0.0000
151	P	H	-1.0078
152	E	H	-1.2531
153	P	H	-1.3239
154	V	H	0.0000
155	T	H	-0.9596
156	V	H	-0.4674
157	S	H	-0.4053
158	W	H	0.0000
159	N	H	-0.7678
160	S	H	-0.6396
161	G	H	-0.6351
162	A	H	-0.3127
163	L	H	-0.1030
164	T	H	-0.2385
165	S	H	-0.2207
166	G	H	-0.3334
167	V	H	0.0823
168	H	H	-0.2939
169	T	H	0.0149
170	F	H	0.0000
171	P	H	-0.3973
172	A	H	-0.0907
173	V	H	0.2078
174	L	H	0.7302
175	Q	H	0.1232
176	S	H	-0.1066
177	S	H	-0.2300
178	G	H	-0.0207
179	L	H	-0.2037
180	Y	H	0.0000
181	S	H	0.0000
182	L	H	0.0000
183	S	H	0.0000
184	S	H	0.0000
185	V	H	0.0000
186	V	H	0.0000
187	T	H	-0.1082
188	V	H	0.0000
189	P	H	-0.6286
190	S	H	0.0000
191	S	H	-0.7818
192	S	H	-0.7774
193	L	H	0.0000
194	G	H	-1.2434
195	T	H	-1.1115
196	Q	H	-1.5186
197	T	H	-1.2165
198	Y	H	0.0000
199	I	H	-1.1667
200	C	H	0.0000
201	N	H	-1.2859
202	V	H	0.0000
203	N	H	-2.2736
204	H	H	0.0000
205	K	H	-2.9654
206	P	H	-1.8080
207	S	H	-1.8799
208	N	H	-2.6231
209	T	H	-2.1637
210	K	H	-2.6518
211	V	H	-1.3936
212	D	H	-1.8896
213	K	H	-1.6430
214	K	H	-2.3533
215	V	H	0.0000
216	E	H	-2.5725
217	P	H	-1.5474
218	K	H	-1.3844
219	S	H	-1.3162
220	C	H	-1.8849
221	D	H	-2.8924
222	K	H	-2.7991
223	T	H	-2.1472
224	H	H	-1.8362
225	T	H	-1.3599
226	S	H	-0.6577
227	A	H	-1.1415
228	A	H	-1.2273