Project name: insulin [mutate: CC7A]

Chain sequence(s)	A: GIVEQCCTSICSLYQLENYCN; B: FVNQHLCGSHLVVEALYLVCGERRGFFYTPKA
Selected Chain(s)	A,B
Distance of aggregation	10 Å
FoldX usage	Yes
Dynamic mode	No
Automated mutations	No
Mutated residues	CC7A
Energy difference between WT (input) and mutated protein (by FoldX)	-0.0593577 kcal/mol

Simulation log

[INFO]       Logger:   Verbosity set to: 2 - [INFO]                                                (00:00:00)
[WARNING]    runJob:   Working directory already exists (possibly overwriting previous results -ow 
                       to prevent this behavior)                                                   (00:00:00)
[INFO]       runJob:   Starting aggrescan3d job on: input.pdb with all chain(s) selected           (00:00:00)
[INFO]       runJob:   Creating pdb object from: input.pdb                                         (00:00:00)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:00)
[INFO]       FoldX:    Building mutant model                                                       (00:00:16)
[INFO]       FoldX:    Starting FoldX energy minimalization                                        (00:00:16)
[INFO]       Analysis: Starting Aggrescan3D on folded.pdb                                          (00:00:25)
[INFO]       Main:     Simulation completed successfully.                                          (00:00:26)

• Minimal score value: -2.4178
• Maximal score value: 1.5308
• Average score: -0.414
• Total score value: -21.1126

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	G	A	-1.3962
2	I	A	0.0000
3	V	A	-1.2637
4	E	A	-1.9368
5	Q	A	-1.4234
6	C	A	0.0000
7	C	A	-0.7770	mutated: CC7A
8	T	A	-0.6218
9	S	A	-0.3610
10	I	A	0.1208
11	C	A	0.0000
12	S	A	0.4535
13	L	A	1.1358
14	Y	A	1.0767
15	Q	A	-0.1636
16	L	A	0.0000
17	E	A	-1.1070
18	N	A	-1.3552
19	Y	A	-0.5266
20	C	A	-1.0763
21	N	A	-1.8180
1	F	B	1.2502
2	V	B	0.5758
3	N	B	-0.6775
4	Q	B	-0.9723
5	H	B	-0.9635
6	L	B	0.0000
7	C	B	-0.8281
8	G	B	-0.6429
9	S	B	-1.0965
10	H	B	-1.3049
11	L	B	0.0000
12	V	B	0.0959
13	E	B	-0.9569
14	A	B	0.0000
15	L	B	0.0000
16	Y	B	1.0305
17	L	B	1.2745
18	V	B	0.4971
19	C	B	0.0000
20	G	B	-1.0482
21	E	B	-2.4178
22	R	B	-2.3712
23	G	B	-0.7523
24	F	B	0.2440
25	F	B	1.5308
26	Y	B	1.1737
27	T	B	0.0038
28	P	B	-0.9523
29	K	B	-1.7191
30	A	B	-1.0456