Project name: SH3_L86C

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA86C
Energy difference between WT (input) and mutated protein (by FoldX)	1.40484 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.6671
• Average score: -1.0054
• Total score value: -57.3098

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.2200
86	C	A	0.2102	mutated: LA86C
87	F	A	0.5863
88	V	A	0.2477
89	A	A	0.0000
90	L	A	-0.1205
91	Y	A	-0.5354
92	D	A	-2.5201
93	Y	A	-1.9321
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0436
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2061
103	F	A	0.0000
104	H	A	-2.6808
105	K	A	-2.3071
106	G	A	-1.2465
107	E	A	-1.2266
108	K	A	-0.8709
109	F	A	0.0000
110	Q	A	-1.1482
111	I	A	-0.3638
112	L	A	0.0886
113	N	A	-0.9142
114	S	A	-1.1623
115	S	A	-1.5884
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0903
121	E	A	-1.1936
122	A	A	0.0000
123	R	A	-1.8572
124	S	A	0.0000
125	L	A	0.0168
126	T	A	-0.3896
127	T	A	-0.7841
128	G	A	-1.3632
129	E	A	-2.2067
130	T	A	-1.7157
131	G	A	-1.4836
132	Y	A	-0.8544
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9195
136	N	A	-1.1511
137	Y	A	-0.1379
138	V	A	0.0000
139	A	A	0.3475
140	P	A	0.5805
141	V	A	1.6671