Project name: SH3_D92G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	DA92G
Energy difference between WT (input) and mutated protein (by FoldX)	1.59016 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1039
• Maximal score value: 1.7964
• Average score: -0.8672
• Total score value: -49.4291

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9248
88	V	A	0.4335
89	A	A	0.0000
90	L	A	0.1221
91	Y	A	0.0731
92	G	A	-1.2333	mutated: DA92G
93	Y	A	-1.2777
94	E	A	-2.3448
95	A	A	-2.4919
96	R	A	-2.9855
97	T	A	-2.6619
98	E	A	-3.1039
99	D	A	-3.0444
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-1.9736
103	F	A	0.0000
104	H	A	-2.1277
105	K	A	-1.8858
106	G	A	-1.2527
107	E	A	-1.1209
108	K	A	-0.6393
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0606
126	T	A	-0.4713
127	T	A	-0.8199
128	G	A	-1.3572
129	E	A	-2.2413
130	T	A	-1.6937
131	G	A	-1.5006
132	Y	A	-0.8686
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	0.0772
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964