Project name: SH3_F87M

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FA87M
Energy difference between WT (input) and mutated protein (by FoldX)	1.34105 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7175
• Average score: -0.9861
• Total score value: -56.2096

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4686
86	L	A	0.6891
87	M	A	0.0000	mutated: FA87M
88	V	A	0.3184
89	A	A	0.0000
90	L	A	-0.1783
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4856
107	E	A	-1.3428
108	K	A	-0.7107
109	F	A	0.0000
110	Q	A	-0.5440
111	I	A	-0.0572
112	L	A	0.1671
113	N	A	-0.8679
114	S	A	-1.1598
115	S	A	-1.5910
116	E	A	-2.5540
117	G	A	-2.1277
118	D	A	-2.4361
119	W	A	-1.0881
120	W	A	-1.0181
121	E	A	-1.1286
122	A	A	0.0000
123	R	A	-1.7033
124	S	A	0.0000
125	L	A	0.0353
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6883
131	G	A	-1.4906
132	Y	A	-0.8534
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9358
136	N	A	-1.1468
137	Y	A	-0.1505
138	V	A	0.0000
139	A	A	0.3376
140	P	A	0.6759
141	V	A	1.7175