Project name: rbd10 [mutate: LC455R, IC472P, AC475N, FC456A, FC490K, SC494R]

Chain sequence(s)	C: TNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGP
Distance of aggregation	5 Å
Dynamic mode	No
Mutated residues	LC455R, IC472P, AC475N, FC456A, FC490K, SC494R
Energy difference between WT (input) and mutated protein (by FoldX)	4.92146 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.1282
• Maximal score value: 1.738
• Average score: -0.249
• Total score value: -48.5569

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
333	T	C	-0.3035
334	N	C	-0.9920
335	L	C	1.3270
336	C	C	0.3527
337	P	C	-0.0218
338	F	C	0.1387
339	G	C	-0.6386
340	E	C	-1.8831
341	V	C	0.0000
342	F	C	0.0000
343	N	C	-1.2701
344	A	C	-0.2307
345	T	C	-0.4052
346	R	C	-1.8570
347	F	C	0.0000
348	A	C	0.0045
349	S	C	-0.0232
350	V	C	0.0000
351	Y	C	0.1562
352	A	C	0.0371
353	W	C	0.0000
354	N	C	-1.4285
355	R	C	-1.2266
356	K	C	-1.3025
357	R	C	-1.9796
358	I	C	0.0000
359	S	C	-0.3248
360	N	C	-1.2898
361	C	C	0.0000
362	V	C	0.7214
363	A	C	-0.1302
364	D	C	-1.4664
365	Y	C	0.0000
366	S	C	0.1350
367	V	C	1.7380
368	L	C	0.0000
369	Y	C	1.0797
370	N	C	-1.0430
371	S	C	-0.2958
372	A	C	0.0061
373	S	C	-0.1519
374	F	C	0.2138
375	S	C	-0.1517
376	T	C	0.0480
377	F	C	0.3447
378	K	C	-1.1161
379	C	C	0.0131
380	Y	C	0.4301
381	G	C	-0.3033
382	V	C	0.2238
383	S	C	-0.0363
384	P	C	-0.0605
385	T	C	-0.3870
386	K	C	-1.7129
387	L	C	0.0000
388	N	C	-0.8236
389	D	C	-1.7587
390	L	C	0.3638
391	C	C	0.4098
392	F	C	0.2746
393	T	C	0.0000
394	N	C	-0.1758
395	V	C	0.0000
396	Y	C	-0.0700
397	A	C	0.0000
398	D	C	0.0000
399	S	C	0.0000
400	F	C	0.0000
401	V	C	0.0000
402	I	C	0.0000
403	R	C	-0.2825
404	G	C	-0.4335
405	D	C	-1.5381
406	E	C	0.0000
407	V	C	-0.1275
408	R	C	-1.8302
409	Q	C	-0.4717
410	I	C	0.0000
411	A	C	-0.0030
412	P	C	-0.3343
413	G	C	-0.5572
414	Q	C	-0.5419
415	T	C	-0.1709
416	G	C	-0.2837
417	K	C	-0.9377
418	I	C	0.0000
419	A	C	0.0000
420	D	C	-0.3248
421	Y	C	0.1735
422	N	C	0.0000
423	Y	C	0.0000
424	K	C	-0.5380
425	L	C	0.0000
426	P	C	-0.3687
427	D	C	-2.1282
428	D	C	-2.1159
429	F	C	0.0000
430	T	C	-0.1233
431	G	C	0.0000
432	C	C	0.0000
433	V	C	0.0000
434	I	C	0.0000
435	A	C	0.0000
436	W	C	0.0631
437	N	C	-0.4061
438	S	C	0.0000
439	N	C	-0.4797
440	N	C	-1.0368
441	L	C	1.3163
442	D	C	0.0000
443	S	C	-0.3503
444	K	C	-1.3995
445	V	C	1.3726
446	G	C	-0.1478
447	G	C	-0.4174
448	N	C	-0.2083
449	Y	C	0.4779
450	N	C	-1.1390
451	Y	C	0.0000
452	L	C	0.1600
453	Y	C	0.2106
454	R	C	0.0000
455	R	C	-1.0671	mutated: LC455R
456	A	C	0.0000	mutated: FC456A
457	R	C	-0.7250
458	K	C	-1.8147
459	S	C	-0.7586
460	N	C	-1.3370
461	L	C	0.0000
462	K	C	-1.7192
463	P	C	-0.3622
464	F	C	0.5104
465	E	C	-0.5867
466	R	C	-1.4662
467	D	C	0.0000
468	I	C	1.2041
469	S	C	0.0868
470	T	C	-0.4263
471	E	C	-1.8452
472	P	C	-0.3558	mutated: IC472P
473	Y	C	0.1329
474	Q	C	-0.7991
475	N	C	-1.5120	mutated: AC475N
476	G	C	-0.5385
477	S	C	-0.2559
478	T	C	-0.1565
479	P	C	-0.2532
480	C	C	-0.2014
481	N	C	-1.3451
482	G	C	-0.3794
483	V	C	1.3506
484	E	C	-1.5536
485	G	C	-0.3609
486	F	C	1.6964
487	N	C	-0.7257
488	C	C	-0.2779
489	Y	C	0.2548
490	K	C	-1.2468	mutated: FC490K
491	P	C	0.0000
492	L	C	0.1128
493	Q	C	-0.7291
494	R	C	-1.8713	mutated: SC494R
495	Y	C	0.0000
496	G	C	-0.0641
497	F	C	0.0000
498	Q	C	-0.4885
499	P	C	-0.2464
500	T	C	-0.1466
501	N	C	-0.3626
502	G	C	-0.1854
503	V	C	1.6570
504	G	C	0.2428
505	Y	C	0.4031
506	Q	C	-0.0526
507	P	C	0.0000
508	Y	C	0.0839
509	R	C	0.0000
510	V	C	0.0000
511	V	C	0.0000
512	V	C	0.0000
513	L	C	0.0000
514	S	C	-0.0208
515	F	C	0.0000
516	E	C	-0.4525
517	L	C	1.6982
518	L	C	1.6525
519	H	C	-0.7004
520	A	C	-0.2040
521	P	C	-0.2455
522	A	C	0.0009
523	T	C	-0.0153
524	V	C	0.0000
525	C	C	0.3766
526	G	C	-0.1338
527	P	C	-0.2677