Project name: SH3_F87H

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FA87H
Energy difference between WT (input) and mutated protein (by FoldX)	3.30878 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7178
• Average score: -0.99
• Total score value: -56.4281

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4616
86	L	A	0.6801
87	H	A	0.0000	mutated: FA87H
88	V	A	0.3187
89	A	A	0.0000
90	L	A	-0.1781
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4855
107	E	A	-1.3428
108	K	A	-0.7151
109	F	A	0.0000
110	Q	A	-0.5653
111	I	A	-0.1009
112	L	A	0.1450
113	N	A	-0.8827
114	S	A	-1.1726
115	S	A	-1.5927
116	E	A	-2.5558
117	G	A	-2.1298
118	D	A	-2.4389
119	W	A	-1.0929
120	W	A	-1.0368
121	E	A	-1.1442
122	A	A	0.0000
123	R	A	-1.7144
124	S	A	0.0000
125	L	A	0.0353
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6932
131	G	A	-1.4963
132	Y	A	-0.8611
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9434
136	N	A	-1.1480
137	Y	A	-0.1505
138	V	A	0.0000
139	A	A	0.3385
140	P	A	0.6764
141	V	A	1.7178