Project name: SH3_R96C

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	RA96C
Energy difference between WT (input) and mutated protein (by FoldX)	1.46433 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.7207
• Maximal score value: 1.7964
• Average score: -0.855
• Total score value: -48.7368

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9235
88	V	A	0.4306
89	A	A	0.0000
90	L	A	-0.1474
91	Y	A	-0.5666
92	D	A	-2.5441
93	Y	A	-1.6222
94	E	A	-2.0341
95	A	A	-1.4003
96	C	A	-0.4466	mutated: RA96C
97	T	A	-1.4646
98	E	A	-2.6209
99	D	A	-2.6946
100	D	A	-1.5933
101	L	A	0.0000
102	S	A	-1.8370
103	F	A	0.0000
104	H	A	-2.7207
105	K	A	-2.4009
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5090
111	I	A	-0.0566
112	L	A	0.1431
113	N	A	-0.8648
114	S	A	-1.1452
115	S	A	-1.5760
116	E	A	-2.5297
117	G	A	-2.0891
118	D	A	-2.3658
119	W	A	-0.9645
120	W	A	-0.9941
121	E	A	-1.1393
122	A	A	0.0000
123	R	A	-1.7154
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.7176
131	G	A	-1.5396
132	Y	A	-0.8915
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8677
136	N	A	-1.1257
137	Y	A	-0.1012
138	V	A	0.0000
139	A	A	0.4159
140	P	A	0.7757
141	V	A	1.7964