Project name: 3D6AB

Chain sequence(s)	A: DAEFRH; H: VQLLESGGGLVQPGGSLRLSCAASGFTFSNYGMSWVRQAPGKGLEWVASIRSGGGRTYYSDNVKGRFTISRDNAKNSLYLQMNSLRAEDTALYYCVRYDHYSGSSDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPQPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK; L: YVVMTQSPLSLPVTPGEPASISCKSSQSLLDSDGKTYLNWLLQKPGQSPQRLIYLVSKLDSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCWQGTHFPRTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
Distance of aggregation	5 Å
Dynamic mode	No

• Minimal score value: -2.221
• Maximal score value: 1.7728
• Average score: -0.2949
• Total score value: -130.9552

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
2	V	H	1.4251
3	Q	H	-0.5410
4	L	H	0.0000
5	L	H	1.0309
6	E	H	0.0000
7	S	H	-0.2820
8	G	H	-0.4455
9	G	H	-0.2325
10	G	H	-0.0413
11	L	H	0.2793
12	V	H	0.0000
13	Q	H	-1.2273
14	P	H	-0.4507
15	G	H	-0.4418
16	G	H	0.0000
17	S	H	-0.2303
18	L	H	-0.1267
19	R	H	-1.8285
20	L	H	0.0000
21	S	H	-0.0321
22	C	H	0.0000
23	A	H	0.0410
24	A	H	0.0000
25	S	H	-0.1716
26	G	H	-0.1989
27	F	H	0.1525
28	T	H	-0.0244
29	F	H	0.0000
30	S	H	-0.3521
31	N	H	-1.2692
32	Y	H	-0.0929
33	G	H	0.0000
34	M	H	0.0000
35	S	H	0.0000
36	W	H	0.0000
37	V	H	0.0000
38	R	H	0.0000
39	Q	H	-0.1331
40	A	H	-0.0605
41	P	H	-0.3386
42	G	H	-0.8228
43	K	H	-1.8168
44	G	H	-0.4873
45	L	H	0.0000
46	E	H	-0.5724
47	W	H	0.0000
48	V	H	0.0000
49	A	H	0.0000
50	S	H	0.0000
51	I	H	0.0000
52	R	H	-0.3409
52A	S	H	-0.2031
53	G	H	-0.3793
54	G	H	-0.6038
55	G	H	-0.8899
56	R	H	-1.9343
57	T	H	-0.3224
58	Y	H	0.2552
59	Y	H	0.2551
60	S	H	-0.3122
61	D	H	-2.0251
62	N	H	-1.5986
63	V	H	0.0000
64	K	H	-1.7874
65	G	H	-0.8403
66	R	H	-0.3842
67	F	H	0.0000
68	T	H	-0.0549
69	I	H	0.0000
70	S	H	-0.1563
71	R	H	-0.3544
72	D	H	-0.5717
73	N	H	-0.7552
74	A	H	-0.3692
75	K	H	-1.7795
76	N	H	-0.8746
77	S	H	0.0000
78	L	H	0.0000
79	Y	H	0.2561
80	L	H	0.0000
81	Q	H	-0.4597
82	M	H	0.0000
82A	N	H	-0.6859
82B	S	H	-0.3054
82C	L	H	0.0000
83	R	H	-1.1192
84	A	H	-0.4822
85	E	H	-1.8092
86	D	H	0.0000
87	T	H	-0.0172
88	A	H	0.0000
89	L	H	0.3579
90	Y	H	0.0000
91	Y	H	0.0000
92	C	H	0.0000
93	V	H	0.0000
94	R	H	0.0000
95	Y	H	0.0000
96	D	H	0.0000
97	H	H	-0.3680
98	Y	H	1.2069
99	S	H	0.0000
100	G	H	-0.1111
100A	S	H	0.0000
100B	S	H	0.0000
101	D	H	0.0000
102	Y	H	0.7187
103	W	H	0.0000
104	G	H	0.0000
105	Q	H	-1.1983
106	G	H	0.0000
107	T	H	0.0284
108	L	H	0.2237
109	V	H	0.0000
110	T	H	0.0242
111	V	H	0.0000
112	S	H	-0.0815
113	S	H	-0.2138
114	A	H	-0.0412
115	S	H	-0.2175
116	T	H	-0.2697
117	K	H	-0.9417
118	G	H	-0.3081
119	P	H	-0.0696
120	S	H	-0.1040
121	V	H	0.0000
122	F	H	0.0000
123	P	H	-0.0338
124	L	H	0.0000
125	A	H	0.0050
126	P	H	-0.0324
127	S	H	-0.0606
128	S	H	-0.5593
129	K	H	-1.7710
130	S	H	-0.6885
131	T	H	-0.1209
132	S	H	-0.3062
133	G	H	-0.5510
134	G	H	-0.3391
135	T	H	-0.1241
136	A	H	0.0009
137	A	H	0.0000
138	L	H	0.0000
139	G	H	0.0000
140	C	H	0.0000
141	L	H	0.0000
142	V	H	0.0000
143	K	H	-0.2391
144	D	H	-0.3932
145	Y	H	0.0000
146	F	H	0.0000
147	P	H	0.0000
148	Q	H	-0.3676
149	P	H	-0.1921
150	V	H	0.1614
151	T	H	0.0265
152	V	H	0.1874
153	S	H	-0.0463
154	W	H	0.0000
155	N	H	-0.2222
156	S	H	-0.3401
157	G	H	-0.4902
158	A	H	0.0164
159	L	H	0.2300
160	T	H	-0.0668
161	S	H	-0.2715
162	G	H	-0.2132
163	V	H	0.3374
164	H	H	-0.0556
165	T	H	-0.0548
166	F	H	0.0000
167	P	H	-0.1683
168	A	H	0.0418
169	V	H	0.5309
170	L	H	1.1583
171	Q	H	-0.0032
172	S	H	-0.2826
173	S	H	-0.2867
174	G	H	-0.1849
175	L	H	0.2457
176	Y	H	0.4260
177	S	H	0.0000
178	L	H	0.0000
179	S	H	0.0000
180	S	H	0.0000
181	V	H	0.0000
182	V	H	0.0000
183	T	H	-0.0183
184	V	H	0.0000
185	P	H	-0.2543
186	S	H	-0.1815
187	S	H	-0.2624
188	S	H	-0.0167
189	L	H	0.3016
190	G	H	-0.4058
191	T	H	-0.3751
192	Q	H	-1.1944
193	T	H	-0.2581
194	Y	H	0.0000
195	I	H	0.0964
196	C	H	0.0000
197	N	H	-0.3880
198	V	H	0.0000
199	N	H	-0.4111
200	H	H	0.0000
201	K	H	-1.8614
202	P	H	-0.3883
203	S	H	-0.3024
204	N	H	-1.4848
205	T	H	-0.5635
206	K	H	-1.6254
207	V	H	-0.1047
208	D	H	-1.7561
209	K	H	-0.8976
210	K	H	-1.6824
211	V	H	0.0000
212	E	H	-1.8498
213	P	H	-0.8037
214	K	H	-1.7303
1	Y	L	1.3153
2	V	L	0.0000
3	V	L	1.7728
4	M	L	0.0000
5	T	L	-0.0387
6	Q	L	0.0000
7	S	L	-0.1828
8	P	L	0.0740
9	L	L	1.2152
10	S	L	-0.1209
11	L	L	0.1183
12	P	L	-0.1826
13	V	L	0.0000
14	T	L	-0.2182
15	P	L	-0.1405
16	G	L	-0.6706
17	E	L	-2.0951
18	P	L	-0.8379
19	A	L	0.0000
20	S	L	-0.3175
21	I	L	0.0000
22	S	L	-0.0632
23	C	L	0.0000
24	K	L	-1.2631
25	S	L	0.0000
26	S	L	-0.3264
27	Q	L	-0.6599
27A	S	L	-0.2014
27B	L	L	0.0000
27C	L	L	1.5114
27D	D	L	0.0548
27E	S	L	-0.5740
28	D	L	-1.9137
29	G	L	-1.0053
30	K	L	-1.2260
31	T	L	-0.0183
32	Y	L	0.0000
33	L	L	0.0000
34	N	L	0.0000
35	W	L	0.0000
36	L	L	0.0000
37	L	L	0.0000
38	Q	L	-0.2097
39	K	L	-0.4001
40	P	L	-0.2089
41	G	L	-0.6949
42	Q	L	-1.2931
43	S	L	-0.2744
44	P	L	0.0000
45	Q	L	-0.9882
46	R	L	0.0000
47	L	L	0.0000
48	I	L	0.0000
49	Y	L	0.2083
50	L	L	0.2232
51	V	L	0.0000
52	S	L	-0.3887
53	K	L	-1.1088
54	L	L	0.1890
55	D	L	-0.2806
56	S	L	-0.3614
57	G	L	-0.5066
58	V	L	0.0000
59	P	L	-0.4360
60	D	L	-1.8743
61	R	L	-0.6868
62	F	L	0.0000
63	S	L	-0.1867
64	G	L	-0.1430
65	S	L	-0.2609
66	G	L	-0.2927
67	S	L	-0.2649
68	G	L	-0.1201
69	T	L	-0.2994
70	D	L	-1.5286
71	F	L	0.0000
72	T	L	-0.0260
73	L	L	0.0000
74	K	L	-1.7124
75	I	L	0.0000
76	S	L	-0.5917
77	R	L	-2.1351
78	V	L	0.0000
79	E	L	-1.2887
80	A	L	-0.5643
81	E	L	-1.8226
82	D	L	0.0000
83	V	L	0.0000
84	G	L	0.0000
85	V	L	-0.0101
86	Y	L	0.0000
87	Y	L	0.0000
88	C	L	0.0000
89	W	L	0.0000
90	Q	L	0.0000
91	G	L	0.0000
92	T	L	0.0000
93	H	L	-0.0236
94	F	L	0.9294
95	P	L	0.2077
96	R	L	0.0000
97	T	L	0.0269
98	F	L	0.2097
99	G	L	0.0000
100	Q	L	-1.0143
101	G	L	0.0000
102	T	L	0.0000
103	K	L	-1.6900
104	V	L	0.0000
105	E	L	-0.4600
106	I	L	0.0000
107	K	L	-1.2521
108	R	L	-0.6475
109	T	L	0.0326
110	V	L	0.9623
111	A	L	0.1965
112	A	L	0.0024
113	P	L	0.0000
114	S	L	-0.1878
115	V	L	0.0000
116	F	L	0.2681
117	I	L	0.0000
118	F	L	0.0000
119	P	L	-0.0264
120	P	L	0.0000
121	S	L	0.0000
122	D	L	-1.9292
123	E	L	-1.3501
124	Q	L	0.0000
125	L	L	-0.2270
126	K	L	-1.6589
127	S	L	-0.6087
128	G	L	-0.5088
129	T	L	-0.3049
130	A	L	0.0000
131	S	L	0.0000
132	V	L	0.0000
133	V	L	0.0000
134	C	L	0.0000
135	L	L	0.0000
136	L	L	0.0000
137	N	L	0.0000
138	N	L	-0.5445
139	F	L	0.0000
140	Y	L	0.0000
141	P	L	-0.3730
142	R	L	-2.1848
143	E	L	-2.1573
144	A	L	-0.5247
145	K	L	-1.0756
146	V	L	-0.0815
147	Q	L	-0.4169
148	W	L	0.0000
149	K	L	-0.2682
150	V	L	0.0000
151	D	L	-0.6057
152	N	L	-1.3442
153	A	L	-0.0063
154	L	L	1.2006
155	Q	L	-0.1151
156	S	L	-0.3567
157	G	L	-0.7398
158	N	L	-1.3757
159	S	L	-0.4160
160	Q	L	-0.8406
161	E	L	-1.9144
162	S	L	-0.3091
163	V	L	0.2881
164	T	L	-0.2945
165	E	L	-1.8214
166	Q	L	0.0000
167	D	L	-0.2504
168	S	L	-0.6014
169	K	L	-1.8589
170	D	L	-1.1421
171	S	L	0.0000
172	T	L	0.0000
173	Y	L	0.0000
174	S	L	0.0000
175	L	L	0.0000
176	S	L	0.0000
177	S	L	0.0000
178	T	L	-0.0104
179	L	L	0.0000
180	T	L	0.0109
181	L	L	0.1478
182	S	L	-0.4729
183	K	L	-1.7141
184	A	L	-0.4549
185	D	L	-1.2749
186	Y	L	0.0000
187	E	L	-2.2210
188	K	L	-2.1889
189	H	L	-0.9581
190	K	L	-1.6922
191	V	L	0.0178
192	Y	L	0.0000
193	A	L	0.0000
194	C	L	0.0000
195	E	L	-0.2586
196	V	L	0.0000
197	T	L	-0.1568
198	H	L	0.0000
199	Q	L	-1.2367
200	G	L	-0.3609
201	L	L	0.2714
202	S	L	-0.1887
203	S	L	-0.2884
204	P	L	-0.1522
205	V	L	0.3820
206	T	L	-0.1710
207	K	L	-0.8811
208	S	L	-0.2230
209	F	L	0.0838
210	N	L	-1.1318
211	R	L	-0.8641
212	G	L	-0.9304
213	E	L	-1.8880
214	C	L	-0.2048
1	D	A	0.0000
2	A	A	0.0000
3	E	A	0.0000
4	F	A	0.1059
5	R	A	-0.6330
6	H	A	-1.0790