Project name: 14-8pig [mutate: FH138Q, PH140L, AH219D]

Chain sequence(s)	H: QVQLQESGPGLVKPSETLSLTCTVSGFSPTNFGVHWIRQPPGKGLEWIGVIWAGGSTNYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARDGYNGYYYTMAYWGQGTSVTVSS; L: DIQMTQSPSSLSASVGDRVTITCSASSTVSFMNWYQQKPGKAPKLLIYSTSILASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQRSSLPLITFGAGTKLEIKRTV
Distance of aggregation	5 Å
Dynamic mode	No
Mutated residues	FH138Q, PH140L, AH219D
Energy difference between WT (input) and mutated protein (by FoldX)	-2.51201 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.18
• Maximal score value: 1.7598
• Average score: -0.211
• Total score value: -48.5329

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	D	L	-1.7372
2	I	L	-0.2682
3	Q	L	-1.1444
4	M	L	0.0000
5	T	L	-0.0709
6	Q	L	0.0000
7	S	L	-0.2193
8	P	L	-0.2062
9	S	L	-0.2830
10	S	L	-0.3558
11	L	L	0.1450
12	S	L	-0.3049
13	A	L	-0.0402
14	S	L	-0.0380
15	V	L	1.0495
16	G	L	-0.4321
17	D	L	-1.3336
18	R	L	-2.0241
19	V	L	0.0000
20	T	L	-0.0642
21	I	L	0.0000
22	T	L	-0.0280
23	C	L	0.0000
24	S	L	-0.2385
25	A	L	-0.0365
26	S	L	-0.1976
27	S	L	-0.1849
28	T	L	-0.0527
29	V	L	0.1716
31	S	L	-0.1075
32	F	L	0.3532
33	M	L	0.0000
34	N	L	0.0000
35	W	L	0.0000
36	Y	L	0.0000
37	Q	L	-0.1460
38	Q	L	0.0000
39	K	L	-0.5762
40	P	L	-0.4366
41	G	L	-0.8255
42	K	L	-1.7833
43	A	L	-0.3113
44	P	L	0.0000
45	K	L	-1.7000
46	L	L	0.0000
47	L	L	0.0000
48	I	L	0.0000
49	Y	L	0.1512
50	S	L	0.0535
51	T	L	-0.0541
52	S	L	-0.0758
53	I	L	0.8571
54	L	L	0.7041
55	A	L	0.0000
56	S	L	-0.2981
57	G	L	-0.5052
58	V	L	0.0000
59	P	L	-0.2049
60	S	L	-0.3191
61	R	L	-0.4389
62	F	L	0.0000
63	S	L	-0.1676
64	G	L	-0.1550
65	S	L	-0.2871
66	G	L	-0.5008
67	S	L	-0.3792
68	G	L	-0.5097
69	T	L	-0.3559
70	D	L	-1.3551
71	F	L	0.0000
72	T	L	-0.0370
73	L	L	0.0000
74	T	L	-0.0188
75	I	L	0.0000
76	S	L	-0.3848
77	S	L	-0.1774
78	L	L	0.0000
79	Q	L	-0.4839
80	P	L	-0.6047
81	E	L	-1.8538
82	D	L	0.0000
83	F	L	0.7800
84	A	L	0.0000
85	T	L	-0.1917
86	Y	L	0.0000
87	Y	L	0.0000
88	C	L	0.0000
89	Q	L	0.0000
90	Q	L	0.0000
91	R	L	0.0000
92	S	L	-0.0805
93	S	L	-0.0743
94	L	L	0.2817
95	P	L	-0.0004
96	L	L	0.3010
97	I	L	0.0000
98	T	L	0.0338
99	F	L	0.2183
100	G	L	0.0000
101	A	L	0.0585
102	G	L	0.0000
103	T	L	0.0000
104	K	L	-1.4041
105	L	L	0.0000
106	E	L	-1.0705
107	I	L	-0.2348
108	K	L	-2.0014
109	R	L	-2.1800
110	T	L	-0.1026
111	V	L	1.7598
112	Q	H	-1.1983
113	V	H	0.0000
114	Q	H	-1.1983
115	L	H	0.0000
116	Q	H	-1.2305
117	E	H	-0.4348
118	S	H	-0.3079
119	G	H	-0.4122
120	P	H	-0.3210
121	G	H	-0.1457
122	L	H	1.3456
123	V	H	0.0000
124	K	H	-1.2216
125	P	H	-0.3567
126	S	H	-0.5252
127	E	H	-1.4731
128	T	H	-0.3285
129	L	H	0.0000
130	S	H	-0.2333
131	L	H	0.0000
132	T	H	-0.0318
133	C	H	0.0000
134	T	H	-0.0808
135	V	H	0.0000
136	S	H	-0.1963
137	G	H	-0.3379
138	Q	H	-0.2573	mutated: FH138Q
139	S	H	0.0371
140	L	H	1.4998	mutated: PH140L
141	T	H	-0.0051
142	N	H	-1.2397
143	F	H	0.0021
144	G	H	0.0000
145	V	H	0.0000
146	H	H	0.0000
147	W	H	0.0000
148	I	H	0.0000
149	R	H	0.0000
150	Q	H	-0.1091
151	P	H	-0.1033
152	P	H	-0.3463
153	G	H	-0.8202
154	K	H	-1.8167
155	G	H	-0.4578
156	L	H	0.0258
157	E	H	-0.4600
158	W	H	0.0000
159	I	H	0.0000
160	G	H	0.0000
161	V	H	0.0000
162	I	H	0.0000
163	W	H	0.3256
164	A	H	0.0252
165	G	H	-0.5142
166	G	H	-0.4372
167	S	H	-0.2736
168	T	H	-0.1513
169	N	H	-0.3982
170	Y	H	0.3469
171	N	H	0.0000
172	P	H	-0.2775
173	S	H	-0.1733
174	L	H	0.0000
175	K	H	-1.7383
176	S	H	-0.5846
177	R	H	-0.4102
178	V	H	0.0000
179	T	H	-0.0527
180	I	H	0.0000
181	S	H	0.0483
182	V	H	0.9287
183	D	H	-0.9009
184	T	H	-0.3058
185	S	H	-0.5317
186	K	H	-1.8779
187	N	H	-1.1693
188	Q	H	-0.6509
189	F	H	0.0000
190	S	H	-0.0847
191	L	H	0.0000
192	K	H	-1.3604
193	L	H	0.0000
194	S	H	-0.1533
195	S	H	-0.2666
196	V	H	0.0000
197	T	H	-0.0407
198	A	H	0.0596
199	A	H	0.0689
200	D	H	0.0000
201	T	H	-0.0165
202	A	H	0.0000
203	V	H	0.3066
204	Y	H	0.0000
205	Y	H	0.0000
206	C	H	0.0000
207	A	H	0.0000
208	R	H	0.0000
209	D	H	0.0000
210	G	H	0.0000
211	Y	H	1.1442
212	N	H	-1.0663
213	G	H	-0.6412
214	Y	H	0.3378
215	Y	H	0.4961
216	Y	H	0.8398
217	T	H	0.0000
218	M	H	0.0000
219	D	H	-0.4821	mutated: AH219D
220	Y	H	0.1658
221	W	H	0.1683
222	G	H	0.0000
223	Q	H	-1.1983
224	G	H	0.0000
225	T	H	-0.0499
226	S	H	-0.1584
227	V	H	0.0000
228	T	H	0.1129
229	V	H	0.0000
230	S	H	-0.1184
231	S	H	-0.3486