Project name: SH3_L86V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA86V
Energy difference between WT (input) and mutated protein (by FoldX)	2.47095 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7361
• Average score: -1.0441
• Total score value: -59.5135

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.3351
86	V	A	0.3575	mutated: LA86V
87	F	A	0.6780
88	V	A	0.2750
89	A	A	0.0000
90	L	A	-0.1573
91	Y	A	-0.5736
92	D	A	-2.5594
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7378
105	K	A	-2.4165
106	G	A	-1.4786
107	E	A	-1.3808
108	K	A	-1.0045
109	F	A	0.0000
110	Q	A	-1.2374
111	I	A	-0.3198
112	L	A	0.2392
113	N	A	-0.7864
114	S	A	-1.1032
115	S	A	-1.5572
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0970
121	E	A	-1.2218
122	A	A	0.0000
123	R	A	-2.4163
124	S	A	0.0000
125	L	A	-0.3932
126	T	A	-0.6455
127	T	A	-0.9599
128	G	A	-1.6278
129	E	A	-2.4332
130	T	A	-1.9622
131	G	A	-1.5802
132	Y	A	-0.9024
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9195
136	N	A	-1.1501
137	Y	A	-0.1235
138	V	A	0.0000
139	A	A	0.3844
140	P	A	0.6678
141	V	A	1.7361