Project name: SH3_E121Y

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA121Y
Energy difference between WT (input) and mutated protein (by FoldX)	-2.007 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.093
• Maximal score value: 1.7964
• Average score: -0.8578
• Total score value: -48.8933

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9250
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6190
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.0930
99	D	A	-2.8987
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1958
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6420
109	F	A	0.0000
110	Q	A	-0.3942
111	I	A	0.1650
112	L	A	0.3655
113	N	A	-0.6880
114	S	A	-1.0240
115	S	A	-1.5291
116	E	A	-2.4515
117	G	A	-2.0459
118	D	A	-2.3397
119	W	A	-0.8638
120	W	A	-0.5760
121	Y	A	-0.1370	mutated: EA121Y
122	A	A	0.0000
123	R	A	-1.4875
124	S	A	0.0000
125	L	A	0.0610
126	T	A	-0.4730
127	T	A	-0.8184
128	G	A	-1.3479
129	E	A	-2.2205
130	T	A	-1.4201
131	G	A	0.0000
132	Y	A	-0.4079
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.7862
136	N	A	-1.1500
137	Y	A	-0.1241
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964