Project name: SH3_Y132N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA132N
Energy difference between WT (input) and mutated protein (by FoldX)	2.3078 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.2854
• Maximal score value: 1.7964
• Average score: -1.0487
• Total score value: -59.7749

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9250
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.7145
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1860
99	D	A	-3.2854
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2938
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6420
109	F	A	0.0000
110	Q	A	-0.5698
111	I	A	-0.2487
112	L	A	-0.0769
113	N	A	-1.1068
114	S	A	-1.3394
115	S	A	-1.6376
116	E	A	-2.6124
117	G	A	-2.1715
118	D	A	-2.5614
119	W	A	-1.3925
120	W	A	-1.5048
121	E	A	-1.9160
122	A	A	0.0000
123	R	A	-1.9113
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3573
129	E	A	-2.2402
130	T	A	-1.9452
131	G	A	-1.9990
132	N	A	-1.7416	mutated: YA132N
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0667
136	N	A	-1.1465
137	Y	A	-0.1216
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964