Project name: SH3_N113G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	NA113G
Energy difference between WT (input) and mutated protein (by FoldX)	2.46559 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1017
• Maximal score value: 1.7964
• Average score: -0.9378
• Total score value: -53.4524

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9250
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6246
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1017
99	D	A	-3.0529
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2047
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6420
109	F	A	0.0000
110	Q	A	-0.4603
111	I	A	0.0611
112	L	A	0.3633
113	G	A	-0.5394	mutated: NA113G
114	S	A	-1.0177
115	S	A	-1.4966
116	E	A	-2.4941
117	G	A	-2.0983
118	D	A	-2.4188
119	W	A	-1.0756
120	W	A	-0.9762
121	E	A	-1.0987
122	A	A	0.0000
123	R	A	-1.7173
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2400
130	T	A	-1.7020
131	G	A	-1.5185
132	Y	A	-0.8478
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9317
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964