Project name: SH3_W120D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA120D
Energy difference between WT (input) and mutated protein (by FoldX)	3.85173 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8484
• Average score: -0.9433
• Total score value: -53.7683

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5623
86	L	A	0.8784
87	F	A	1.0801
88	V	A	0.5060
89	A	A	0.0000
90	L	A	-0.1326
91	Y	A	-0.5743
92	D	A	-2.5596
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4057
106	G	A	-1.4400
107	E	A	-1.2848
108	K	A	-0.5896
109	F	A	0.0000
110	Q	A	-0.4639
111	I	A	-0.0344
112	L	A	0.1182
113	N	A	-0.9590
114	S	A	-1.3086
115	S	A	-1.6579
116	E	A	-2.6100
117	G	A	-2.1814
118	D	A	-2.5130
119	W	A	-1.2090
120	D	A	0.0000	mutated: WA120D
121	E	A	-1.2429
122	A	A	0.0000
123	R	A	-1.7213
124	S	A	0.0000
125	L	A	0.0749
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6868
131	G	A	-1.5224
132	Y	A	-0.9310
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9906
136	N	A	-1.1852
137	Y	A	-0.1166
138	V	A	0.0000
139	A	A	0.4621
140	P	A	0.8415
141	V	A	1.8484