Project name: SH3_Y91D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA91D
Energy difference between WT (input) and mutated protein (by FoldX)	1.04718 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.5213
• Maximal score value: 1.7964
• Average score: -1.099
• Total score value: -62.6439

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9866
88	V	A	0.1846
89	A	A	0.0000
90	L	A	-1.0218
91	D	A	-3.1706	mutated: YA91D
92	D	A	-3.5213
93	Y	A	-2.4402
94	E	A	-2.8576
95	A	A	-2.5833
96	R	A	-2.9852
97	T	A	-2.6609
98	E	A	-3.1029
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1304
103	F	A	0.0000
104	H	A	-3.2271
105	K	A	-3.4022
106	G	A	-1.8547
107	E	A	-1.5620
108	K	A	-0.5894
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1008
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4992
132	Y	A	-0.8674
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9145
136	N	A	-1.3763
137	Y	A	-0.6460
138	V	A	0.0000
139	A	A	0.5331
140	P	A	0.7756
141	V	A	1.7964