Project name: SOD1 [mutate: KA3E]

Chain sequence(s)	A: ATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ; F: ATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ
Distance of aggregation	5 Å
Dynamic mode	No
Mutated residues	KA3E
Energy difference between WT (input) and mutated protein (by FoldX)	2.60068 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.262
• Maximal score value: 1.2677
• Average score: -0.3981
• Total score value: -121.8259

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	A	A	-0.2378
2	T	A	-0.1320
3	E	A	-0.6587	mutated: KA3E
4	A	A	0.0000
5	V	A	0.0000
6	C	A	0.0000
7	V	A	0.3004
8	L	A	0.0000
9	K	A	-1.8788
10	G	A	-0.9050
11	D	A	-1.9245
12	G	A	-0.8441
13	P	A	-0.3419
14	V	A	0.0000
15	Q	A	-1.3934
16	G	A	-0.1562
17	I	A	0.6832
18	I	A	0.0000
19	N	A	-0.2795
20	F	A	0.0000
21	E	A	-0.6163
22	Q	A	0.0000
23	K	A	-2.0240
24	E	A	-2.1777
25	S	A	-0.7903
26	N	A	-1.3225
27	G	A	-0.5031
28	P	A	-0.1460
29	V	A	0.0000
30	K	A	-1.4410
31	V	A	0.0000
32	W	A	0.2601
33	G	A	-0.0487
34	S	A	-0.3129
35	I	A	0.0000
36	K	A	-1.5858
37	G	A	-0.5245
38	L	A	0.0000
39	T	A	-0.4005
40	E	A	-1.8459
41	G	A	-0.1787
42	L	A	1.1790
43	H	A	0.0000
44	G	A	0.0000
45	F	A	0.0000
46	H	A	0.0000
47	V	A	0.0000
48	H	A	0.0000
49	E	A	-0.2308
50	F	A	0.1582
51	G	A	0.0000
52	D	A	-0.3115
53	N	A	-0.3969
54	T	A	-0.0872
55	A	A	0.0247
56	G	A	-0.1594
57	C	A	0.0335
58	T	A	-0.0657
59	S	A	-0.0577
60	A	A	0.0000
61	G	A	-0.1428
62	P	A	-0.2223
63	H	A	0.0000
64	F	A	0.0000
65	N	A	-0.3216
66	P	A	0.0364
67	L	A	1.0914
68	S	A	-0.1090
69	R	A	-0.9038
70	K	A	-1.8017
71	H	A	0.0000
72	G	A	0.0000
73	G	A	0.0000
74	P	A	-0.3591
75	K	A	-1.9081
76	D	A	-1.7408
77	E	A	-2.1581
78	E	A	-1.0595
79	R	A	0.0000
80	H	A	0.0000
81	V	A	0.0000
82	G	A	0.0000
83	D	A	0.0000
84	L	A	0.0000
85	G	A	0.0000
86	N	A	-0.2681
87	V	A	0.0000
88	T	A	0.0909
89	A	A	0.0000
90	D	A	-1.3662
91	K	A	-2.2216
92	D	A	-2.0979
93	G	A	0.0000
94	V	A	-0.0040
95	A	A	0.0000
96	D	A	-1.7993
97	V	A	0.0000
98	S	A	-0.1156
99	I	A	-0.0592
100	E	A	-1.9146
101	D	A	0.0000
102	S	A	-0.1151
103	V	A	0.5735
104	I	A	0.0000
105	S	A	-0.0080
106	L	A	0.0000
107	S	A	-0.2837
108	G	A	-0.8381
109	D	A	-1.9133
110	H	A	-0.5090
111	C	A	0.0527
112	I	A	0.0000
113	I	A	0.0000
114	G	A	0.0000
115	R	A	-0.2110
116	T	A	0.0000
117	L	A	0.0000
118	V	A	0.0000
119	V	A	0.0000
120	H	A	0.0000
121	E	A	-1.5521
122	K	A	-1.4434
123	A	A	-0.0704
124	D	A	0.0000
125	D	A	-0.3688
126	L	A	0.1801
127	G	A	-0.3614
128	K	A	-1.7838
129	G	A	-0.7614
130	G	A	-0.7064
131	N	A	-1.3923
132	E	A	-2.2620
133	E	A	-1.9352
134	S	A	0.0000
135	T	A	-0.3230
136	K	A	-1.8513
137	T	A	-0.3239
138	G	A	0.0000
139	N	A	-0.2441
140	A	A	0.0000
141	G	A	-0.3489
142	S	A	-0.3636
143	R	A	-0.4554
144	L	A	0.3218
145	A	A	0.0000
146	C	A	0.0000
147	G	A	-0.0088
148	V	A	0.1950
149	I	A	0.0000
150	G	A	0.0000
151	I	A	0.3600
152	A	A	-0.1527
153	Q	A	-1.1971
1	A	F	-0.2256
2	T	F	-0.1648
3	K	F	-0.8600
4	A	F	0.0000
5	V	F	0.0000
6	C	F	0.0000
7	V	F	0.3169
8	L	F	0.0000
9	K	F	-1.8535
10	G	F	-0.7939
11	D	F	-1.9325
12	G	F	-0.8731
13	P	F	-0.5983
14	V	F	0.0000
15	Q	F	-1.2990
16	G	F	-0.1316
17	I	F	0.6954
18	I	F	0.0000
19	N	F	-0.2571
20	F	F	0.0000
21	E	F	-0.9923
22	Q	F	0.0000
23	K	F	-2.0314
24	E	F	-2.1788
25	S	F	-0.7947
26	N	F	-1.3257
27	G	F	-0.3294
28	P	F	-0.1384
29	V	F	0.0000
30	K	F	-1.2335
31	V	F	0.0000
32	W	F	0.2460
33	G	F	-0.0442
34	S	F	-0.3183
35	I	F	0.0000
36	K	F	-1.7564
37	G	F	-0.7833
38	L	F	0.0000
39	T	F	-0.4036
40	E	F	-1.8479
41	G	F	-0.1746
42	L	F	1.2677
43	H	F	0.0000
44	G	F	0.0000
45	F	F	0.0000
46	H	F	0.0000
47	V	F	0.0000
48	H	F	0.0000
49	E	F	-0.2711
50	F	F	0.1392
51	G	F	0.0000
52	D	F	-0.3269
53	N	F	-0.3819
54	T	F	-0.0804
55	A	F	0.0064
56	G	F	-0.2494
57	C	F	0.0273
58	T	F	-0.0624
59	S	F	-0.0521
60	A	F	0.0000
61	G	F	-0.1327
62	P	F	-0.2286
63	H	F	0.0000
64	F	F	0.0000
65	N	F	-0.2857
66	P	F	-0.0075
67	L	F	0.9112
68	S	F	-0.0991
69	R	F	-0.6675
70	K	F	-1.5427
71	H	F	0.0000
72	G	F	0.0000
73	G	F	0.0000
74	P	F	-0.3905
75	K	F	-1.8038
76	D	F	-1.1218
77	E	F	-2.1850
78	E	F	-1.8266
79	R	F	0.0000
80	H	F	0.0000
81	V	F	0.0000
82	G	F	0.0000
83	D	F	0.0000
84	L	F	0.0000
85	G	F	0.0000
86	N	F	-0.2945
87	V	F	0.0000
88	T	F	0.1045
89	A	F	0.0000
90	D	F	-1.3946
91	K	F	-2.2228
92	D	F	-2.0959
93	G	F	0.0000
94	V	F	-0.0103
95	A	F	0.0000
96	D	F	-1.8001
97	V	F	0.0000
98	S	F	-0.1173
99	I	F	-0.0626
100	E	F	-1.8893
101	D	F	0.0000
102	S	F	-0.1455
103	V	F	0.4072
104	I	F	0.0000
105	S	F	-0.0109
106	L	F	0.0000
107	S	F	-0.2010
108	G	F	-0.8229
109	D	F	-1.9266
110	H	F	-0.5809
111	C	F	0.0389
112	I	F	0.0000
113	I	F	0.2054
114	G	F	0.0000
115	R	F	-0.2326
116	T	F	0.0000
117	L	F	0.0000
118	V	F	0.0000
119	V	F	0.0000
120	H	F	0.0000
121	E	F	-1.7897
122	K	F	-1.4684
123	A	F	-0.0625
124	D	F	0.0000
125	D	F	-0.4768
126	L	F	0.1342
127	G	F	-0.3216
128	K	F	-1.7792
129	G	F	-0.7812
130	G	F	-0.6773
131	N	F	-1.2179
132	E	F	-2.2018
133	E	F	-1.6289
134	S	F	0.0000
135	T	F	-0.3238
136	K	F	-1.7066
137	T	F	-0.3308
138	G	F	0.0000
139	N	F	-0.2709
140	A	F	0.0000
141	G	F	-0.3790
142	S	F	-0.3816
143	R	F	-0.5302
144	L	F	0.2695
145	A	F	0.0000
146	C	F	0.0000
147	G	F	0.0000
148	V	F	0.1957
149	I	F	0.0000
150	G	F	0.0000
151	I	F	0.3961
152	A	F	0.0000
153	Q	F	-1.1983