Project name: SH3_Y93V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA93V
Energy difference between WT (input) and mutated protein (by FoldX)	5.41985 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1557
• Maximal score value: 1.7964
• Average score: -1.0149
• Total score value: -57.8501

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9396
88	V	A	0.4528
89	A	A	0.0000
90	L	A	-0.0959
91	Y	A	-0.5250
92	D	A	-2.5129
93	V	A	-1.8781	mutated: YA93V
94	E	A	-2.6449
95	A	A	-2.6751
96	R	A	-3.0211
97	T	A	-2.7104
98	E	A	-3.1557
99	D	A	-3.1316
100	D	A	-2.2481
101	L	A	0.0000
102	S	A	-2.2299
103	F	A	0.0000
104	H	A	-2.7145
105	K	A	-2.3773
106	G	A	-1.4574
107	E	A	-1.3091
108	K	A	-0.6430
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0574
112	L	A	0.1425
113	N	A	-0.8974
114	S	A	-1.1933
115	S	A	-1.6047
116	E	A	-2.5725
117	G	A	-2.1564
118	D	A	-2.4824
119	W	A	-1.1877
120	W	A	-1.1155
121	E	A	-1.1984
122	A	A	0.0000
123	R	A	-1.7161
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.7192
131	G	A	-1.5703
132	Y	A	-0.9722
133	I	A	0.0000
134	P	A	-0.5856
135	S	A	-0.9120
136	N	A	-1.1035
137	Y	A	0.0039
138	V	A	0.0000
139	A	A	0.4454
140	P	A	0.7757
141	V	A	1.7964