Project name: SH3_Y137D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA137D
Energy difference between WT (input) and mutated protein (by FoldX)	2.56639 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -1.0103
• Total score value: -57.5854

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.8506
88	V	A	0.3341
89	A	A	0.0000
90	L	A	-0.3459
91	Y	A	-0.7229
92	D	A	-2.6359
93	Y	A	-2.0034
94	E	A	-2.6259
95	A	A	-2.6189
96	R	A	-2.9807
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1931
103	F	A	0.0000
104	H	A	-2.7183
105	K	A	-2.4834
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0569
112	L	A	0.1429
113	N	A	-0.8883
114	S	A	-1.1800
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1378
118	D	A	-2.4528
119	W	A	-1.1839
120	W	A	-1.0641
121	E	A	-1.1573
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4995
132	Y	A	-0.8747
133	I	A	0.0000
134	P	A	-0.7706
135	S	A	-1.1390
136	N	A	-1.4653
137	D	A	-0.7572	mutated: YA137D
138	V	A	0.0000
139	A	A	0.2817
140	P	A	0.7757
141	V	A	1.7964