Project name: rbd2 [mutate: KC417Y, AC475N, QC498D, GC447D, LC452R, QC493K]

Chain sequence(s)	C: TNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGP
Distance of aggregation	5 Å
Dynamic mode	No
Mutated residues	KC417Y, AC475N, QC498D, GC447D, LC452R, QC493K
Energy difference between WT (input) and mutated protein (by FoldX)	7.4018 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.1282
• Maximal score value: 1.738
• Average score: -0.2129
• Total score value: -41.5084

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
333	T	C	-0.3035
334	N	C	-0.9920
335	L	C	1.3270
336	C	C	0.3527
337	P	C	-0.0218
338	F	C	0.1387
339	G	C	-0.6386
340	E	C	-1.8831
341	V	C	0.0000
342	F	C	0.0000
343	N	C	-1.2701
344	A	C	-0.2307
345	T	C	-0.4052
346	R	C	-1.8570
347	F	C	0.0000
348	A	C	0.0051
349	S	C	-0.0204
350	V	C	0.0000
351	Y	C	0.1373
352	A	C	0.0336
353	W	C	0.0000
354	N	C	-1.4285
355	R	C	-1.2266
356	K	C	-1.3025
357	R	C	-1.9796
358	I	C	0.0000
359	S	C	-0.3248
360	N	C	-1.2898
361	C	C	0.0000
362	V	C	0.7176
363	A	C	-0.1323
364	D	C	-1.4741
365	Y	C	0.0000
366	S	C	0.1350
367	V	C	1.7380
368	L	C	0.0000
369	Y	C	1.0797
370	N	C	-1.0430
371	S	C	-0.2958
372	A	C	0.0061
373	S	C	-0.1519
374	F	C	0.2138
375	S	C	-0.1516
376	T	C	0.0486
377	F	C	0.3448
378	K	C	-1.1161
379	C	C	0.0131
380	Y	C	0.4301
381	G	C	-0.3033
382	V	C	0.2238
383	S	C	-0.0363
384	P	C	-0.0605
385	T	C	-0.3870
386	K	C	-1.7129
387	L	C	0.0000
388	N	C	-0.8262
389	D	C	-1.7592
390	L	C	0.3638
391	C	C	0.4098
392	F	C	0.2746
393	T	C	0.0000
394	N	C	-0.1758
395	V	C	0.0000
396	Y	C	-0.0700
397	A	C	0.0000
398	D	C	0.0000
399	S	C	0.0000
400	F	C	0.0000
401	V	C	0.0000
402	I	C	0.0000
403	R	C	-0.3108
404	G	C	-0.4141
405	D	C	-1.3666
406	E	C	0.0000
407	V	C	-0.1351
408	R	C	-1.8319
409	Q	C	-0.4739
410	I	C	0.0000
411	A	C	-0.0030
412	P	C	-0.3343
413	G	C	-0.5572
414	Q	C	-0.5419
415	T	C	-0.1697
416	G	C	0.0621
417	Y	C	0.8818	mutated: KC417Y
418	I	C	0.0000
419	A	C	0.0000
420	D	C	-0.3267
421	Y	C	0.1626
422	N	C	0.0000
423	Y	C	0.0000
424	K	C	-0.5408
425	L	C	0.0000
426	P	C	-0.3687
427	D	C	-2.1282
428	D	C	-2.1159
429	F	C	0.0000
430	T	C	-0.1233
431	G	C	0.0000
432	C	C	0.0000
433	V	C	0.0000
434	I	C	0.0000
435	A	C	0.0000
436	W	C	0.0631
437	N	C	-0.4062
438	S	C	0.0000
439	N	C	-0.4797
440	N	C	-1.0368
441	L	C	1.3163
442	D	C	0.0000
443	S	C	-0.3210
444	K	C	-1.2772
445	V	C	1.4037
446	G	C	-0.2202
447	D	C	-0.7732	mutated: GC447D
448	N	C	-0.1520
449	Y	C	1.1042
450	N	C	-0.7881
451	Y	C	0.0000
452	R	C	-0.8680	mutated: LC452R
453	Y	C	0.0000
454	R	C	0.0000
455	L	C	0.2452
456	F	C	0.3316
457	R	C	-0.6225
458	K	C	-1.8083
459	S	C	-0.7586
460	N	C	-1.3370
461	L	C	0.0000
462	K	C	-1.7192
463	P	C	-0.3625
464	F	C	0.5104
465	E	C	-0.5867
466	R	C	-1.4662
467	D	C	0.0000
468	I	C	1.2304
469	S	C	0.0941
470	T	C	-0.4259
471	E	C	-1.7787
472	I	C	-0.0009
473	Y	C	0.1359
474	Q	C	-0.9814
475	N	C	-1.3862	mutated: AC475N
476	G	C	-0.5115
477	S	C	-0.2572
478	T	C	-0.1565
479	P	C	-0.2530
480	C	C	-0.2006
481	N	C	-1.3449
482	G	C	-0.3794
483	V	C	1.3506
484	E	C	-1.5547
485	G	C	-0.3670
486	F	C	1.7023
487	N	C	-0.6713
488	C	C	-0.2878
489	Y	C	0.6452
490	F	C	1.3082
491	P	C	0.0000
492	L	C	-0.1501
493	K	C	-1.6570	mutated: QC493K
494	S	C	-0.5352
495	Y	C	0.0000
496	G	C	-0.1008
497	F	C	0.0000
498	D	C	-0.4331	mutated: QC498D
499	P	C	-0.2217
500	T	C	-0.1527
501	N	C	-0.4007
502	G	C	-0.1925
503	V	C	1.6505
504	G	C	0.2037
505	Y	C	0.3780
506	Q	C	-0.0560
507	P	C	0.0000
508	Y	C	0.0832
509	R	C	0.0000
510	V	C	0.0000
511	V	C	0.0000
512	V	C	0.0000
513	L	C	0.0000
514	S	C	-0.0208
515	F	C	0.0000
516	E	C	-0.4525
517	L	C	1.6982
518	L	C	1.6525
519	H	C	-0.7005
520	A	C	-0.2045
521	P	C	-0.2456
522	A	C	0.0010
523	T	C	-0.0147
524	V	C	0.0000
525	C	C	0.3766
526	G	C	-0.1342
527	P	C	-0.2677