Project name: 5E10

Chain sequence(s)	H: EVQLVQSGAEVKKPGESLKISCKGSGYSFTDYWIGWVRQMPGKGLEWMGIIYPGDSDTRYSPSFQGQVTISADKSISTAYLQWSSLKASDTAVYYCARVGRFASHQLDYWGQGTLVTVSSASTKGPSVFPLAPGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP; C: QLLFNKTKSVEFTFGNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYR; L: EIVLTQSPATLSLSPGERATLSCRASQSVNNRLAWYQQKPGQAPRLLIHWASTRAIGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQGASWPFTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG
Distance of aggregation	10 Å
Dynamic mode	No

• Minimal score value: -3.5806
• Maximal score value: 1.4937
• Average score: -0.6689
• Total score value: -360.524

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	E	H	-2.3041
2	V	H	0.0000
3	Q	H	-1.8322
4	L	H	0.0000
5	V	H	-0.1322
6	Q	H	0.0000
7	S	H	-0.5595
8	G	H	-0.3090
9	A	H	0.2142
10	E	H	0.0760
11	V	H	0.7779
12	K	H	-0.9122
13	K	H	-1.7149
14	P	H	-1.9645
15	G	H	-1.8928
16	E	H	-2.0566
17	S	H	-1.7570
18	L	H	0.0000
19	K	H	-1.9193
20	I	H	0.0000
21	S	H	-0.6968
22	C	H	0.0000
23	K	H	-1.0720
24	G	H	0.0000
25	S	H	-1.1150
26	G	H	-1.5139
27	Y	H	-0.7475
28	S	H	-0.2450
29	F	H	0.0000
30	T	H	-0.9484
31	D	H	-0.3772
32	Y	H	-0.0639
33	W	H	0.0000
34	I	H	0.0000
35	G	H	0.0000
36	W	H	0.0000
37	V	H	0.0000
38	R	H	-0.5773
39	Q	H	-0.7262
40	M	H	-0.7518
41	P	H	-0.9494
42	G	H	-1.4306
43	K	H	-2.3215
44	G	H	-1.5519
45	L	H	0.0000
46	E	H	-0.9023
47	W	H	0.0000
48	M	H	0.0000
49	G	H	0.0000
50	I	H	0.0000
51	I	H	0.0000
52	Y	H	0.0000
53	P	H	0.0000
54	G	H	-1.5874
55	D	H	-2.4112
56	S	H	-1.7065
57	D	H	-1.2383
58	T	H	-0.6450
59	R	H	-0.5070
60	Y	H	-0.5829
61	S	H	0.0000
62	P	H	-1.1617
63	S	H	-0.9509
64	F	H	0.0000
65	Q	H	-1.9380
66	G	H	-1.6778
67	Q	H	-1.9950
68	V	H	0.0000
69	T	H	-0.9813
70	I	H	0.0000
71	S	H	-0.4385
72	A	H	-1.0390
73	D	H	-1.4337
74	K	H	-1.6985
75	S	H	-0.2401
76	I	H	0.8932
77	S	H	-0.4059
78	T	H	0.0000
79	A	H	0.0000
80	Y	H	-0.7279
81	L	H	0.0000
82	Q	H	-1.5182
83	W	H	0.0000
84	S	H	-1.3949
85	S	H	-1.6782
86	L	H	0.0000
87	K	H	-2.4280
88	A	H	-1.2764
89	S	H	-0.8028
90	D	H	-0.8614
91	T	H	-0.2376
92	A	H	0.0000
93	V	H	0.4277
94	Y	H	0.0000
95	Y	H	0.0000
96	C	H	0.0000
97	A	H	0.0000
98	R	H	-0.2089
99	V	H	0.0000
100	G	H	0.0000
101	R	H	0.1501
102	F	H	0.8880
103	A	H	0.0000
104	S	H	0.0000
105	H	H	0.0000
106	Q	H	0.0000
107	L	H	0.0000
108	D	H	-0.1527
109	Y	H	-0.1061
110	W	H	-0.3895
111	G	H	0.0000
112	Q	H	-1.2418
113	G	H	-0.4714
114	T	H	0.0000
115	L	H	0.8099
116	V	H	0.0000
117	T	H	0.0000
118	V	H	0.0000
119	S	H	-0.7593
120	S	H	-0.9121
121	A	H	-0.4420
122	S	H	-0.5065
123	T	H	-0.4825
124	K	H	-1.1336
125	G	H	-1.3449
126	P	H	0.0000
127	S	H	-0.4147
128	V	H	0.0000
129	F	H	0.0000
130	P	H	-1.2273
131	L	H	0.0000
132	A	H	-0.8720
133	P	H	-0.4841
141	G	H	-0.6424
142	T	H	-0.4823
143	A	H	-0.2733
144	A	H	0.0000
145	L	H	0.0000
146	G	H	0.0000
147	C	H	0.0000
148	L	H	0.0000
149	V	H	0.0000
150	K	H	0.0000
151	D	H	-0.3972
152	Y	H	0.0000
153	F	H	0.0000
154	P	H	0.0000
155	E	H	-0.7233
156	P	H	-0.8914
157	V	H	-0.5720
158	T	H	-0.6575
159	V	H	-0.2619
160	S	H	-0.5044
161	W	H	0.0000
162	N	H	-0.8749
163	S	H	-0.7309
164	G	H	-0.5725
165	A	H	-0.2691
166	L	H	-0.0519
167	T	H	-0.1887
168	S	H	-0.1793
169	G	H	-0.2055
170	V	H	0.1356
171	H	H	-0.1342
172	T	H	-0.0174
173	F	H	0.0000
174	P	H	-0.4231
175	A	H	0.0500
176	V	H	0.3028
177	L	H	1.0092
178	Q	H	0.1887
179	S	H	-0.0906
180	S	H	-0.2251
181	G	H	0.0081
182	L	H	0.0415
183	Y	H	0.2936
184	S	H	0.0000
185	L	H	0.0000
186	S	H	0.0000
187	S	H	0.0000
188	V	H	0.0000
189	V	H	0.0000
190	T	H	-0.1513
191	V	H	0.0000
192	P	H	-0.6044
193	S	H	-0.5425
194	S	H	-0.6439
195	S	H	-0.6702
196	L	H	-0.9045
197	G	H	-1.0392
198	T	H	-0.8016
199	Q	H	-1.4054
200	T	H	-1.2219
201	Y	H	0.0000
202	I	H	-1.5392
203	C	H	0.0000
204	N	H	-1.7155
205	V	H	0.0000
206	N	H	-2.0299
207	H	H	0.0000
208	K	H	-2.7712
209	P	H	-1.6521
210	S	H	-1.8082
211	N	H	-2.6050
212	T	H	-2.0967
213	K	H	-2.8060
214	V	H	-1.8162
215	D	H	-2.7802
216	K	H	-2.5229
217	K	H	-2.7299
218	V	H	0.0000
219	E	H	-2.5495
220	P	H	-1.1958
1	E	L	-1.5247
2	I	L	0.0000
3	V	L	0.6576
4	L	L	0.0000
5	T	L	-0.6347
6	Q	L	0.0000
7	S	L	-0.6217
8	P	L	-0.2844
9	A	L	-0.4150
10	T	L	-0.3721
11	L	L	-0.1942
12	S	L	-0.4257
13	L	L	-0.7528
14	S	L	-1.1906
15	P	L	-1.6425
16	G	L	-1.7834
17	E	L	-2.2749
18	R	L	-2.6672
19	A	L	0.0000
20	T	L	-0.5434
21	L	L	0.0000
22	S	L	-0.8505
23	C	L	0.0000
24	R	L	-2.0293
25	A	L	0.0000
26	S	L	-1.0024
27	Q	L	-1.6904
28	S	L	-1.2498
29	V	L	0.0000
30	N	L	0.0000
31	N	L	-0.3528
32	R	L	0.0000
33	L	L	0.0000
34	A	L	0.0000
35	W	L	0.0000
36	Y	L	0.0000
37	Q	L	0.0000
38	Q	L	0.0000
39	K	L	-1.3687
40	P	L	-1.1872
41	G	L	-1.2970
42	Q	L	-1.7572
43	A	L	-1.1291
44	P	L	0.0000
45	R	L	-1.2821
46	L	L	-0.4152
47	L	L	0.0000
48	I	L	0.0000
49	H	L	-0.3148
50	W	L	-0.1492
51	A	L	0.0000
52	S	L	-0.7328
53	T	L	-0.5536
54	R	L	-0.8522
55	A	L	0.1127
56	I	L	1.4937
57	G	L	0.3570
58	I	L	0.0000
59	P	L	-0.1926
60	A	L	-0.4596
61	R	L	-0.8339
62	F	L	0.0000
63	S	L	-0.6165
64	G	L	-0.5953
65	S	L	-0.7662
66	G	L	-1.2043
67	S	L	-0.9634
68	G	L	-1.2137
69	T	L	-1.8948
70	D	L	-2.6545
71	F	L	0.0000
72	T	L	-0.8525
73	L	L	0.0000
74	T	L	-0.6430
75	I	L	0.0000
76	S	L	-1.4664
77	S	L	-1.6956
78	L	L	0.0000
79	E	L	-2.2294
80	P	L	-1.6776
81	E	L	-2.2708
82	D	L	0.0000
83	F	L	-0.7503
84	A	L	0.0000
85	V	L	-0.2690
86	Y	L	0.0000
87	Y	L	0.0000
88	C	L	0.0000
89	Q	L	0.0000
90	Q	L	0.0000
91	G	L	0.0000
92	A	L	-0.0776
93	S	L	-0.3903
94	W	L	-0.2535
95	P	L	-0.4810
96	F	L	0.0000
97	T	L	-0.1484
98	F	L	0.0000
99	G	L	0.0000
100	Q	L	-1.3706
101	G	L	-1.0668
102	T	L	0.0000
103	K	L	-0.8663
104	V	L	0.0000
105	E	L	-0.7200
106	I	L	-0.5353
107	K	L	-0.7523
108	R	L	-0.7175
109	T	L	0.1317
110	V	L	0.7053
111	A	L	0.2779
112	A	L	-0.0459
113	P	L	0.0000
114	S	L	-0.1318
115	V	L	0.0000
116	F	L	0.1527
117	I	L	0.1613
118	F	L	0.0000
119	P	L	-0.5713
120	P	L	0.0000
121	S	L	-1.5565
122	D	L	-2.8482
123	E	L	-2.7798
124	Q	L	0.0000
125	L	L	-2.1847
126	K	L	-2.7483
127	S	L	-1.6997
128	G	L	-1.1962
129	T	L	-0.9229
130	A	L	0.0000
131	S	L	0.0000
132	V	L	0.0000
133	V	L	0.0000
134	C	L	0.0000
135	L	L	0.0000
136	L	L	0.0000
137	N	L	0.0000
138	N	L	-0.8762
139	F	L	0.0000
140	Y	L	0.0000
141	P	L	-1.6423
142	R	L	-3.0870
143	E	L	-3.2610
144	A	L	-2.3446
145	K	L	-2.4574
146	V	L	-1.2151
147	Q	L	-0.7769
148	W	L	0.0000
149	K	L	-0.7225
150	V	L	0.0000
151	D	L	-1.9269
152	N	L	-1.5398
153	A	L	-0.2961
154	L	L	0.6443
155	Q	L	-0.2436
156	S	L	-0.4459
157	G	L	-0.8870
158	N	L	-0.7661
159	S	L	-1.1865
160	Q	L	-1.4909
161	E	L	-2.0220
162	S	L	-1.0716
163	V	L	-0.8940
164	T	L	-1.2013
165	E	L	-2.2872
166	Q	L	-1.8837
167	D	L	-1.8428
168	S	L	-1.6637
169	K	L	-2.1479
170	D	L	-1.5314
171	S	L	-1.5807
172	T	L	0.0000
173	Y	L	0.0000
174	S	L	0.0000
175	L	L	0.0000
176	S	L	0.0000
177	S	L	0.0000
178	T	L	-0.6723
179	L	L	0.0000
180	T	L	-0.2931
181	L	L	-0.5166
182	S	L	-0.9302
183	K	L	-1.9909
184	A	L	-1.7437
185	D	L	-2.1980
186	Y	L	0.0000
187	E	L	-3.5591
188	K	L	-3.5806
189	H	L	-3.0056
190	K	L	-3.2750
191	V	L	-1.6593
192	Y	L	0.0000
193	A	L	0.0000
194	C	L	0.0000
195	E	L	-0.8530
196	V	L	0.0000
197	T	L	-1.2708
198	H	L	0.0000
199	Q	L	-1.7163
200	G	L	-0.3520
201	L	L	-0.2320
202	S	L	-0.4684
203	S	L	-0.4242
204	P	L	-0.6534
205	V	L	-0.0791
206	T	L	-0.5027
207	K	L	-0.6050
208	S	L	-0.5706
209	F	L	-0.8589
210	N	L	-2.0252
211	R	L	-2.4145
212	G	L	-1.3023
1	Q	C	-1.2117
2	L	C	0.0000
3	L	C	0.5482
4	F	C	-0.1664
5	N	C	-1.3131
6	K	C	-2.0041
7	T	C	-1.5340
8	K	C	-2.0333
9	S	C	-1.7802
10	V	C	-1.1287
11	E	C	-2.2950
12	F	C	0.0000
13	T	C	-0.3757
14	F	C	1.1710
15	G	C	-0.2987
16	N	C	-1.2758
17	D	C	-2.1203
18	T	C	-1.3533
19	V	C	0.0000
20	V	C	0.2263
21	I	C	0.0000
22	P	C	0.1935
23	C	C	0.0000
24	F	C	0.5756
25	V	C	0.0000
26	T	C	-0.2355
27	N	C	-0.4919
28	M	C	-1.0033
29	E	C	-2.4064
30	A	C	0.0000
31	Q	C	-2.3149
32	N	C	-2.0085
33	T	C	-0.9905
34	T	C	-1.0468
35	E	C	-1.0189
36	V	C	0.0000
37	Y	C	0.0000
38	V	C	0.0000
39	K	C	0.0000
40	W	C	0.0000
41	K	C	-1.8117
42	F	C	0.0000
43	K	C	-2.9503
44	G	C	-2.5520
45	R	C	-2.9500
46	D	C	-1.7720
47	I	C	0.0000
48	Y	C	0.0000
49	T	C	0.0000
50	F	C	0.0000
51	D	C	0.0000
52	G	C	0.0000
53	A	C	-0.6346
54	L	C	0.0000
55	N	C	-1.3336
56	K	C	-0.9490
57	S	C	-0.5891
58	T	C	-0.2454
59	V	C	-0.2901
60	P	C	0.0000
61	T	C	-0.9678
62	D	C	-1.9393
63	F	C	0.0000
64	S	C	-1.0548
65	S	C	-1.1638
66	A	C	0.0000
67	K	C	-1.7832
68	I	C	0.0000
69	E	C	-1.7209
70	V	C	-0.6943
71	S	C	-0.6878
72	Q	C	-1.0114
73	L	C	0.0000
74	L	C	-1.1098
75	K	C	-1.6827
76	G	C	0.0000
77	D	C	-0.3230
78	A	C	0.0000
79	S	C	0.0000
80	L	C	0.0000
81	K	C	-1.0757
82	M	C	0.0000
83	D	C	-1.5704
84	K	C	-0.7545
85	S	C	-0.7028
86	D	C	-0.7191
87	A	C	0.0000
88	V	C	1.4377
89	S	C	0.1877
90	H	C	-0.5387
91	T	C	-0.7482
92	G	C	-1.4747
93	N	C	-1.9629
94	Y	C	0.0000
95	T	C	-0.8463
96	C	C	0.0000
97	E	C	-1.1507
98	V	C	0.0000
99	T	C	0.0000
100	E	C	0.0000
101	L	C	-0.7461
102	T	C	-0.7561
103	R	C	-1.3348
104	E	C	-1.9040
105	G	C	-1.6567
106	E	C	-1.7160
107	T	C	0.0000
108	I	C	0.4136
109	I	C	0.0000
110	E	C	-1.7805
111	L	C	0.0000
112	K	C	-1.7553
113	Y	C	-0.6308
114	R	C	-1.8590