Project name: SH3_N136L

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	NA136L
Energy difference between WT (input) and mutated protein (by FoldX)	-0.26805 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.8199
• Total score value: -46.7338

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9233
88	V	A	0.4313
89	A	A	0.0000
90	L	A	0.2537
91	Y	A	-0.2899
92	D	A	-2.5603
93	Y	A	-1.9330
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7267
105	K	A	-2.4047
106	G	A	-1.4556
107	E	A	-1.3064
108	K	A	-0.6416
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0567
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1791
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1337
118	D	A	-1.9301
119	W	A	-0.6797
120	W	A	-0.7519
121	E	A	-1.1528
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4992
132	Y	A	-0.8671
133	I	A	0.0000
134	P	A	0.0000
135	S	A	0.4344
136	L	A	1.6647	mutated: NA136L
137	Y	A	1.2291
138	V	A	0.0000
139	A	A	0.9350
140	P	A	0.7757
141	V	A	1.7964