Project name: SH3_S135L

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA135L
Energy difference between WT (input) and mutated protein (by FoldX)	0.602825 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.9329
• Total score value: -53.1756

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5180
86	L	A	0.7941
87	F	A	0.9742
88	V	A	0.4338
89	A	A	0.0000
90	L	A	-0.1412
91	Y	A	-0.5700
92	D	A	-2.5578
93	Y	A	-1.9303
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7267
105	K	A	-2.4025
106	G	A	-1.4556
107	E	A	-1.3064
108	K	A	-0.6412
109	F	A	0.0000
110	Q	A	-0.5108
111	I	A	-0.0180
112	L	A	0.1387
113	N	A	-0.8880
114	S	A	-1.1089
115	S	A	-1.5813
116	E	A	-2.5365
117	G	A	-2.0291
118	D	A	-2.2999
119	W	A	-0.9755
120	W	A	-0.9653
121	E	A	-1.1145
122	A	A	0.0000
123	R	A	-1.7184
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6922
131	G	A	-1.5049
132	Y	A	-0.8249
133	I	A	0.0000
134	P	A	0.0000
135	L	A	-0.5302	mutated: SA135L
136	N	A	-0.9643
137	Y	A	0.0052
138	V	A	0.0000
139	A	A	0.4837
140	P	A	0.7757
141	V	A	1.7964