Project name: SH3_G131F

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA131F
Energy difference between WT (input) and mutated protein (by FoldX)	5.82632 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.9866
• Maximal score value: 1.7964
• Average score: -0.8994
• Total score value: -51.2655

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9238
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5615
93	Y	A	-1.9344
94	E	A	-2.6489
95	A	A	-2.5522
96	R	A	-2.9866
97	T	A	-2.6619
98	E	A	-2.9859
99	D	A	-2.8338
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.0955
103	F	A	0.0000
104	H	A	-2.7300
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5057
111	I	A	0.0167
112	L	A	0.2269
113	N	A	-0.8804
114	S	A	-1.1754
115	S	A	-1.5938
116	E	A	-2.5577
117	G	A	-2.1322
118	D	A	-2.4424
119	W	A	-1.0175
120	W	A	-0.9370
121	E	A	-0.9183
122	A	A	0.0000
123	R	A	-1.4978
124	S	A	0.0000
125	L	A	0.0877
126	T	A	-0.4467
127	T	A	-0.7650
128	G	A	-1.2022
129	E	A	-1.9110
130	T	A	-1.2563
131	F	A	-0.7651	mutated: GA131F
132	Y	A	-0.5210
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9197
136	N	A	-1.1500
137	Y	A	-0.1241
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964