Project name: SH3_L90K

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA90K
Energy difference between WT (input) and mutated protein (by FoldX)	0.427 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7894
• Average score: -1.055
• Total score value: -60.1369

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5269
86	L	A	0.8164
87	F	A	0.7475
88	V	A	0.0343
89	A	A	0.0000
90	K	A	-1.5777	mutated: LA90K
91	Y	A	-1.2623
92	D	A	-2.8491
93	Y	A	-2.1041
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.9506
105	K	A	-2.9011
106	G	A	-1.8322
107	E	A	-1.5269
108	K	A	-0.7963
109	F	A	0.0000
110	Q	A	-0.4951
111	I	A	-0.0510
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1026
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7001
124	S	A	0.0000
125	L	A	0.1083
126	T	A	-0.4482
127	T	A	-0.8060
128	G	A	-1.3409
129	E	A	-2.2315
130	T	A	-1.6868
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9204
136	N	A	-1.3504
137	Y	A	-0.5781
138	V	A	0.0000
139	A	A	0.0861
140	P	A	0.7689
141	V	A	1.7894