| Chain sequence(s) |
A: KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK |
| Distance of aggregation | 10 Å |
| Dynamic mode | No |
| Mutated residues | WA158D |
| Energy difference between WT (input) and mutated protein (by FoldX) | 5.28376 kcal/mol
CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 1 | K | A | -1.3020 | |
| 2 | I | A | 0.3034 | |
| 3 | E | A | -0.8970 | |
| 4 | E | A | -2.0316 | |
| 5 | G | A | -1.7104 | |
| 6 | K | A | -1.9345 | |
| 7 | L | A | 0.0000 | |
| 8 | V | A | -0.4643 | |
| 9 | I | A | 0.0000 | |
| 10 | W | A | 0.0000 | |
| 11 | I | A | 0.0000 | |
| 12 | N | A | -1.4370 | |
| 13 | G | A | -1.4412 | |
| 14 | D | A | -0.7962 | |
| 15 | K | A | -0.8520 | |
| 16 | G | A | 0.0000 | |
| 17 | Y | A | -0.8633 | |
| 18 | N | A | -1.6136 | |
| 19 | G | A | 0.0000 | |
| 20 | L | A | 0.0000 | |
| 21 | A | A | -1.5946 | |
| 22 | E | A | -2.7630 | |
| 23 | V | A | 0.0000 | |
| 24 | G | A | 0.0000 | |
| 25 | K | A | -3.9856 | |
| 26 | K | A | -3.5378 | |
| 27 | F | A | 0.0000 | |
| 28 | E | A | -3.7009 | |
| 29 | K | A | -4.0113 | |
| 30 | D | A | -3.5943 | |
| 31 | T | A | -2.3972 | |
| 32 | G | A | -2.3660 | |
| 33 | I | A | -2.1414 | |
| 34 | K | A | -2.9879 | |
| 35 | V | A | -1.4603 | |
| 36 | T | A | -0.6296 | |
| 37 | V | A | -0.4454 | |
| 38 | E | A | -1.2840 | |
| 39 | H | A | -1.7232 | |
| 40 | P | A | -2.2099 | |
| 41 | D | A | -3.2972 | |
| 42 | K | A | -3.4465 | |
| 43 | L | A | 0.0000 | |
| 44 | E | A | -2.1750 | |
| 45 | E | A | -2.8215 | |
| 46 | K | A | -2.7271 | |
| 47 | F | A | 0.0000 | |
| 48 | P | A | -1.3882 | |
| 49 | Q | A | -1.8003 | |
| 50 | V | A | -0.9649 | |
| 51 | A | A | 0.0000 | |
| 52 | A | A | -0.8594 | |
| 53 | T | A | -0.5803 | |
| 54 | G | A | -0.8758 | |
| 55 | D | A | -0.8970 | |
| 56 | G | A | -0.5927 | |
| 57 | P | A | 0.0000 | |
| 58 | D | A | 0.0000 | |
| 59 | I | A | 0.0000 | |
| 60 | I | A | 0.0000 | |
| 61 | F | A | 0.0000 | |
| 62 | W | A | 0.0115 | |
| 63 | A | A | -0.0502 | |
| 64 | H | A | 0.0000 | |
| 65 | D | A | 0.0000 | |
| 66 | R | A | -0.5792 | |
| 67 | F | A | 0.0000 | |
| 68 | G | A | 0.0000 | |
| 69 | G | A | -0.8761 | |
| 70 | Y | A | 0.0000 | |
| 71 | A | A | -1.5381 | |
| 72 | Q | A | -1.6412 | |
| 73 | S | A | -1.0650 | |
| 74 | G | A | -0.9890 | |
| 75 | L | A | 0.0000 | |
| 76 | L | A | 0.0000 | |
| 77 | A | A | -1.2012 | |
| 78 | E | A | -2.3196 | |
| 79 | I | A | 0.0000 | |
| 80 | T | A | -1.4552 | |
| 81 | P | A | 0.0000 | |
| 82 | D | A | -2.8808 | |
| 83 | K | A | -3.2599 | |
| 84 | A | A | -2.2207 | |
| 85 | F | A | -2.1258 | |
| 86 | Q | A | -2.4749 | |
| 87 | D | A | -3.1906 | |
| 88 | K | A | -2.4200 | |
| 89 | L | A | 0.0000 | |
| 90 | Y | A | -0.4408 | |
| 91 | P | A | -0.4759 | |
| 92 | F | A | 0.4997 | |
| 93 | T | A | 0.0000 | |
| 94 | W | A | 0.0000 | |
| 95 | D | A | -1.9686 | |
| 96 | A | A | 0.0000 | |
| 97 | V | A | 0.0000 | |
| 98 | R | A | -2.6421 | |
| 99 | Y | A | -2.2497 | |
| 100 | N | A | -2.2736 | |
| 101 | G | A | -2.3615 | |
| 102 | K | A | -3.1852 | |
| 103 | L | A | 0.0000 | |
| 104 | I | A | 0.0000 | |
| 105 | A | A | 0.0000 | |
| 106 | Y | A | 0.0000 | |
| 107 | P | A | 0.0000 | |
| 108 | I | A | 0.0000 | |
| 109 | A | A | 0.0000 | |
| 110 | V | A | 0.0000 | |
| 111 | E | A | 0.0000 | |
| 112 | A | A | 0.0000 | |
| 113 | L | A | 0.0000 | |
| 114 | S | A | 0.0000 | |
| 115 | L | A | 0.0000 | |
| 116 | I | A | 0.0000 | |
| 117 | Y | A | -0.4955 | |
| 118 | N | A | 0.0000 | |
| 119 | K | A | -2.0484 | |
| 120 | D | A | -2.3313 | |
| 121 | L | A | -1.2865 | |
| 122 | L | A | 0.0000 | |
| 123 | P | A | -1.6805 | |
| 124 | N | A | -1.9438 | |
| 125 | P | A | -1.1113 | |
| 126 | P | A | 0.0000 | |
| 127 | K | A | -2.1151 | |
| 128 | T | A | -1.8242 | |
| 129 | W | A | 0.0000 | |
| 130 | E | A | -1.7214 | |
| 131 | E | A | -1.5105 | |
| 132 | I | A | 0.0000 | |
| 133 | P | A | -1.5276 | |
| 134 | A | A | -1.2938 | |
| 135 | L | A | -1.3774 | |
| 136 | D | A | 0.0000 | |
| 137 | K | A | -3.2684 | |
| 138 | E | A | -3.3241 | |
| 139 | L | A | 0.0000 | |
| 140 | K | A | -3.2059 | |
| 141 | A | A | -2.4507 | |
| 142 | K | A | -2.9805 | |
| 143 | G | A | -2.4060 | |
| 144 | K | A | -2.6645 | |
| 145 | S | A | 0.0000 | |
| 146 | A | A | 0.0000 | |
| 147 | L | A | 0.0000 | |
| 148 | M | A | -0.1431 | |
| 149 | F | A | 0.0000 | |
| 150 | N | A | -0.5676 | |
| 151 | L | A | 0.0000 | |
| 152 | Q | A | -1.1522 | |
| 153 | E | A | -0.7362 | |
| 154 | P | A | 0.0000 | |
| 155 | Y | A | -0.2290 | |
| 156 | F | A | 0.0000 | |
| 157 | T | A | 0.0000 | |
| 158 | D | A | 0.0000 | mutated: WA158D |
| 159 | P | A | 0.0000 | |
| 160 | L | A | 0.0000 | |
| 161 | I | A | 0.0000 | |
| 162 | A | A | -0.5892 | |
| 163 | A | A | 0.0000 | |
| 164 | D | A | -1.7368 | |
| 165 | G | A | -0.8274 | |
| 166 | G | A | 0.0000 | |
| 167 | Y | A | -0.1591 | |
| 168 | A | A | 0.0000 | |
| 169 | F | A | 0.0000 | |
| 170 | K | A | -1.4032 | |
| 171 | Y | A | -0.8710 | |
| 172 | E | A | -2.8558 | |
| 173 | N | A | -2.7347 | |
| 174 | G | A | -2.1689 | |
| 175 | K | A | -2.5164 | |
| 176 | Y | A | -1.4903 | |
| 177 | D | A | -2.0163 | |
| 178 | I | A | -0.7814 | |
| 179 | K | A | -1.9452 | |
| 180 | D | A | -1.5671 | |
| 181 | V | A | 0.0000 | |
| 182 | G | A | 0.0000 | |
| 183 | V | A | 0.0000 | |
| 184 | D | A | -1.7828 | |
| 185 | N | A | -1.1805 | |
| 186 | A | A | -0.7347 | |
| 187 | G | A | 0.0000 | |
| 188 | A | A | 0.0000 | |
| 189 | K | A | -1.2111 | |
| 190 | A | A | -0.7609 | |
| 191 | G | A | 0.0000 | |
| 192 | L | A | 0.0000 | |
| 193 | T | A | -0.4346 | |
| 194 | F | A | -0.3891 | |
| 195 | L | A | 0.0000 | |
| 196 | V | A | -1.0553 | |
| 197 | D | A | -2.0856 | |
| 198 | L | A | 0.0000 | |
| 199 | I | A | -2.3753 | |
| 200 | K | A | -3.0951 | |
| 201 | N | A | -2.7617 | |
| 202 | K | A | -3.1308 | |
| 203 | H | A | -2.1221 | |
| 204 | M | A | 0.0000 | |
| 205 | N | A | -2.3241 | |
| 206 | A | A | -1.8887 | |
| 207 | D | A | -2.1479 | |
| 208 | T | A | 0.0000 | |
| 209 | D | A | -1.0270 | |
| 210 | Y | A | 0.0252 | |
| 211 | S | A | 0.1993 | |
| 212 | I | A | 0.6652 | |
| 213 | A | A | 0.0000 | |
| 214 | E | A | -0.4446 | |
| 215 | A | A | -0.6753 | |
| 216 | A | A | 0.0000 | |
| 217 | F | A | 0.0000 | |
| 218 | N | A | -2.1106 | |
| 219 | K | A | -2.6109 | |
| 220 | G | A | -2.1991 | |
| 221 | E | A | -2.7201 | |
| 222 | T | A | 0.0000 | |
| 223 | A | A | 0.0000 | |
| 224 | M | A | 0.0000 | |
| 225 | T | A | 0.0000 | |
| 226 | I | A | 0.0000 | |
| 227 | N | A | 0.0000 | |
| 228 | G | A | 0.0000 | |
| 229 | P | A | 0.0000 | |
| 230 | W | A | 0.0000 | |
| 231 | A | A | 0.0000 | |
| 232 | W | A | 0.0000 | |
| 233 | S | A | -1.7302 | |
| 234 | N | A | -1.8540 | |
| 235 | I | A | 0.0000 | |
| 236 | D | A | -2.4819 | |
| 237 | T | A | -1.7540 | |
| 238 | S | A | -2.0604 | |
| 239 | K | A | -2.6713 | |
| 240 | V | A | -2.0621 | |
| 241 | N | A | -1.8381 | |
| 242 | Y | A | -1.0660 | |
| 243 | G | A | -0.6527 | |
| 244 | V | A | 0.0509 | |
| 245 | T | A | -0.1282 | |
| 246 | V | A | -0.0620 | |
| 247 | L | A | 0.0000 | |
| 248 | P | A | 0.0000 | |
| 249 | T | A | -1.4654 | |
| 250 | F | A | 0.0000 | |
| 251 | K | A | -2.5017 | |
| 252 | G | A | -2.0858 | |
| 253 | Q | A | -1.9031 | |
| 254 | P | A | -1.2908 | |
| 255 | S | A | 0.0000 | |
| 256 | K | A | -1.1745 | |
| 257 | P | A | 0.0000 | |
| 258 | F | A | 0.0000 | |
| 259 | V | A | 0.0000 | |
| 260 | G | A | 0.0000 | |
| 261 | V | A | 0.0000 | |
| 262 | L | A | 0.0000 | |
| 263 | S | A | 0.0000 | |
| 264 | A | A | 0.0000 | |
| 265 | G | A | 0.0000 | |
| 266 | I | A | 0.0000 | |
| 267 | N | A | 0.0000 | |
| 268 | A | A | -0.7577 | |
| 269 | A | A | -0.6512 | |
| 270 | S | A | 0.0000 | |
| 271 | P | A | -1.0320 | |
| 272 | N | A | 0.0000 | |
| 273 | K | A | -1.6174 | |
| 274 | E | A | -2.4536 | |
| 275 | L | A | -1.4636 | |
| 276 | A | A | 0.0000 | |
| 277 | K | A | -2.2310 | |
| 278 | E | A | -2.4571 | |
| 279 | F | A | 0.0000 | |
| 280 | L | A | 0.0000 | |
| 281 | E | A | -1.4493 | |
| 282 | N | A | -1.8365 | |
| 283 | Y | A | -1.3484 | |
| 284 | L | A | 0.0000 | |
| 285 | L | A | 0.0000 | |
| 286 | T | A | -1.7063 | |
| 287 | D | A | -2.3290 | |
| 288 | E | A | -2.6202 | |
| 289 | G | A | 0.0000 | |
| 290 | L | A | 0.0000 | |
| 291 | E | A | -2.7951 | |
| 292 | A | A | -2.4367 | |
| 293 | V | A | 0.0000 | |
| 294 | N | A | -2.0121 | |
| 295 | K | A | -2.7009 | |
| 296 | D | A | -2.3018 | |
| 297 | K | A | -1.6973 | |
| 298 | P | A | -1.1005 | |
| 299 | L | A | 0.0000 | |
| 300 | G | A | 0.0000 | |
| 301 | A | A | 0.0000 | |
| 302 | V | A | 0.0000 | |
| 303 | A | A | 0.0000 | |
| 304 | L | A | 0.0000 | |
| 305 | K | A | -2.1954 | |
| 306 | S | A | -1.9672 | |
| 307 | Y | A | 0.0000 | |
| 308 | E | A | -2.2047 | |
| 309 | E | A | -3.3007 | |
| 310 | E | A | -3.3329 | |
| 311 | L | A | -2.2766 | |
| 312 | A | A | -2.1084 | |
| 313 | K | A | -2.6830 | |
| 314 | D | A | -1.7126 | |
| 315 | P | A | -1.0320 | |
| 316 | R | A | -0.8884 | |
| 317 | I | A | 0.0000 | |
| 318 | A | A | -0.8025 | |
| 319 | A | A | 0.0000 | |
| 320 | T | A | 0.0000 | |
| 321 | M | A | -0.7275 | |
| 322 | E | A | -1.7800 | |
| 323 | N | A | 0.0000 | |
| 324 | A | A | -1.3194 | |
| 325 | Q | A | -2.2294 | |
| 326 | K | A | -2.0660 | |
| 327 | G | A | -1.6799 | |
| 328 | E | A | -1.5056 | |
| 329 | I | A | -0.0475 | |
| 330 | M | A | 0.0000 | |
| 331 | P | A | 0.0000 | |
| 332 | N | A | -0.3195 | |
| 333 | I | A | 0.0000 | |
| 334 | P | A | -0.6566 | |
| 335 | Q | A | -0.3127 | |
| 336 | M | A | 0.0000 | |
| 337 | S | A | -0.0656 | |
| 338 | A | A | -0.1698 | |
| 339 | F | A | 0.0000 | |
| 340 | W | A | 0.0000 | |
| 341 | Y | A | 0.5431 | |
| 342 | A | A | 0.0000 | |
| 343 | V | A | 0.0000 | |
| 344 | R | A | -0.9158 | |
| 345 | T | A | -0.4990 | |
| 346 | A | A | 0.0000 | |
| 347 | V | A | 0.0000 | |
| 348 | I | A | -0.6750 | |
| 349 | N | A | -1.2710 | |
| 350 | A | A | 0.0000 | |
| 351 | A | A | 0.0000 | |
| 352 | S | A | -1.5401 | |
| 353 | G | A | -1.6976 | |
| 354 | R | A | -2.4555 | |
| 355 | Q | A | -2.1294 | |
| 356 | T | A | -1.9304 | |
| 357 | V | A | -2.0559 | |
| 358 | D | A | -3.4124 | |
| 359 | E | A | -3.7484 | |
| 360 | A | A | 0.0000 | |
| 361 | L | A | 0.0000 | |
| 362 | K | A | -4.0236 | |
| 363 | D | A | -3.7288 | |
| 364 | A | A | 0.0000 | |
| 365 | Q | A | -2.4973 | |
| 366 | T | A | -2.1396 | |
| 367 | R | A | -2.5056 | |
| 368 | I | A | 0.0000 | |
| 369 | T | A | -1.1693 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine