Project name: SH3_L112E

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112E
Energy difference between WT (input) and mutated protein (by FoldX)	1.4667 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1025
• Maximal score value: 1.795
• Average score: -1.1438
• Total score value: -65.1964

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4686
86	L	A	0.7090
87	F	A	0.8546
88	V	A	0.3873
89	A	A	0.0000
90	L	A	-0.1516
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6253
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1025
99	D	A	-3.0408
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2029
103	F	A	0.0000
104	H	A	-2.7285
105	K	A	-2.4065
106	G	A	-1.4592
107	E	A	-1.3295
108	K	A	-0.7050
109	F	A	0.0000
110	Q	A	-1.3672
111	I	A	-1.3840
112	E	A	-2.4539	mutated: LA112E
113	N	A	-2.1672
114	S	A	-1.7564
115	S	A	-1.9028
116	E	A	-2.5590
117	G	A	-2.1332
118	D	A	-2.4433
119	W	A	-1.1002
120	W	A	-1.5749
121	E	A	-1.9856
122	A	A	0.0000
123	R	A	-2.2165
124	S	A	0.0000
125	L	A	-0.0961
126	T	A	-0.5408
127	T	A	-0.8985
128	G	A	-1.5193
129	E	A	-2.2714
130	T	A	-2.0255
131	G	A	-1.7604
132	Y	A	-1.1191
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9176
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4150
140	P	A	0.7497
141	V	A	1.7950