Project name: SH3_W120F

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA120F
Energy difference between WT (input) and mutated protein (by FoldX)	2.14499 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8312
• Average score: -0.9434
• Total score value: -53.7742

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5437
86	L	A	0.8454
87	F	A	1.0264
88	V	A	0.4814
89	A	A	0.0000
90	L	A	-0.1385
91	Y	A	-0.5743
92	D	A	-2.5596
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4057
106	G	A	-1.4460
107	E	A	-1.2924
108	K	A	-0.6095
109	F	A	0.0000
110	Q	A	-0.4706
111	I	A	-0.0111
112	L	A	0.1616
113	N	A	-0.8626
114	S	A	-1.1544
115	S	A	-1.5812
116	E	A	-2.5370
117	G	A	-2.0995
118	D	A	-2.4170
119	W	A	-1.0624
120	F	A	-0.9779	mutated: WA120F
121	E	A	-1.1129
122	A	A	0.0000
123	R	A	-1.7044
124	S	A	0.0000
125	L	A	0.0697
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6913
131	G	A	-1.4851
132	Y	A	-0.8423
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8903
136	N	A	-1.1446
137	Y	A	-0.1130
138	V	A	0.0000
139	A	A	0.4499
140	P	A	0.8197
141	V	A	1.8312