Project name: SH3_G131L

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA131L
Energy difference between WT (input) and mutated protein (by FoldX)	5.54474 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0225
• Maximal score value: 1.7964
• Average score: -0.9231
• Total score value: -52.6181

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5137
86	L	A	0.7912
87	F	A	0.9195
88	V	A	0.4291
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5611
93	Y	A	-1.9337
94	E	A	-2.6480
95	A	A	-2.5755
96	R	A	-2.9862
97	T	A	-2.6616
98	E	A	-3.0225
99	D	A	-2.9008
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1425
103	F	A	0.0000
104	H	A	-2.7295
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6401
109	F	A	0.0000
110	Q	A	-0.5107
111	I	A	-0.0082
112	L	A	0.2013
113	N	A	-0.8941
114	S	A	-1.1824
115	S	A	-1.5977
116	E	A	-2.5621
117	G	A	-2.1357
118	D	A	-2.4464
119	W	A	-1.0507
120	W	A	-0.9814
121	E	A	-1.0022
122	A	A	0.0000
123	R	A	-1.5599
124	S	A	0.0000
125	L	A	0.0729
126	T	A	-0.4626
127	T	A	-0.8030
128	G	A	-1.2776
129	E	A	-2.0958
130	T	A	-1.4234
131	L	A	-1.0103	mutated: GA131L
132	Y	A	-0.6311
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9227
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7739
141	V	A	1.7964