| Chain sequence(s) |
A: KVRWPDFNQEAYVGGTMVRSGQDPYARNKFNQVESDKLRMDRAIPDTRHDQCQRKQWRVDLPATSVVITFHNEARSALLRTVVSVLKKSPPHLIKEIILVDDYSNDPEDGALLGKIEKVRVLRNDRREGLMRSRVRGADAAQAKVLTFLDSHCECNEHWLEPLLERVAEDRTRVVSPIIDVINMDNFQYVGASADLKGGFDWNLVFKWDYMTPEQRRSRQGNPVAPIKTPMIAGGLFVMDKFYFEELGKYDMMMDVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAAEVWMDEYKNFYYAAVPSARNVPYGNIQSRLELRKKLSCKPFKWYLENVYPELRVPDHQDIAFGALQQGTNCLDTLGHFADGVVGVYECHNAGGNQEWALTKEKSVKHMDLCLTVVDRAPGSLIKLQGCRENDSRQKWEQIEGNSKLRHVGSNLCLDSRTAKSGGLSVEVCGPALSQQWKFTLN P: STTPAPTTK |
| Distance of aggregation | 5 Å |
| Dynamic mode | No |
| Mutated residues | LA421P |
| Energy difference between WT (input) and mutated protein (by FoldX) | 5.40369 kcal/mol
CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 75 | K | A | -1.6535 | |
| 76 | V | A | -0.4056 | |
| 77 | R | A | -1.7759 | |
| 78 | W | A | -0.2702 | |
| 79 | P | A | -0.5639 | |
| 80 | D | A | -1.8364 | |
| 81 | F | A | 0.0000 | |
| 82 | N | A | -1.2975 | |
| 83 | Q | A | -0.7042 | |
| 84 | E | A | -1.8335 | |
| 85 | A | A | -0.2768 | |
| 86 | Y | A | 0.0000 | |
| 87 | V | A | 0.0000 | |
| 88 | G | A | -0.4403 | |
| 89 | G | A | -0.5339 | |
| 90 | T | A | 0.0440 | |
| 91 | M | A | 0.8094 | |
| 92 | V | A | 0.0835 | |
| 93 | R | A | -1.8308 | |
| 94 | S | A | -0.6411 | |
| 95 | G | A | -0.6141 | |
| 96 | Q | A | -1.0008 | |
| 97 | D | A | -1.9082 | |
| 98 | P | A | -0.3225 | |
| 99 | Y | A | 0.3061 | |
| 100 | A | A | -0.1673 | |
| 101 | R | A | -1.5586 | |
| 102 | N | A | 0.0000 | |
| 103 | K | A | -0.3900 | |
| 104 | F | A | 0.0000 | |
| 105 | N | A | -0.1320 | |
| 106 | Q | A | 0.0000 | |
| 107 | V | A | 0.7361 | |
| 108 | E | A | -0.1281 | |
| 109 | S | A | 0.0000 | |
| 110 | D | A | -0.4961 | |
| 111 | K | A | -1.6816 | |
| 112 | L | A | -0.3734 | |
| 113 | R | A | -1.7717 | |
| 114 | M | A | -0.2747 | |
| 115 | D | A | -0.2590 | |
| 116 | R | A | -0.2589 | |
| 117 | A | A | 0.0685 | |
| 118 | I | A | 0.2578 | |
| 119 | P | A | -0.4629 | |
| 120 | D | A | -1.8249 | |
| 121 | T | A | -0.3850 | |
| 122 | R | A | -0.3638 | |
| 123 | H | A | -0.9421 | |
| 124 | D | A | -2.0057 | |
| 125 | Q | A | -0.9410 | |
| 126 | C | A | 0.0000 | |
| 127 | Q | A | -1.5376 | |
| 128 | R | A | -2.2135 | |
| 129 | K | A | -1.3707 | |
| 130 | Q | A | -1.2801 | |
| 131 | W | A | 0.0099 | |
| 132 | R | A | -0.3445 | |
| 133 | V | A | 1.3049 | |
| 134 | D | A | -1.4621 | |
| 135 | L | A | 0.0000 | |
| 136 | P | A | -0.0270 | |
| 137 | A | A | 0.0080 | |
| 138 | T | A | 0.0000 | |
| 139 | S | A | 0.0000 | |
| 140 | V | A | 0.0000 | |
| 141 | V | A | 0.0000 | |
| 142 | I | A | 0.0000 | |
| 143 | T | A | -0.0077 | |
| 144 | F | A | 0.0000 | |
| 145 | H | A | -0.2304 | |
| 146 | N | A | -0.4374 | |
| 147 | E | A | 0.0000 | |
| 148 | A | A | 0.0000 | |
| 149 | R | A | 0.0000 | |
| 150 | S | A | 0.0000 | |
| 151 | A | A | 0.0000 | |
| 152 | L | A | 0.0000 | |
| 153 | L | A | 0.0000 | |
| 154 | R | A | 0.0000 | |
| 155 | T | A | 0.0000 | |
| 156 | V | A | 0.0000 | |
| 157 | V | A | 0.0000 | |
| 158 | S | A | 0.0000 | |
| 159 | V | A | 0.0000 | |
| 160 | L | A | -0.0557 | |
| 161 | K | A | -1.2934 | |
| 162 | K | A | -0.5422 | |
| 163 | S | A | 0.0000 | |
| 164 | P | A | -0.0890 | |
| 165 | P | A | -0.4446 | |
| 166 | H | A | -1.0369 | |
| 167 | L | A | 0.0000 | |
| 168 | I | A | 0.0000 | |
| 169 | K | A | -1.3377 | |
| 170 | E | A | 0.0000 | |
| 171 | I | A | 0.0000 | |
| 172 | I | A | 0.0000 | |
| 173 | L | A | 0.0000 | |
| 174 | V | A | 0.0000 | |
| 175 | D | A | 0.0000 | |
| 176 | D | A | 0.0000 | |
| 177 | Y | A | 0.9473 | |
| 178 | S | A | 0.0000 | |
| 179 | N | A | -1.4550 | |
| 180 | D | A | -1.2548 | |
| 181 | P | A | -0.5965 | |
| 182 | E | A | -1.4653 | |
| 183 | D | A | -0.4395 | |
| 184 | G | A | 0.0000 | |
| 185 | A | A | -0.0132 | |
| 186 | L | A | 0.4976 | |
| 187 | L | A | 0.0000 | |
| 188 | G | A | -0.3761 | |
| 189 | K | A | -1.6526 | |
| 190 | I | A | 0.0000 | |
| 191 | E | A | -0.3529 | |
| 192 | K | A | -0.7529 | |
| 193 | V | A | 0.0000 | |
| 194 | R | A | -0.4360 | |
| 195 | V | A | 0.3732 | |
| 196 | L | A | 0.2087 | |
| 197 | R | A | -0.5163 | |
| 198 | N | A | 0.0000 | |
| 199 | D | A | -2.1275 | |
| 200 | R | A | -2.2218 | |
| 201 | R | A | -0.6877 | |
| 202 | E | A | -0.4816 | |
| 203 | G | A | 0.0000 | |
| 204 | L | A | 0.1828 | |
| 205 | M | A | 0.0000 | |
| 206 | R | A | -0.3764 | |
| 207 | S | A | 0.0000 | |
| 208 | R | A | -0.1434 | |
| 209 | V | A | 0.0033 | |
| 210 | R | A | -0.7517 | |
| 211 | G | A | 0.0000 | |
| 212 | A | A | 0.0000 | |
| 213 | D | A | -1.7866 | |
| 214 | A | A | -0.3137 | |
| 215 | A | A | 0.0000 | |
| 216 | Q | A | -1.1983 | |
| 217 | A | A | 0.0000 | |
| 218 | K | A | -0.8818 | |
| 219 | V | A | 0.0000 | |
| 220 | L | A | 0.0000 | |
| 221 | T | A | 0.0000 | |
| 222 | F | A | 0.0000 | |
| 223 | L | A | 0.0000 | |
| 224 | D | A | -0.5371 | |
| 225 | S | A | 0.0000 | |
| 226 | H | A | 0.0000 | |
| 227 | C | A | 0.0000 | |
| 228 | E | A | 0.0000 | |
| 229 | C | A | 0.0000 | |
| 230 | N | A | 0.0000 | |
| 231 | E | A | -1.4998 | |
| 232 | H | A | -0.7499 | |
| 233 | W | A | 0.0000 | |
| 234 | L | A | 0.0000 | |
| 235 | E | A | 0.0000 | |
| 236 | P | A | -0.0263 | |
| 237 | L | A | 0.0000 | |
| 238 | L | A | 0.0000 | |
| 239 | E | A | -0.2976 | |
| 240 | R | A | -0.3181 | |
| 241 | V | A | 0.0000 | |
| 242 | A | A | -0.3159 | |
| 243 | E | A | -1.8799 | |
| 244 | D | A | -1.2760 | |
| 245 | R | A | -1.5754 | |
| 246 | T | A | -0.3400 | |
| 247 | R | A | -0.2462 | |
| 248 | V | A | 0.0000 | |
| 249 | V | A | 0.0000 | |
| 250 | S | A | 0.0000 | |
| 251 | P | A | 0.0000 | |
| 252 | I | A | 0.0000 | |
| 253 | I | A | 0.0000 | |
| 254 | D | A | 0.0000 | |
| 255 | V | A | 0.1829 | |
| 256 | I | A | 0.0000 | |
| 257 | N | A | -0.6834 | |
| 258 | M | A | -0.1929 | |
| 259 | D | A | -1.2393 | |
| 260 | N | A | -1.4499 | |
| 261 | F | A | 0.0000 | |
| 262 | Q | A | -1.1983 | |
| 263 | Y | A | 0.0000 | |
| 264 | V | A | 0.5293 | |
| 265 | G | A | 0.0000 | |
| 266 | A | A | 0.0000 | |
| 267 | S | A | -0.2922 | |
| 268 | A | A | -0.0792 | |
| 269 | D | A | -0.5411 | |
| 270 | L | A | 0.0000 | |
| 271 | K | A | -0.2486 | |
| 272 | G | A | 0.0000 | |
| 273 | G | A | 0.0000 | |
| 274 | F | A | 0.0000 | |
| 275 | D | A | -1.1432 | |
| 276 | W | A | 0.0000 | |
| 277 | N | A | -0.4830 | |
| 278 | L | A | 0.0000 | |
| 279 | V | A | 0.6319 | |
| 280 | F | A | 0.0000 | |
| 281 | K | A | -0.9761 | |
| 282 | W | A | -0.1021 | |
| 283 | D | A | -0.2752 | |
| 284 | Y | A | 1.0638 | |
| 285 | M | A | 0.0000 | |
| 286 | T | A | -0.0560 | |
| 287 | P | A | -0.5668 | |
| 288 | E | A | -2.0946 | |
| 289 | Q | A | -0.6974 | |
| 290 | R | A | -1.1288 | |
| 291 | R | A | -2.2095 | |
| 292 | S | A | -0.5957 | |
| 293 | R | A | -0.5012 | |
| 294 | Q | A | -1.3289 | |
| 295 | G | A | -0.7895 | |
| 296 | N | A | -0.6527 | |
| 297 | P | A | -0.1893 | |
| 298 | V | A | 0.0000 | |
| 299 | A | A | 0.0006 | |
| 300 | P | A | -0.0645 | |
| 301 | I | A | 0.0000 | |
| 302 | K | A | -1.2698 | |
| 303 | T | A | 0.0000 | |
| 304 | P | A | 0.0000 | |
| 305 | M | A | 0.0000 | |
| 306 | I | A | 0.0000 | |
| 307 | A | A | 0.0000 | |
| 308 | G | A | 0.0000 | |
| 309 | G | A | -0.0823 | |
| 310 | L | A | 0.0000 | |
| 311 | F | A | 0.0000 | |
| 312 | V | A | 0.0000 | |
| 313 | M | A | 0.0000 | |
| 314 | D | A | 0.0000 | |
| 315 | K | A | 0.1473 | |
| 316 | F | A | 1.9023 | |
| 317 | Y | A | 0.0000 | |
| 318 | F | A | 0.0000 | |
| 319 | E | A | -1.1449 | |
| 320 | E | A | -1.9595 | |
| 321 | L | A | 0.0000 | |
| 322 | G | A | -0.1483 | |
| 323 | K | A | -0.6084 | |
| 324 | Y | A | 0.0000 | |
| 325 | D | A | 0.0000 | |
| 326 | M | A | 0.4756 | |
| 327 | M | A | 0.8928 | |
| 328 | M | A | 0.0000 | |
| 329 | D | A | -0.3173 | |
| 330 | V | A | 0.0000 | |
| 331 | W | A | 0.1691 | |
| 332 | G | A | 0.0000 | |
| 333 | G | A | 0.0000 | |
| 334 | E | A | 0.0000 | |
| 335 | N | A | -0.1450 | |
| 336 | L | A | 0.0000 | |
| 337 | E | A | 0.0000 | |
| 338 | I | A | 0.0000 | |
| 339 | S | A | 0.0000 | |
| 340 | F | A | 0.0000 | |
| 341 | R | A | 0.0000 | |
| 342 | V | A | 0.0000 | |
| 343 | W | A | 0.0000 | |
| 344 | Q | A | 0.0000 | |
| 345 | C | A | 0.0240 | |
| 346 | G | A | -0.4486 | |
| 347 | G | A | 0.0000 | |
| 348 | S | A | -0.0335 | |
| 349 | L | A | 0.0000 | |
| 350 | E | A | 0.0000 | |
| 351 | I | A | 0.0000 | |
| 352 | I | A | 0.0000 | |
| 353 | P | A | 0.0000 | |
| 354 | C | A | 0.0000 | |
| 355 | S | A | 0.0000 | |
| 356 | R | A | 0.0000 | |
| 357 | V | A | 0.0000 | |
| 358 | G | A | 0.0000 | |
| 359 | H | A | 0.0000 | |
| 360 | V | A | 0.0000 | |
| 361 | F | A | 0.0000 | |
| 362 | R | A | -0.5560 | |
| 363 | K | A | -1.9223 | |
| 364 | Q | A | -1.2975 | |
| 365 | H | A | -0.3034 | |
| 366 | P | A | -0.0500 | |
| 367 | Y | A | 0.1752 | |
| 368 | T | A | 0.0019 | |
| 369 | F | A | 0.1112 | |
| 370 | P | A | -0.3057 | |
| 371 | G | A | -0.5546 | |
| 372 | G | A | -0.3017 | |
| 373 | S | A | -0.2036 | |
| 374 | G | A | -0.4878 | |
| 375 | T | A | -0.1261 | |
| 376 | V | A | 0.0000 | |
| 377 | F | A | 0.3319 | |
| 378 | A | A | 0.0000 | |
| 379 | R | A | -0.7847 | |
| 380 | N | A | 0.0000 | |
| 381 | T | A | 0.0000 | |
| 382 | R | A | -0.2201 | |
| 383 | R | A | 0.0000 | |
| 384 | A | A | 0.0000 | |
| 385 | A | A | 0.0000 | |
| 386 | E | A | -0.3063 | |
| 387 | V | A | 0.0000 | |
| 388 | W | A | 0.0000 | |
| 389 | M | A | 0.0000 | |
| 390 | D | A | -0.8192 | |
| 391 | E | A | -1.8603 | |
| 392 | Y | A | -0.1118 | |
| 393 | K | A | -0.4164 | |
| 394 | N | A | -1.2450 | |
| 395 | F | A | 0.1492 | |
| 396 | Y | A | 0.0000 | |
| 397 | Y | A | 0.1735 | |
| 398 | A | A | 0.0916 | |
| 399 | A | A | 0.1284 | |
| 400 | V | A | 0.3889 | |
| 401 | P | A | -0.2071 | |
| 402 | S | A | -0.2573 | |
| 403 | A | A | 0.0000 | |
| 404 | R | A | -1.6193 | |
| 405 | N | A | -1.5283 | |
| 406 | V | A | 0.0000 | |
| 407 | P | A | -0.2085 | |
| 408 | Y | A | 0.1189 | |
| 409 | G | A | -0.6564 | |
| 410 | N | A | -1.3598 | |
| 411 | I | A | 0.0000 | |
| 412 | Q | A | -1.1894 | |
| 413 | S | A | -0.4343 | |
| 414 | R | A | 0.0000 | |
| 415 | L | A | 0.0262 | |
| 416 | E | A | -1.6879 | |
| 417 | L | A | -0.1936 | |
| 418 | R | A | -0.5339 | |
| 419 | K | A | -2.0596 | |
| 420 | K | A | -2.0238 | |
| 421 | P | A | -0.4134 | mutated: LA421P |
| 422 | S | A | -0.2071 | |
| 423 | C | A | -0.1035 | |
| 424 | K | A | -0.9277 | |
| 425 | P | A | -0.2553 | |
| 426 | F | A | 0.0000 | |
| 427 | K | A | -1.6545 | |
| 428 | W | A | -0.0652 | |
| 429 | Y | A | 0.0000 | |
| 430 | L | A | 0.0000 | |
| 431 | E | A | -2.0821 | |
| 432 | N | A | -1.5745 | |
| 433 | V | A | -0.0464 | |
| 434 | Y | A | 0.0000 | |
| 435 | P | A | -0.6872 | |
| 436 | E | A | -1.3238 | |
| 437 | L | A | -0.3986 | |
| 438 | R | A | -1.6596 | |
| 439 | V | A | 0.4527 | |
| 440 | P | A | -0.3027 | |
| 441 | D | A | -1.9946 | |
| 442 | H | A | -1.5418 | |
| 443 | Q | A | -1.7127 | |
| 444 | D | A | -1.8480 | |
| 445 | I | A | 0.5623 | |
| 446 | A | A | 0.3188 | |
| 447 | F | A | 0.7436 | |
| 448 | G | A | 0.0000 | |
| 449 | A | A | 0.0069 | |
| 450 | L | A | 0.0000 | |
| 451 | Q | A | -0.2472 | |
| 452 | Q | A | 0.0000 | |
| 453 | G | A | -0.4794 | |
| 454 | T | A | -0.2429 | |
| 455 | N | A | -0.7099 | |
| 456 | C | A | 0.0000 | |
| 457 | L | A | 0.0000 | |
| 458 | D | A | 0.0000 | |
| 459 | T | A | 0.0000 | |
| 460 | L | A | 0.6296 | |
| 461 | G | A | -0.4145 | |
| 462 | H | A | -0.1597 | |
| 463 | F | A | 1.8699 | |
| 464 | A | A | 0.0628 | |
| 465 | D | A | -1.7937 | |
| 466 | G | A | -0.3528 | |
| 467 | V | A | 0.2457 | |
| 468 | V | A | 0.0000 | |
| 469 | G | A | 0.0000 | |
| 470 | V | A | 0.6497 | |
| 471 | Y | A | 1.0868 | |
| 472 | E | A | -1.4437 | |
| 473 | C | A | -0.2794 | |
| 474 | H | A | -0.8900 | |
| 475 | N | A | -1.3878 | |
| 476 | A | A | -0.1979 | |
| 477 | G | A | -0.2634 | |
| 478 | G | A | -0.6125 | |
| 479 | N | A | -0.7602 | |
| 480 | Q | A | 0.0000 | |
| 481 | E | A | -0.6076 | |
| 482 | W | A | 0.0000 | |
| 483 | A | A | 0.0000 | |
| 484 | L | A | 0.2308 | |
| 485 | T | A | -0.2818 | |
| 486 | K | A | -2.0361 | |
| 487 | E | A | -2.2681 | |
| 488 | K | A | -1.0768 | |
| 489 | S | A | -0.1577 | |
| 490 | V | A | 0.0000 | |
| 491 | K | A | -0.3294 | |
| 492 | H | A | 0.0000 | |
| 493 | M | A | 0.7247 | |
| 494 | D | A | -1.5959 | |
| 495 | L | A | 0.0000 | |
| 496 | C | A | 0.0000 | |
| 497 | L | A | 0.0000 | |
| 498 | T | A | 0.0000 | |
| 499 | V | A | 0.0000 | |
| 500 | V | A | 0.4949 | |
| 501 | D | A | -1.4925 | |
| 502 | R | A | -1.8628 | |
| 503 | A | A | -0.3006 | |
| 504 | P | A | -0.3037 | |
| 505 | G | A | -0.2214 | |
| 506 | S | A | 0.0191 | |
| 507 | L | A | 0.4242 | |
| 508 | I | A | 0.0000 | |
| 509 | K | A | -0.3188 | |
| 510 | L | A | 0.0000 | |
| 511 | Q | A | -0.1654 | |
| 512 | G | A | -0.1033 | |
| 513 | C | A | -0.0165 | |
| 514 | R | A | -1.3205 | |
| 515 | E | A | -2.2428 | |
| 516 | N | A | -1.6397 | |
| 517 | D | A | -0.4483 | |
| 518 | S | A | -0.3569 | |
| 519 | R | A | -0.6471 | |
| 520 | Q | A | 0.0000 | |
| 521 | K | A | -0.2397 | |
| 522 | W | A | 0.0000 | |
| 523 | E | A | -0.3857 | |
| 524 | Q | A | -0.8215 | |
| 525 | I | A | -0.3647 | |
| 526 | E | A | -1.8651 | |
| 527 | G | A | -1.0402 | |
| 528 | N | A | -1.4190 | |
| 529 | S | A | -0.3297 | |
| 530 | K | A | -0.2635 | |
| 531 | L | A | 0.0000 | |
| 532 | R | A | -0.5477 | |
| 533 | H | A | 0.0000 | |
| 534 | V | A | 0.4131 | |
| 535 | G | A | -0.3825 | |
| 536 | S | A | -0.3712 | |
| 537 | N | A | -1.2821 | |
| 538 | L | A | 0.0000 | |
| 539 | C | A | 0.0000 | |
| 540 | L | A | 0.0000 | |
| 541 | D | A | 0.0000 | |
| 542 | S | A | -0.2771 | |
| 543 | R | A | -1.3826 | |
| 544 | T | A | -0.2790 | |
| 545 | A | A | -0.2933 | |
| 546 | K | A | -1.7354 | |
| 547 | S | A | -0.5837 | |
| 548 | G | A | -0.3485 | |
| 549 | G | A | 0.0000 | |
| 550 | L | A | 0.0000 | |
| 551 | S | A | 0.0000 | |
| 552 | V | A | 0.0000 | |
| 553 | E | A | -0.0508 | |
| 554 | V | A | 1.1362 | |
| 555 | C | A | 0.3782 | |
| 556 | G | A | -0.1414 | |
| 557 | P | A | -0.2740 | |
| 558 | A | A | 0.2615 | |
| 559 | L | A | 1.5445 | |
| 560 | S | A | 0.2428 | |
| 561 | Q | A | 0.0000 | |
| 562 | Q | A | -0.7451 | |
| 563 | W | A | 0.0000 | |
| 564 | K | A | -1.6200 | |
| 565 | F | A | 0.1161 | |
| 566 | T | A | 0.3498 | |
| 567 | L | A | 1.3095 | |
| 568 | N | A | -0.9869 | |
| 5 | S | P | -0.2144 | |
| 6 | T | P | -0.0497 | |
| 7 | T | P | -0.0300 | |
| 8 | P | P | -0.0806 | |
| 9 | A | P | 0.0250 | |
| 10 | P | P | 0.0000 | |
| 11 | T | P | -0.0542 | |
| 12 | T | P | -0.3544 | |
| 13 | K | P | -1.7093 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine