Project name: SH3_Y132R

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA132R
Energy difference between WT (input) and mutated protein (by FoldX)	1.46603 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.2068
• Maximal score value: 1.7964
• Average score: -1.0042
• Total score value: -57.24

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9231
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6963
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1689
99	D	A	-3.2068
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2758
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.4806
111	I	A	-0.0489
112	L	A	0.2359
113	N	A	-0.7939
114	S	A	-1.1918
115	S	A	-1.5720
116	E	A	-2.5779
117	G	A	-2.1960
118	D	A	-2.5833
119	W	A	-1.4108
120	W	A	-1.2972
121	E	A	-1.4571
122	A	A	0.0000
123	R	A	-1.7982
124	S	A	0.0000
125	L	A	0.0591
126	T	A	-0.4730
127	T	A	-0.8208
128	G	A	-1.3575
129	E	A	-2.2407
130	T	A	-1.8246
131	G	A	-1.8189
132	R	A	-1.4705	mutated: YA132R
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0629
136	N	A	-1.1717
137	Y	A	-0.1397
138	V	A	0.0000
139	A	A	0.4149
140	P	A	0.7757
141	V	A	1.7964