Project name: SH3_E107N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA107N
Energy difference between WT (input) and mutated protein (by FoldX)	0.299045 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1049
• Maximal score value: 1.799
• Average score: -0.9259
• Total score value: -52.7778

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5161
86	L	A	0.7936
87	F	A	0.9612
88	V	A	0.5074
89	A	A	0.0000
90	L	A	-0.1158
91	Y	A	-0.5465
92	D	A	-2.5327
93	Y	A	-1.9372
94	E	A	-2.6525
95	A	A	-2.6330
96	R	A	-2.9880
97	T	A	-2.6629
98	E	A	-3.1049
99	D	A	-3.0453
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2235
103	F	A	0.0000
104	H	A	-2.6611
105	K	A	-2.3336
106	G	A	-1.3450
107	N	A	0.0000	mutated: EA107N
108	K	A	-0.5205
109	F	A	0.0000
110	Q	A	-0.5093
111	I	A	-0.0552
112	L	A	0.1430
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1529
122	A	A	0.0000
123	R	A	-1.7170
124	S	A	0.0000
125	L	A	0.0945
126	T	A	-0.4226
127	T	A	-0.7980
128	G	A	-1.3624
129	E	A	-2.2449
130	T	A	-1.6962
131	G	A	-1.5016
132	Y	A	-0.8696
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4178
140	P	A	0.7774
141	V	A	1.7990