Project name: SH3_S114T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA114T
Energy difference between WT (input) and mutated protein (by FoldX)	1.12628 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7956
• Average score: -0.9586
• Total score value: -54.6429

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5094
86	L	A	0.7825
87	F	A	0.9132
88	V	A	0.4301
89	A	A	0.0000
90	L	A	-0.1514
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4584
107	E	A	-1.3082
108	K	A	-0.6480
109	F	A	0.0000
110	Q	A	-0.5322
111	I	A	-0.0840
112	L	A	0.1395
113	N	A	-0.8625
114	T	A	-1.1098	mutated: SA114T
115	S	A	-1.5597
116	E	A	-2.5420
117	G	A	-2.1260
118	D	A	-2.4342
119	W	A	-1.0946
120	W	A	-1.0483
121	E	A	-1.1522
122	A	A	0.0000
123	R	A	-1.7276
124	S	A	0.0000
125	L	A	0.0589
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6977
131	G	A	-1.5061
132	Y	A	-0.8675
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9157
136	N	A	-1.1513
137	Y	A	-0.1241
138	V	A	0.0000
139	A	A	0.4153
140	P	A	0.7746
141	V	A	1.7956