Project name: SH3_L125V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA125V
Energy difference between WT (input) and mutated protein (by FoldX)	1.71976 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8458
• Average score: -0.9294
• Total score value: -52.9747

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5361
86	L	A	0.9202
87	F	A	1.0096
88	V	A	0.5196
89	A	A	0.0000
90	L	A	-0.1433
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7191
105	K	A	-2.3956
106	G	A	-1.4390
107	E	A	-1.1697
108	K	A	-0.4253
109	F	A	0.0000
110	Q	A	-0.5469
111	I	A	-0.1233
112	L	A	0.0906
113	N	A	-0.9099
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0776
121	E	A	-1.1838
122	A	A	0.0000
123	R	A	-1.6559
124	S	A	0.0000
125	V	A	0.5423	mutated: LA125V
126	T	A	-0.2159
127	T	A	-0.6749
128	G	A	-1.2367
129	E	A	-2.1691
130	T	A	-1.6720
131	G	A	-1.5047
132	Y	A	-0.8707
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4424
140	P	A	0.7995
141	V	A	1.8458