Project name: SH3_S135D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA135D
Energy difference between WT (input) and mutated protein (by FoldX)	1.6458 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.9828
• Total score value: -56.0198

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5180
86	L	A	0.7941
87	F	A	0.8998
88	V	A	0.4505
89	A	A	0.0000
90	L	A	-0.1118
91	Y	A	-0.5436
92	D	A	-2.5411
93	Y	A	-1.9103
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.3893
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6422
109	F	A	0.0000
110	Q	A	-0.5092
111	I	A	-0.0909
112	L	A	0.1414
113	N	A	-0.9090
114	S	A	-1.2612
115	S	A	-1.6176
116	E	A	-2.5919
117	G	A	-2.2278
118	D	A	-2.6088
119	W	A	-1.3082
120	W	A	-1.2188
121	E	A	-1.2262
122	A	A	0.0000
123	R	A	-1.7168
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6922
131	G	A	-1.5143
132	Y	A	-0.9475
133	I	A	0.0000
134	P	A	0.0000
135	D	A	-1.2375	mutated: SA135D
136	N	A	-1.2603
137	Y	A	-0.1132
138	V	A	0.0000
139	A	A	0.3891
140	P	A	0.7757
141	V	A	1.7964