Project name: SH3_K105D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	KA105D
Energy difference between WT (input) and mutated protein (by FoldX)	1.80715 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8043
• Average score: -0.92
• Total score value: -52.438

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5179
86	L	A	0.7946
87	F	A	0.9555
88	V	A	0.5617
89	A	A	0.0000
90	L	A	0.1549
91	Y	A	-0.3352
92	D	A	-2.3886
93	Y	A	-1.8540
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.5265
105	D	A	-1.9923	mutated: KA105D
106	G	A	-1.1921
107	E	A	-1.1496
108	K	A	-0.5610
109	F	A	0.0000
110	Q	A	-0.5083
111	I	A	-0.0569
112	L	A	0.1429
113	N	A	-0.8877
114	S	A	-1.1790
115	S	A	-1.5957
116	E	A	-2.5591
117	G	A	-2.1323
118	D	A	-2.4437
119	W	A	-1.1023
120	W	A	-1.0554
121	E	A	-1.1528
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0598
126	T	A	-0.4667
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4992
132	Y	A	-0.8674
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9205
136	N	A	-1.1251
137	Y	A	-0.0239
138	V	A	0.0000
139	A	A	0.4638
140	P	A	0.7773
141	V	A	1.8043